茎柔鱼眼睛晶体微量元素地理差异及其与水温关系

许巍; 刘必林; 陈新军; 陈勇; 操亮亮; 桓梦瑶

doi:10.12284/hyxb2021102

茎柔鱼眼睛晶体微量元素地理差异及其与水温关系

doi: 10.12284/hyxb2021102

许巍^1,,
刘必林^{1, 2, 3, 4, ,},
陈新军^{1, 2, 3, 4},
陈勇^{1, 5},
操亮亮¹,
桓梦瑶¹

1.
上海海洋大学海洋科学学院，上海 201306
2.
国家远洋渔业工程技术研究中心，上海 201306
3.
大洋渔业资源可持续开发教育部重点实验室，上海 201306
4.
农业农村部大洋渔业开发重点实验室，上海 201306
5.
农业农村部大洋渔业资源环境科学观测实验站，上海 201306

基金项目: 国家重点研发计划（2019YFD0901404）；国家自然科学基金（41876141）；上海市高校特聘教授“东方学者”岗位计划项目（0810000243）；上海市科技创新行动计划（19DZ1207502）

详细信息

作者简介:
许巍（1980-），男，江苏省镇江市人，博士，从事渔业资源学研究。E-mial：wxu@shou.edu.cn

通讯作者:
刘必林，男，教授，主要从事渔业资源学研究。E-mail：bl-liu@shou.edu.cn

中图分类号: S917; S931
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出版历程
- 收稿日期: 2021-01-31
- 修回日期: 2021-02-27
- 网络出版日期: 2021-04-29
- 刊出日期: 2021-06-30

Geographical differences and their relationship with sea surface temperature of trace elements in the eye lenses of Jumbo flying squid (Dosidicus gigas)

Xu Wei^1
,,
Liu Bilin^{1, 2, 3, 4
, ,},
Chen Xinjun^{1, 2, 3, 4},
Chen Yong^{1, 5},
Cao Liangliang¹,
Huan Mengyao¹

1.
College of Marine Sciences, Shanghai Ocean University, Shanghai 201306, China
2.
National Engineering Research Center for Oceanic Fisheries, Shanghai 201306, China
3.
The Key Laboratory of Sustainable Exploitation of Oceanic Fisheries Resources, Ministry of Education, Shanghai 201306, China
4.
Key Laboratory of Oceanic Fisheries Exploration, Ministry of Agriculture and Rural Affairs, Shanghai 201306, China
5.
Scientific Observing and Experimental Station of Oceanic Fishery Resources, Ministry of Agriculture and Rural Affairs, Shanghai 201306, China

摘要

摘要: 本研究以2015年和2017年我国远洋鱿钓渔船在东南太平洋的厄瓜多尔、秘鲁和智利公海生产时采集的茎柔鱼眼睛晶体为研究材料，采用LA-ICPMS测定了眼睛晶体外缘的微量元素，比较微量元素地理区域间差异，分析微量元素浓度与茎柔鱼栖息水温的关系。结果显示，厄瓜多尔、秘鲁和智利公海的茎柔鱼眼睛晶体外缘Mg²⁵、Ni⁶⁰ 、Cu⁶³、Sr⁸⁸和Ba¹³⁷等元素在3个海区之间存在显著差异(p<0.05)，Na²³、Al²⁷、Si²⁹、P³¹、Ca⁴³、Mn⁵⁵、Zn⁶⁶和Pb等元素在两两海区之间均不存在显著差异(p>0.05)。除了Sr⁸⁸、Ba¹³⁷、Fe⁵⁷和Ni⁶⁰与海表面温度呈显著的线性负相关，其他元素与海表面温度均无显著的相关关系。分析认为，Sr⁸⁸、Ba¹³⁷、Fe⁵⁷和Ni⁶⁰均可以看作茎柔鱼生活水温的指示元素，Ba¹³⁷元素还可以看作茎柔鱼生活水深以及上升流的指示元素。研究认为，眼睛晶体微量元素可用于头足类栖息环境的重建。
- 茎柔鱼 /
- 眼睛晶体 /
- 微量元素 /
- 海表面温度 /
- 东南太平洋
Abstract: Samples of Jumbo flying squid (Dosidicus gigas) were collected from Chinese squid jigging vessels in the high seas of Ecuador, Peru and Chile in the southeast Pacific Ocean in 2015 and 2017. The trace elements in the eye lenses of D. gigas were measured to study the geographical differences and their relationship with sea surface temperature. The results showed that elements such as Mg²⁵, Ni⁶⁰, Cu⁶³, Sr⁸⁸ and Ba¹³⁷in the peripheral part of eye lenses of D. gigas in the high seas of Ecuador, Peru and Chile were significant difference, while there were no significant differences between each two sea areas in the elements of Na²³, Al²⁷, Si²⁹, P³¹, Ca⁴³, Mn⁵⁵, Zn⁶⁶ and Pb. Except for Sr⁸⁸, Ba¹³⁷, Fe⁵⁷ and Ni⁶⁰, which showed significantly linear negative correlations with SST (p<0.05), there were no significant correlations with SST in the other elements (p>0.05). Sr⁸⁸, Ba¹³⁷, Fe⁵⁷ and Ni⁶⁰ could be regarded as indicator elements of the ambient temperature of D. gigas, and Ba¹³⁷ could also be regarded as indicator element of the water depth and upwelling. Consequently, trace elements in eye lenses could be used to reconstruct the habitat environment of cephalopod.
- Jumbo flying squid /
- eye lenses /
- trace elements /
- sea surface temperature /
- Southeastern Pacific Ocean

HTML全文

图 1 厄瓜多尔、秘鲁和智利公海茎柔鱼采样站点分布

Fig. 1 Sampling locations of Dosidicus gigas in the high seas of Ecuador, Peru and Chile

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图 2 茎柔鱼眼睛晶体示意图

Fig. 2 Diagram of eye lens of Dosidicus gigas

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图 3 眼睛晶体切片制作流程

a. 眼睛晶体后部；b. 塑料磨具；c. 经过冷埋树脂包埋的眼睛晶体；d−f. 切割后的树脂块黏于载玻片上并研磨至赤道面

Fig. 3 Flow charts of eye lens preparation

a. Posterior eye lens; b. plastic moulding; c. posterior eye lens embedded in the resin; d−f. the resin block is adhered to the slide and ground to the equatorial surface

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图 4 茎柔鱼眼睛晶体外缘取样点

Fig. 4 Spot in peripheral part of eye lens formed at newly ontogenetic phase of Dosidicus gigas

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图 5 Sr⁸⁸、Ba¹³⁷、Fe⁵⁷和Ni⁶⁰浓度与海表面温度呈显著的负相关关系

Fig. 5 The negative relationships between SST with concentration of Sr⁸⁸、Ba¹³⁷、Fe⁵⁷and Ni⁶⁰

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表 1 厄瓜多尔、秘鲁和智利公海茎柔鱼样本信息

Tab. 1 Sampling information of Dosidicus gigas in the high seas of Ecuador, Peru and Chile

海区	采样日期	采样地点	样本量/尾	胴长/mm	体质量/g
厄瓜多尔	2017年7−8月	3°21′～8°26′S，84°07′～91°52′W	10	210～357	264～1 295
秘鲁	2015年6−9月	9°16′～15°22′S，79°45′～85°03′W	13	226～352	280～1 301
智利	2015年11−12月	37°06′～40°00′S，79°00′～83°00′W	5	301～470	538～3 012

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表 2 厄瓜多尔、秘鲁和智利公海茎柔鱼眼睛晶体外缘微量元素浓度

Tab. 2 Concentration of trace elements in peripheral eye lenses of Dosidicus gigas in the high seas of Ecuador, Peru and Chile

元素	浓度/10⁻⁶
元素	三海区总体	厄瓜多尔	秘鲁	智利
Na²³	28 397±23 103	19 991±14 855^a	31 662±28 466^a	36 720±19 430^a
Mg²⁵	10 629±9 992	3 843±4 420^a	13 875±11 586^b	15 762±6 707^b
Al²⁷	96 260±125 462	131 503±133 837^a	75 553±140 866^a	79 615±39 657^a
Si²⁹	33 878±19 111	31 156±19 894^a	39 253±20 382^a	25 348±11 186^a
P³¹	247 985±111 951	221 034±140 584^a	268 912±109 203^a	247 474±33 373^a
K³⁹	15 484±12 565	8 167±5 436^a	20 487±1 585^b	17 110±5 166^ab
Ca⁴³	68 303±95 317	90 218±155 137^a	43 776±20 838^a	88 240±47 102^a
Mn⁵⁵	510±971	188±286^a	534±1 241^a	1 091±933^a
Fe⁵⁷	1 169±1 272	1 099±757^ab	790±834^a	2 296±234^b
Ni⁶⁰	264±357	218±216^a	131±210^a	701±567^b
Cu⁶³	1 177±1 122	1 836±1 494^a	623±604^b	1 302±523^ab
Zn⁶⁶	2 051±1 601	1 195±1 203^a	2 404±1 895^a	2 845±492^a
Sr⁸⁸	137±124	75±71^a	138±109^a	257±170^b
Ba¹³⁷	77±144	56±80^a	29±49^a	245±273^b
Pb	34±21	27±13^a	37±27^a	45±14^a
注：以a、b表示海区间元素浓度差异水平 (p<0.05)。

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表 3 厄瓜多尔、秘鲁和智利公海茎柔鱼眼睛晶外缘微量元素方差分析结果

Tab. 3 ANOVA results of trace elements in peripheral eye lenses of Dosidicus gigas in the high seas of Ecuador, Peru and Chile

元素	方差平方和	均方差	自由度1	自由度2	F	p
Na²³	1.19×10⁹	5.96×10⁸	2	26	1.127	0.34
Mg²⁵	7.29×10⁸	3.65×10⁸	2	26	4.635	0.019
Al²⁷	1.94×10¹⁰	9.69×10⁹	2	26	0.597	0.558
Si²⁹	8.14×10⁸	4.07×10⁸	2	26	1.124	0.341
P³¹	1.30×10¹⁰	6.48×10⁹	2	26	0.498	0.614
K³⁹	8.74×10⁸	4.37×10⁹	2	26	3.224	0.057
Ca⁴³	1.46×10¹⁰	7.31×10⁹	2	26	0.792	0.464
Mn⁵⁵	2 734 390.992	1 367 195.496	2	26	1.506	0.241
Fe⁵⁷	8 268 121.63	4 134 060.815	2	26	2.917	0.073
Ni⁶⁰	1 207 061.18	603 530.59	2	26	6.755	0.005
Cu⁶³	8 410 317.756	4 205 158.878	2	26	4.112	0.029
Zn⁶⁶	12 099 595.63	6 049 797.816	2	26	2.649	0.09
Sr⁸⁸	110 045.401	55 022.701	2	26	4.513	0.021
Ba¹³⁷	174 712.54	87 356.27	2	26	5.679	0.009
Pb	1 397.56	698.78	2	26	1.589	0.224

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