Gonadal development of the sea cucumber Acaudina molpadioides in north coast of Fujian
-
摘要: 海地瓜（Acaudina molpadioides）是一种低值海参，本研究运用组织学和形态学方法对福建北部海域海地瓜的性腺发育特征及其周年变化进行了研究，并进一步探究了性别比、生殖周期、精卵排放类型、繁殖季节等繁殖生物学相关内容。结果表明，雌、雄海地瓜的性腺发育均可划分为休止期 (I)、发育早期 (II)、增长期 (III)、成熟期 (IV)、部分排放期 (V) 和排空期 (VI)，雌、雄性腺发育程度基本同步。海地瓜性腺发育有明显的周年变化规律：大多数海地瓜在5月性成熟后开始陆续排放配子，配子排放过程中伴随着生殖管的自体破坏然后重生，即性腺开始新一轮发育，新的生殖细胞在11月开始生成并逐步发育直至翌年5月成熟。海地瓜性别比接近1∶1，生殖周期为1年，繁殖期为5−8月（夏季），属于多次排精卵类型。Abstract: Acaudina molpadioides is a low-value sea cucumber. We studied the characteristics and annual variation of gonadal development of A. molpadioides in north coast of Fujian based on histological and morphological observation. We continued to explore its gender ratio, reproductive cycle, spawning pattern and reproductive season. Results showed that the testis and ovary development of A. molpadioides could be divided into six stages: resting stage (I), early developmental stage (II), growing stage (III), mature stage (IV), partly spawned stage (V) and spent stage (VI). The gonadal development of A. molpadioides in males and females were basically synchronous, and it followed an obvious annual development rule. The majority of gonads matured in May. Subsequently, gametes were being released meanwhile with tubules destruction. Then new tubules grew up, which meant the gonad started a new cycle. The gametogenesis initiated in November, and gametes gradually developed till matured in next May. A. molpadioides had a gender ratio close to 1∶1 and an annual reproductive cycle. Its reproductive season was from May to August (summer), when it spawned several times.
-
Key words:
- Acaudina molpadioides /
- gonadal development /
- annual variation /
- reproductive season
-
图 2 海地瓜的性腺
a. 分枝状的生殖管；b. 成熟精巢；c. 成熟卵巢
Fig. 2 Gonad of A. molpadioides
a. Branched tubule；b. mature testis；c. mature ovary
图 3 海地瓜精巢不同发育阶段的组织结构特征
a. 休止期（I）；b, c. 发育早期（II）；d, e. 增长期（III）；f, g. 成熟期（IV）；h. 部分排放期（V）；i. 排空期（VI）；TW. 生殖管壁；SG. 精原细胞；SC. 精母细胞；SZ. 成熟精子；RS. 残留精子
Fig. 3 Histological features of A. molpadioides testis at different stages
a. Resting stage (I)；b,c. early developmental stage (II)；d,e. growing stage (III)；f,g. mature stage (IV)；h. partly spawned stage (V)；i. spent stage (VI)；TW. tubule wall；SG. spermatogonium；SC. spermatocyte；SZ. mature spermatozoa；RS. residual spermatozoa
图 4 海地瓜卵巢不同发育阶段的组织结构特征
a. 休止期（I）；b. 发育早期（er）；c. 增长期（III）；d. 成熟期（IV）；e. 部分排放期（V）；f. 排空期（VI）；O. 卵原细胞；PVO. 卵黄发生前卵母细胞；VO. 卵黄发生期卵母细胞；MO. 成熟卵细胞；RO. 残留卵母细胞；PH. 吞噬细胞
Fig. 4 Histological features of A. molpadioides ovary at different stages
a. Resting stage (I)；b. early developmental stage (Ⅱ)；c. growing stage (III)；d. mature stage (IV)；e. partly spawned stage (V)；f. spent stage (VI)；O. oogonia；PVO. previtellogenic oocyte；VO. vitellogenic oocyte；MO. mature ootid；RO. residual oocyte；PH. phagocyte
图 5 海地瓜精巢（a）和卵巢（b）发育的周年变化
Fig. 5 Annual variation of testis (a) and ovary (b) development in A. molpadioides
-
[1] 廖玉麟. 中国动物志: 棘皮动物门(海参纲)[M]. 北京: 科学出版社, 1997.Liao Yulin. Fauna Sinica: Echinodermata (Holothuroidea)[M]. Beijing: Science Press, 1997. [2] 苏永昌, 刘淑集, 刘秋凤, 等. 海地瓜营养成分的分析及评价[J]. 渔业研究, 2016, 38(4): 288−294.Su Yongchang, Liu Shuji, Liu Qiufeng, et al. Analysis and evaluation of nutritional components in Acaudina molpadioides[J]. Journal of Fisheries Research, 2016, 38(4): 288−294. [3] 董晓弟, 潘如佳, 王长海. 海地瓜, 黑乳参和乌皱辐肛参营养成分对比[J]. 现代食品科技, 2013, 29(12): 2986−2990.Dong Xiaodi, Pan Rujia, Wang Changhai. Comparison of nutritional values of Acaudina molpadioides, Holothurian nobilis, and Actinopyga miliaris[J]. Modern Food Science and Technology, 2013, 29(12): 2986−2990. [4] 王宁丽, 于培良, 赵立春, 等. 不同产地海地瓜活性物质检测及降糖活性评价[J]. 中国海洋药物, 2019, 38(2): 11−16.Wang Ningli, Yu Peiliang, Zhao Lichun, et al. Determination of active substances and evaluation of hypoglycemic activity in Acaudina molpadioides from different producing areas[J]. Chinese Journal of Marine Drugs, 2019, 38(2): 11−16. [5] 陈发河, 李真, 吴光斌. 海地瓜多糖和多肽提取纯化工艺及抗氧化活性[J]. 集美大学学报(自然科学版), 2018, 23(2): 105−118.Chen Fahe, Li Zhen, Wu Guangbin. Study on the extraction and antioxidant activities of polysaccharides and polypeptides from Acaudina molpadioides (Semper)[J]. Journal of Jimei University (Natural Science), 2018, 23(2): 105−118. [6] Wang Y C, Su W, Zhang C Y, et al. Protective effect of sea cucumber (Acaudina molpadioides) fucoidan against ethanol-induced gastric damage[J]. Food Chemistry, 2012, 133(4): 1414−1419. doi: 10.1016/j.foodchem.2012.02.028 [7] Liu X F, Xu J, Xue Y, et al. Sea cucumber cerebrosides and long-chain bases from Acaudina molpadioides protect against high fat diet-induced metabolic disorders in mice[J]. Food & Function, 2015, 6(11): 3428−3436. [8] 吴彦农, 王娅琦, 候秦脉, 等. 海洋异物堵塞核电厂取水系统事件的经验反馈[J]. 核安全, 2017, 16(1): 26−32.Wu Yannong, Wang Yaqi, Hou Qinmai, et al. Experience feedbacks on events of nuclear power plants cold source systems blocked by oceanic foreign matter[J]. Nuclear Safety, 2017, 16(1): 26−32. [9] 於凡, 许波涛, 李勇, 等. 海生物暴发对核电厂冷源系统的影响分析及对策探讨[J]. 给水排水, 2018, 54(2): 61−64. doi: 10.3969/j.issn.1002-8471.2018.02.013Yu Fan, Xu Botao, Li Yong, et al. Impact analysis and countermeasure discussion for marine organisms outbreak at nuclear power plants cold source systems[J]. Water & Wastewater Engineering, 2018, 54(2): 61−64. doi: 10.3969/j.issn.1002-8471.2018.02.013 [10] Barath Kumar S, Mohanty A K, Das N P I, et al. Impingement of marine organisms in a tropical atomic power plant cooling water system[J]. Marine Pollution Bulletin, 2017, 124(1): 555−562. doi: 10.1016/j.marpolbul.2017.07.067 [11] Masilamoni J G, Jesudoss K S, Nandakumar K, et al. Jellyfish ingress: a threat to the smooth operation of coastal power plants[J]. Current Science, 2000, 79(5): 567−569. [12] 张凤瀛, 廖玉麟, 吴宝铃, 等. 中国动物图谱: 棘皮动物[M]. 北京: 科学出版社, 1964: 46.Zhang Fengying, Liao Yulin, Wu Baoling, et al. Animal Atlas of China: Echinodermata[M]. Beijing: Science Press, 1964: 46. [13] Ramofafia C, Byrne M, Battaglene C S. Reproduction of the commercial sea cucumber Holothuria scabra (Echinodermata: Holothuroidea) in the Solomon Islands[J]. Marine Biology, 2003, 142(2): 281−288. doi: 10.1007/s00227-002-0947-x [14] 杨学明, 吴明灿, 张立, 等. 海南糙海参性腺发育的周年变化[J]. 南方农业学报, 2015, 46(6): 1117−1122. doi: 10.3969/j.issn.2095-1191.2015.6.1117Yang Xueming, Wu Mingcan, Zhang Li, et al. Annual change of gonadal development in Holothuria scabra from Hainan Island[J]. Journal of Southern Agriculture, 2015, 46(6): 1117−1122. doi: 10.3969/j.issn.2095-1191.2015.6.1117 [15] Asha P S, Muthiah P. Reproductive biology of the commercial sea cucumber Holothuria spinifera (Echinodermata: Holothuroidea) from Tuticorin, Tamil Nadu, India[J]. Aquaculture International, 2008, 16(3): 231−242. doi: 10.1007/s10499-007-9140-z [16] Hamel J F, Himmelman J H, Dufresne L. Gametogenesis and spawning of the sea cucumber Psolus fabricii (Duben and Koren)[J]. The Biological Bulletin, 1993, 184(2): 125−143. doi: 10.2307/1542223 [17] Cameron J L, Fankboner P V. Reproductive biology of the commercial sea cucumber Parastichopus californicus (Stimpson) (Echinodermata: Holothuroidea). I. Reproductive periodicity and spawning behavior[J]. Canadian Journal of Zoology, 1986, 64: 168−175. doi: 10.1139/z86-027 [18] 张立, 杨学明, 江林源, 等. 海南岛糙海参性腺发育的组织学特征[J]. 南方水产科学, 2016, 12(3): 51−57. doi: 10.3969/j.issn.2095-0780.2016.03.007Zhang Li, Yang Xueming, Jiang Linyuan, et al. Histological characteristics of gonadal development of Holothuria scabra from Hainan Island[J]. South China Fisheries Science, 2016, 12(3): 51−57. doi: 10.3969/j.issn.2095-0780.2016.03.007 [19] Ramofafia C, Battaglene C S, Bell J D, et al. Reproductive biology of the commercial sea cucumber Holothuria fuscogilva in the Solomon Islands[J]. Marine Biology, 2000, 136(6): 1045−1056. doi: 10.1007/s002270000310 [20] Giese A C, Pearse J S, Pearse V B. Reproduction of Marine Invertebrates: Echinoderms and Lophophorates[M]. California: Boxwood Press, 1991. [21] Sewell M A, Bergquist P R. Variability in the reproductive cycle of Stichopus mollis (Echinodermata: Holothuroidea)[J]. Invertebrate Reproduction and Development, 1990, 17(1): 1−7. doi: 10.1080/07924259.1990.9672081 [22] 庞震国. 刺参的性腺发育及受精细胞学研究[D]. 青岛: 中国海洋大学, 2006.Pang Zhenguo. Reproductive biology and fertilization research on Apostichopus japonicus[D]. Qingdao: Ocean University of China, 2006. [23] 隋锡林, 刘永襄, 刘永峰, 等. 刺参生殖周期的研究[J]. 水产学报, 1985, 9(4): 303−310.Sui Xilin, Liu Yongxiang, Liu Yongfeng, et al. A study on the reproductive cycle of sea cucumber[J]. Journal of Fisheries of China, 1985, 9(4): 303−310. [24] Morgan A D. Aspects of the reproductive cycle of the sea cucumber Holothuria scabra (Echinodermata: Holothuroidea)[J]. Bulletin of Marine Science, 2000, 66(1): 47−57. [25] Thorson G. Reproduction and larval ecology of marine bottom invertebrates[J]. Biological Reviews of the Cambridge Philosophical Society, 1950, 25(1): 1−45. doi: 10.1111/j.1469-185X.1950.tb00585.x [26] Conand C. Reproductive biology of the holothurians from the major communities of the New Caledonian Lagoon[J]. Marine Biology, 1993, 116(3): 439−450. doi: 10.1007/BF00350061 -
下载:
图(6)
计量
- 文章访问数: 1149
- HTML全文浏览量: 288
- PDF下载量: 77
- 被引次数: 0
