降解聚乙烯塑料芽孢杆菌LC-2的分离鉴定及降解特性研究

江婷婷; 丁慧平; 冯丽娟; 张大海; 李苓; 刘彦东; 李先国

doi:10.12284/hyxb2021036

降解聚乙烯塑料芽孢杆菌LC-2的分离鉴定及降解特性研究

doi: 10.12284/hyxb2021036

江婷婷^{1, 2,},
丁慧平^{1, 2},
冯丽娟^{1, 2},
张大海^{1, 2},
李苓^{1, 2},
刘彦东^{1, 2},
李先国^{1, 2, ,}

1.
中国海洋大学化学化工学院，山东青岛 266100
2.
海洋化学理论与工程技术教育部重点实验室，山东青岛 266100

基金项目: 中央高校基本科研业务费专项（201861015）。

详细信息

作者简介:
江婷婷(1995-)，女，安徽省马鞍山市人，主要研究方向为环境分析化学。E-mail：1871600481@qq.com

通讯作者:
李先国(1965-)，男，教授，主要研究方向为海洋有机地球化学及现代有机污染物与生物地球化学。E-mail：lixg@ouc.edu.cn

中图分类号: X172
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出版历程
- 收稿日期: 2020-08-30
- 修回日期: 2020-10-26
- 网络出版日期: 2021-01-21
- 刊出日期: 2021-03-02

Isolation, identification and polyethylene-degrading characteristics of Bacillus LC-2

Jiang Tingting^{1, 2
,},
Ding Huiping^{1, 2},
Feng Lijuan^{1, 2},
Zhang Dahai^{1, 2},
Li Ling^{1, 2},
Liu Yandong^{1, 2},
Li Xianguo^{1, 2
, ,}

1.
College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China
2.
Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Qingdao 266100, China

摘要

摘要: 塑料在环境中不断地积累，逐渐破碎成为尺寸小于5 mm的微塑料，对环境和人类健康构成严重威胁。本研究从青岛李村河口采集的塑料薄膜上分离出一株能够降解聚乙烯(PE)的细菌，命名为LC-2，通过分子生物学结合形态学和生理生化特征分析将其鉴定为芽孢杆菌(Bacillus aquimaris)。在以PE为唯一碳源的液体培养基中，接种该细菌培养28 d后，通过扫描电镜、接触角测定、热重分析以及傅里叶变换红外光谱等手段分析表明，PE的失重率在9%左右，表面形貌发生变化，疏水性变小且表面发生氧化，产生−C=O官能团，这些证据足以证明LC-2可以降解PE。
- 聚乙烯 /
- 塑料 /
- 降解 /
- 芽孢杆菌
Abstract: Plastics accumulate in the environment and gradually break into microplastics (MPs, size below 5 mm). It poses a serious threat to the environment and human health. In this study, a strain LC-2 capable of degrading polyethylene (PE) was isolated from plastic films collected from the Licun Estuary of Qingdao. It was identified as Bacillus aquimaris by using a combination of molecular biological technique with morphological, physiological and biochemical characteristics. After the strain was isolated, it was cultured in a liquid medium for 28 days with PE as the sole carbon source. The polyethylene was subsequently separated and examined with a scanning electron microscopy, a contact angle test, a thermogravimetric analysis and a Fourier transform infrared spectrum analysis. The results showed that the weight loss of PE was about 9% after the degradation, with apparent morphologic changes on the surface of PE. It was also showed that the hydrophobicity of PE became smaller, with surface oxidation to produce −C=O functional groups. These evidences are sufficient to prove that the strain LC-2 can degrade PE.
- polyethylene /
- plastic /
- degradation /
- Bacillus aquimaris

HTML全文

图 1 基于16S rRNA基因序列构建的菌株LC-2系统发育树

只展示自展值大于50%的节点数据，邻接法重复取样1 000次

Fig. 1 Neighbor-Joining tree of strain LC-2 constructed based on 16S rRNA gene sequence

Numbers at nodes indicate bootstrap values (>50%) based on a neighbor-joining analysis of 1 000 resampled datasets

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图 2 PE塑料的失重率曲线

Fig. 2 Weight loss curve of PE plastic

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图 3 培养基中游离的细菌数

Fig. 3 The number of free state bacteria in the medium

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图 4 PE塑料的扫描电镜照片

a. 未接种LC-2的PE培养28 d后；b−d. 接种LC-2的PE 培养28 d后，显示出明显的孔洞（b）、裂痕（c）和凹坑（d）

Fig. 4 Scanning electron microscopy photographs of PE plastic

a. After 28 days in culture medium without LC-2 inoculated; b−d. after 28 days in culture medium with LC-2 inoculated showing the appearance of holes (b), cracks (c) and pits (d)

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图 5 PE塑料的水接触角图片

a. 对照组28 d后；b. 实验组28 d后

Fig. 5 The photographs of water contact angles of PE plastic

a. Control group after 28 days; b. experimental group after 28 days

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图 6 PE塑料降解前后的红外光谱图

Fig. 6 FTIR spectra of PE plastic before and after degradation

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图 7 PE塑料的热重曲线

Fig. 7 Thermogravimetric curve of PE plastic

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