低氧胁迫对军曹鱼幼鱼生长、血清生化和非特异性免疫指标的影响

王维政; 曾泽乾; 黄建盛; 郭志雄; 李洪娟; 陈刚

doi:10.12284/hyxb2021012

低氧胁迫对军曹鱼幼鱼生长、血清生化和非特异性免疫指标的影响

doi: 10.12284/hyxb2021012

王维政^1,,
曾泽乾¹,
黄建盛^{1, 2, 3, ,},
郭志雄¹,
李洪娟¹,
陈刚^{1, 2, 3}

1.
广东海洋大学水产学院，广东湛江 524088
2.
南方海洋科学与工程广东省实验室（湛江），广东湛江 524025
3.
广东省水产经济动物病原生物学及流行病学重点实验室，广东湛江 524088

基金项目: 现代农业产业技术体系专项资金（CARS-47）；南方海洋科学与工程广东省实验室（湛江）资助项目（ZJW-2019-06）；广东海洋大学大学生创新创业训练计划项目（CXXL2018131）。

详细信息

作者简介:
王维政（1995—），男，广东省广州市人，主要研究方向为海水鱼类养殖生理生态学。E-mail：1029588896@qq.com

通讯作者:
黄建盛，副教授，主要研究方向为海水鱼养殖生理生态学。E-mail：huangjs@gdou.edu.cn

中图分类号: P714⁺.5
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- 被引次数: 0
出版历程
- 收稿日期: 2020-05-02
- 修回日期: 2020-08-14
- 网络出版日期: 2021-01-27
- 刊出日期: 2021-03-02

Hypoxia stress on growth, serum biochemical and non-specific immune indexes of juvenile cobia (Rachycentron canadum)

Wang Weizheng^1
,,
Zeng Zeqian¹,
Huang Jiansheng^{1, 2, 3
, ,},
Guo Zhixiong¹,
Li Hongjuan¹,
Chen Gang^{1, 2, 3}

1.
Fishery College, Guangdong Ocean University, Zhanjiang 524088, China
2.
Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang 524025, China
3.
Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524088, China

摘要

摘要: 为了研究低氧胁迫对军曹鱼幼鱼生长、血清生化和非特异性免疫指标的影响，将体重为(50.44±2.78) g的军曹鱼幼鱼在(3.15±0.21) mg/L的低氧环境下养殖4周，分别在第1天、第7天、第14天、第28天取样并进行相关指标的测定。结果表明，在低氧胁迫过程中：(1)军曹鱼幼鱼生长受到低氧的抑制，增重率和特定生长率显著低于对照水平(p<0.05)。(2)血清肝功能指标表现为不同程度的上升趋势，其中谷草转氨酶(AST)在第7天、第14天、第28天极显著高于对照组(p<0.01)；谷丙转氨酶活性在第14天、第28天极显著高于对照组(p<0.01)；总蛋白和白蛋白含量均在第14天和第28天与对照组有显著性差异(p<0.05)。(3)血清糖脂水平表现为波动升高的趋势，其中血糖含量在第1天、第14天和第28天与对照水平有显著性差异(p<0.05)；甘油三酯含量在第14天和第28天极显著高于对照水平(p<0.01)；总胆固醇含量在第1天显著下降(p<0.05)后呈不断上升的趋势。(4)血清离子含量变化情况各异，其中钠离子和氯离子含量在第14天和第28天极显著升高(p<0.01)；钾离子含量不断升高并在第7天、第14天和第28天与对照组具有显著性差异(p<0.05)；钙离子含量则呈不断下降的变化趋势，在第14天和第28天显著低于对照水平(p<0.05)。(5)血清超氧化物歧化酶活性持续升高，并在第7天、第14天、第28天极显著高于对照组(p<0.01)；丙二醛含量持续上升，并在第14天、第28天极显著高于对照组(p<0.01)；过氧化氢酶活性表现为“下降−上升−下降”变化趋势；溶菌酶活性在第1天显著升高(p<0.05)后下降。研究结果显示，低氧胁迫对军曹鱼幼鱼造成一定程度的氧化损伤，抑制其生长并使血清生化和非特异性免疫相关指标发生显著变化，表明军曹鱼机体物质代谢和免疫系统受到影响。
- 军曹鱼 /
- 低氧 /
- 生长 /
- 生化指标 /
- 非特异性免疫
Abstract: In order to study the effect of hypoxia stress on serum of biochemical and non-specific immune indexes of the juvenile cobia(Rachycentron canadum), the fish with body weight of (50.44±2.78) g were treated at dissolved oxygen content of (3.15±0.21) mg/L for 4 weeks. The samples were taken on 1 d, 7 d, 14 d and 28 d respectively for determination of relevant indexes and results showed that: (1) the growth of juvenile cobia was inhibited by hypoxia, the weight gain rate and specific growth rate were significantly lower than the control level (p<0.05); (2) the indexes of liver function showed that activities of aspartate aminotransferase and alanine aminotransferase significantly increased (p<0.01) on 7 d, 14 d, 28 d and 14 d, 28 d respectively, while the content of total protein and albumin significantly increased (p<0.05) on 14 d and 28 d than that of the control group; (3) the content of blood glucose significantly increased (p<0.05) on 1 d, 14 d and 28 d, while the triglyceride significantly increased (p<0.01) on 14 d and 28 d. The total cholesterol content significantly decreased (p<0.05) on 1 d, then continue to increase; (4) the serum ion content changed with different trend, in which sodium ion and chloride ion significantly increased (p<0.01) on 14 d and 28 d. Potassium content increased continuously and was significantly higher (p<0.05) on 7 d, 14 d and 28 d than control level, while calcium content continued to decrease and there was a significant difference (p<0.05) on 14 d and 28 d; (5) activitie of superoxide dismutase and content of malondialdehyde continued to increase and were significantly higher (p<0.01) than those in the control group on 7 d, 14 d, 28 d and 14 d, 28 d respectively. Activities of catalase showed significantly “decreased-increased-decreased” patterns. Activitie of lysozyme significantly increased (p<0.05) on 1 d and then continued to decrease. These results showed that hypoxia stress caused oxidative damage to the juvenile cobia, inhibited its growth and led to significant change in serum biochemical and non-specific immune indexes, which indicating that the nutrient utilization and immune system of cobia were affected.
- Rachycentron canadum /
- hypoxia /
- growth /
- biochemical indexes /
- non-specific immune

HTML全文

图 1 低氧胁迫对军曹鱼幼鱼特定生长率（a）、增重率（b）的影响

*表示差异显著（p<0.05），**表示差异极显著（p<0.01）

Fig. 1 Effects of hypoxia on the specific growth rate (a) and weight gain rate (b) in the serum of juvenile cobia (Rachycentron canadum)

*Shows significant difference (p<0.05), **shows extremely significant difference (p<0.01)

下载: 全尺寸图片幻灯片

图 2 低氧胁迫对军曹鱼幼鱼血清谷草转氨酶（a）、谷丙转氨酶（b）活性和总蛋白（c）、白蛋白（d）含量的影响

*表示差异显著（p<0.05），**表示差异极显著（p<0.01）

Fig. 2 Effects of hypoxia on the activities of aspartate aminotransferase (a), alanine aminotransferase (b), and the contents of total protein (c) and albumin (d) in the serum of juvenile cobia (Rachycentron canadum)

*Shows significant difference (p<0.05), **shows extremely significant difference (p<0.01)

下载: 全尺寸图片幻灯片

图 3 低氧胁迫对军曹鱼幼鱼血清葡萄糖（a）、总胆固醇（b）和甘油三酯（c）含量的影响

*表示差异显著（p<0.05），**表示差异极显著（p<0.01）

Fig. 3 Effects of hypoxia on the contents of glucose (a), total cholesterol (b), and triglyceride (c) in the serum of juvenile cobia (Rachycentron canadum)

*Shows significant difference (p<0.05), **shows extremely significant difference (p<0.01)

下载: 全尺寸图片幻灯片

图 4 低氧胁迫对军曹鱼幼鱼血清钠离子（a）、钾离子（b）、氯离子（c）和钙离子（d）含量的影响

*表示差异显著（p<0.05），**表示差异极显著（p<0.01）

Fig. 4 Effects of hypoxia on the contents of sodium ion (a), potassium ion (b), chloride ion (c) and calcium ion (d) in the serum of juvenile cobia (Rachycentron canadum)

*Shows significant difference (p<0.05), **shows extremely significant difference (p<0.01)

下载: 全尺寸图片幻灯片

图 5 低氧胁迫对军曹鱼幼鱼血清超氧化物歧化酶（a）、过氧化氢酶（b）、溶菌酶（d）活性和丙二醛（c）含量的影响

*表示差异显著（p<0.05），**表示差异极显著（p<0.01）

Fig. 5 Effects of hypoxia on the activities of superoxide dismutase (a), catalase (b), lysozyme (d) and the contents of malondialdehyde (c) in the serum of juvenile cobia (Rachycentron canadum)

*Shows significant difference (p<0.05), **shows extremely significant difference (p<0.01)

下载: 全尺寸图片幻灯片

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