"鲆优1号"牙鲆和亲本群体的表型及遗传性状变异分析

田永胜; 齐文山; 姜静; 王磊; 张英平; 刘万军; 陈红林; 陈松林

"鲆优1号"牙鲆和亲本群体的表型及遗传性状变异分析

1.
中国水产科学研究院黄海水产研究所, 海洋渔业可持续发展重点实验室, 山东青岛 266071
2.
中国水产科学研究院黄海水产研究所, 海洋渔业可持续发展重点实验室, 山东青岛 266071;上海海洋大学水产与生命学院, 上海 201306
3.
中国水产科学研究院黄海水产研究所, 海洋渔业可持续发展重点实验室, 山东青岛 266071;中国海洋大学海洋生命学院, 山东青岛 266003

基金项目: “863”高技术研究发展计划（2012AA10A408）；山东省泰山学者建设工程专项资助。

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出版历程
- 收稿日期: 2013-04-18

Analysis of phenotype and genetic variation of on “Flounder No.1” Paralichthys Olivaceus and their parental populations

1.
Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China
2.
Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
3.
Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;School of Marine life Sciences, 0cean University of China, Qingdao 266003, China

摘要

摘要: 牙鲆是我国及大西洋沿岸天然捕捞和养殖的主导品种，对其遗传资源保护及养殖新品种开发具有重要的意义。本文对人工培育的牙鲆养殖新品种“鲆优1号”（ZJ）、其父本韩国牙鲆群体（KS）、母本F0750家系（JX）的18个表型性状进行测量，利用SSPS程序进行方差分析和多重比较，发现体长/体宽，头长/眼径，头长/眼间距、眼径/眼间距，侧线鳞和尾鳍条6个性状与父本或母本具有显著性差异（p<0.05），可做为鉴别“鲆优1号”的标志性表型性状。“鲆优1号”与母本无显著差异（p>0.05）性状占50%。与父本无显著性差异（p>0.05）性状占33.33%。筛选了24个多态性微卫星引物对KS、JX、ZJ和FJ的遗传多样性进行分析，总共扩增出143个等位基因。 4个群体的平均等位基因数（N_a）大于平均有效等位基因数（N_ae），平均观测杂合度大于平均期望杂合度，多态信息含量（PIC_a）分析显示：KS>JX>FJ>ZJ，说明人工选育导致了遗传杂合度的降低。24个微卫星位点在4个牙鲆群体中的遗传偏离指数（D）在-0.759 7到0.188 4之间，在KS、JX、ZJ和FJ群体中分别有10、16、11和17个位点显著（p<0.05）或极显著（p<0.01）偏离Hardy-Weinberg平衡。24个基因位点的遗传分化系数（F_st）在0.076 7~0.258 6之间，平均为0.146 8，表明14.68%的变异来自种群间，85.32%的变异来自种群内。Neis遗传距离显著，KS和FJ遗传距离最近（0.282 5），遗传相似系数最大（0.753 9）；ZJ与JX遗传距离最大（0.420 5），遗传相似系数最小（0.656 7）。本文研究表明：“鲆优1号”具有明显的表型和遗传特征，与父母本及反交群体比较发生了一定程度的种内和种群间遗传分化，为“鲆优1号”的种质鉴别提供了理论依据。
- 牙鲆 /
- 鲆优1号 /
- 表型性状 /
- 卫微星标记 /
- 遗传多样性
Abstract: Japanese flounder (Paralichthys olivaceus) is one of the most popularspecies for fishing and breeding in China and the Atlantic coast,so it is of great importance for the protection of genetic resources and the development of new species. In present study,18 phenotypic traits of "Flounder No.1" (ZJ),a newly breeding species of Japanese flounder,as well as its male (the Korean Japanese flounder stock,KS) and female parents (F0750 family,JX),were measured. The data were compared by ANOVA and SPSS software. The results showed that there were significant differences (p<0.05) between "flounder No.1" and its parents regarding 6 traits,e.g. body length/body width,head length/eye diameter,head length/eye spacing,eye diameter/eye spacing,lateral line scales and caudal fin ray which can be used as typical characters for identification of "Flounder No.1". In "Flounder No.1",50% of the traits showed no significant differences (p>0.05) bcompared to its female parent,while the value decreased to 33.3% between "flounder No.1" and its male parent. A total of 24 microsatellite primers were designed to screen the genetic diversity of KS,JX,ZJ and FJ (reciprocal cross offspring),and 143 alleles were identified. For four populations,KS,JX,ZJ and FJ,their mean allelic number (N_a) was higher than the effective mean number of alleles (N_ae),the mean heterozygosity was higher than the predicted value. The polymorphism information content (PIC_a) showed that KS>JX>FJ>ZJ,indicating that the artificial breeding caused the reduction of the genetic heterozygosity. Among the four populations,the genetic deviation index (D) of 24 above mentioned microsatellite loci ranged from-0.759 7 to 0.188 4. And in KS,JX,ZJ and FJ,10,16,11 and 17 loci were significantly (p<0.05) or extremely (p<0.01) deviated from Hardy-Weinberg equilibrium,respectively. The genetic-differentiation-index (F_st) of 24 loci ranged from 0.076 7 to 0.258 6,with the mean value 0.1468,which suggests that 14.68% of the variations result from the interspecies,while 85.32% are intraspecific variations. Furthermore,there was significant difference for Neis genetic distance among four populations. The nearest genetic distance occured between KS and FJ (0.282 5),togehter with the highest genetic similarity coefficient (0.753 9). While the furthest (0.420 5) one was found between ZJ and JX with the lowest (0.656 7) genetic similarity coefficient. In this study,the observation indicated: "flounder No.1" has the obvious phenotypic and genetic characteristics,shows a certain degree of genetic differentiation in intraspecies and interspecies in comparison to its parents and reciprocal cross offspring,which provides the theoretical basis for the germplasm identification of "Flounder No.1".
- Paralichthys Olivaceus /
- “Flounder No.1” /
- phenotypic traits /
- microsatellite markers /
- genetic diversity

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参考文献(25)

杨正勇,王春晓. 全球视野下中国鲆鲽类养殖业的发展[J]. 中国渔业经济,2009,6(27): 115-127.

陈松林,田永胜,徐田军,等. 牙鲆抗病群体和家系的建立及其生长和抗病性能初步测定[J]. 水产学报,2008,32(5):665-674.

田永胜,徐田军,陈松林,等. 三个牙鲆育种群体亲本效应及遗传参数估计[J]. 海洋学报,2009,31(6):119-129.

陈松林,田永胜. 全国水产技术推广总站-2010水产新品种推广指南[M]. 北京:中国农业出版社,2011:242-268.

刘永新,刘海金. 牙鲆不同家系早期形态性状差异比较[J].东北农业大学学报,2008,39(8):82-88.

赵莹莹,朱晓琛,孙效文. 虹鳟6 个养殖群体遗传多样性的微卫星分析[J]. 遗传,2006,28(8): 956-962.

全迎春,孙效文,梁利群. 应用微卫星多态分析四个鲤鱼群体的遗传多样性[J]. 动物学研究,2005,26(6) : 595-602.

Kang J H,Kim W J,Lee W J. Genetic linkage map of olive flounder,Paralichthys olivaceus[J]. Int J Biol Sci,2008,4(3): 143-149.

Song W T,Pang R Y,Niu Y Z,et al. Construction of high-density genetic linkage maps and mapping of growth-related quantitative trail loci in the Japanese flounder (Paralichthys olivaceus)[J]. Plos One,2012,7(11): e50404.

Fuji K,Hasegawa O,Honda K,et al. Marker-assisted breeding of a lymphocystis disease-resistant Japanese flounder (Paralichthys olivaceus) [J]. Aquaculture,2007,272: 291-295.

田永胜,陈松林,严安生. 牙鲆胚胎玻璃化冷冻技术研究[J]. 高技术通讯,2005,15 (3): 105-110.

李思忠,王惠民. 中国动物志:硬骨鱼纲-鲽形目[M]. 北京:科学出版社,1995:116-123.

王新安,马爱军,侯仕营,等. 大菱鲆4个不同地理群体生长性能的比较[J]. 渔业科学进展,2010,31(1): 34-39.

于飞,张庆文,孔杰,等. 大菱鲆测量性状对体重的影响效果分析[J].海洋水产研究,2008,29(60):33-40.

夏德全,曹萤,杨弘,等.罗非鱼杂交F 代与亲本的遗传关系及其杂种优势的利用[J]. 水产科学,1999,6(4):29-32.

杜诚,卢迈新,叶星,等. 5个罗非鱼群体的遗传分析与分子标记[J]. 大连海洋学院学报,2005,20(1):25-28.

刘海金,朱晓琛,孙效文,等. 牙鲆5 个养殖群体的遗传多样性分析[J].中国水产科学,2008,15(1):30-38.

刘云国,陈松林,李八方,等. 牙鲆选择性养殖群体遗传结构的微卫星分析[J].高技术通讯,2006,16(1):94-99.

Kim W J,Kim K K,Han H S,et al. Population structure of the olive flounder (Paralichthys olivaceus) in Korea inferred from microsatellite marker analysis[J]. J Fish Biol,2010,76: 1958-1971.

Sekino M,Hara M. Application of microsatellite markers to population genetics studies of Japanese flounder Paralichthys olivaceus[J]. Mar Biotechnol (NY),2001,3: 572-589.

Li D,Kang D,Yin Q,et al. Microsatellite DNA marker analysis of genetic diversity in wild common carp (Cyprinus carpio L.) populations[J]. J Genet Genomics,2007,34: 984-993.

Nei M,Maruyama T,Chakraborty R. The bottleneck effect and genetic variability in populations[J]. Evolution,1975,29(1): 1-10.

Wang L,Zhang L D,Wan Y M,et al. Isolation of microsatellite markers from Paralichthys olivaceus and its application in genetic structure analysis[J]. Yi Chuan,2010,32: 1057-1064.

李鹏飞,刘萍,柳学周,等. 漠斑牙鲆引进种群同工酶的遗传多态性分析[J]. 中国水产科学,2006,13(1): 13-19.

Suci A,Uthairat N N,Worawut K. Study of genetic diversity of orange spotted grouper,Epinephelus coioides from Thailand and Indonesia using microsatellite markers[J]. Mar Biotechnol,2005,8: 17-26.

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