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15N示踪法测定海洋反硝化速率中29N2浓度的准确定量

曾健,  陈敏,  郑敏芳,  邱雨生

文章导航 > 海洋学报  > 2014 >  36(6): 10-17
曾健, 陈敏, 郑敏芳, 邱雨生. 15N示踪法测定海洋反硝化速率中29N2浓度的准确定量[J]. 海洋学报, 2014, 36(6): 10-17.
引用本文: 曾健, 陈敏, 郑敏芳, 邱雨生. 15N示踪法测定海洋反硝化速率中29N2浓度的准确定量[J]. 海洋学报, 2014, 36(6): 10-17.
shu
Zeng Jian, Chen Min, Zheng Minfang, Qiu Yusheng. Accurately quantification of 29N2 concentration in 15N tracer derived marine denitrificaiton rates[J]. Haiyang Xuebao, 2014, 36(6): 10-17.
Citation: Zeng Jian, Chen Min, Zheng Minfang, Qiu Yusheng. Accurately quantification of 29N2 concentration in 15N tracer derived marine denitrificaiton rates[J]. Haiyang Xuebao, 2014, 36(6): 10-17.
shu

15N示踪法测定海洋反硝化速率中29N2浓度的准确定量

  • 1.

    厦门大学 海洋与地球学院, 福建 厦门 361005

  • 2.

    厦门大学 海洋与地球学院, 福建 厦门 361005;近海海洋环境科学国家重点实验室, 福建 厦门 361005

基金项目: 国家自然科学基金杰出青年基金项目（41125020）；国际海域资源调查与开发“十二五”课题（DY125-13-E-01）；科技基础性工作专项（2008FY110100）。

Accurately quantification of 29N2 concentration in 15N tracer derived marine denitrificaiton rates

  • 1.

    College of Ocean and Earth Sciences, Xiamen University, Xiamen 361005, China

  • 2.

    College of Ocean and Earth Sciences, Xiamen University, Xiamen 361005, China;State Key Laboratory of Marine Environment Science, Xiamen University, Xiamen 361005, China

    摘要
  • 摘要: 利用15N示踪法实测南海水体反硝化速率的研究发现，培养水样在长时间密闭放置过程中也会受到外界空气的污染，且其29N2/28N2比值恒定为0.007 35。根据空气背景中29N2/28N2比值恒定的特征，提出基于质量平衡关系校正空气N2污染的方法，通过将样品实测29N2浓度扣除由外界空气贡献的29N2浓度，可获得由生物反硝化作用所产生的29N2准确浓度，进而可计算出准确的反硝化速率。经空气29N2背景校正后，29N2浓度的偏差明显小于未经校正的结果，且29N2浓度与培养时间之间的线性相关性显著加强，凸显出空气29N2背景校正是获取准确反硝化速率的关键。鉴于15N示踪法已被广泛应用于海洋水体与沉积物反硝化速率的测定中，所提出的空气29N2背景校正方法具有重要的意义。
    Abstract: 15N tracer technique was widely applicated in measuring water column denitrification rate in water samples,however,the sealed samples could be contaminated by air during a long time storage. Our study suggested that N2 in air has a constant 29N2/28N2 ratio of 0.007 35. According to this feature,a mass-balance calculation was proposed to calibrate the interference from air background. It helps obtain more accurately 29N2 concentrations produced by biological activity and further the denitrification rates. After the calibration,the deviations of 29N2 concentrations were much less than those of the raw data,and the linear correlation between 29N2 concentration and the incubation time was more significant. It is important to calibrate extra 29N2 signals from air contamination for accurately quantification of denitrification rate,considering the 15N technique has been widely used in pelagic and beneath denitrification rate measurements.
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