旋链角毛藻对中肋骨条藻化感作用的影响因素

张议文; 王江涛; 谭丽菊

旋链角毛藻对中肋骨条藻化感作用的影响因素

中国海洋大学海洋化学理论与工程技术教育部重点实验室,山东青岛,266100

基金项目: 国家自然科学基金(41076065)；"973"项目(2010CB428701)。

计量
- 文章访问数: 1533
- HTML全文浏览量: 24
- PDF下载量: 1615
- 被引次数: 0
出版历程
- 收稿日期: 2012-09-06

The study on the influencing factors of Chaetoceros curvisetus allelopathic effect on Skeletonema costatum and the primary research on the properties of Chaetoceros curvisetus allelochemical

Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China

摘要

摘要: 在已知旋链角毛藻对中肋骨条藻具有化感作用的前提下，进行了温度、生长时期、光照、细菌等因素对旋链角毛藻化感作用的影响、化感物质的萃取及粗提物对中肋骨条藻的化感效应检验等实验，研究了旋链角毛藻化感物质的性质、降解特点，并初步测定了化感物质的结构。结果表明：旋链角毛藻化感作用与生长时期有关，指数期滤液化感作用强于衰亡期;旋链角毛藻的化感物质在50℃以下保持稳定，光和细菌均引起旋链角毛藻化感物质的降解，且细菌降解强于光降解;旋链角毛藻的乙酸乙酯萃取物具有明显的化感活性，该萃取物在255～260 nm处有特征吸收峰。综合以上结果，推测旋链角毛藻化感物质可能为分解温度在50℃以上的易被光和细菌降解的苯衍生物，从极性上看较易溶于乙酸乙酯。
- 化感物质 /
- 旋链角毛藻Chaetoceroscurvisetus /
- 中肋骨条藻Skeletonemacostatum
Abstract: On the basis of the fact that Chaetoceros curvisetus showed allelopathic effect on Skeletonema costatum, a series of experiments, including algal cultures and compounds extraction, were carried out to research the properties, influence factors, degradation characteristics and structure of C. curvisetus allelochemical. The results indicated that allelopathic effect of C. curvisetus was related to the growth phase, and the C. curvisetus filtrates in exponential phase caused the inhibition on the growth of S. costatum than that of declining phase filtrates. Chaetoceros curvisetus allelochemical remain stable under 50℃ temperature and degraded as time went on attributed on light and bacteria, and bacteria contributed more. Furthermore, the ethyl acetate extraction of C. curvisetus filtrate showed significant allelopathic effect and the wavelength of characteristic absorption for the growth inhibitor was 255-260 nm. Consequently, the effect ingredient was dissolved by ethyl acetate. In addition, the allelochemical of C. curvisetus was supported to be benzene derivatives whose decomposition temperature was more than 50℃ and could be degraded by light and bacteria.
- allelochemical /
- Chaetoceros curvisetus /
- Skeletonema costatum

HTML全文

参考文献(26)

International Allelopathy Society 1996. Constitution. Drawn up during First World Congress on Allelopathy: A Science for the Future. Cadiz, Spain, 1996. Available at: http://www.ias.uca.es/ bylaws.htm#CONST1.

Gross E M. Allelopathy of aquatic autotrphs[J]. Critical Reviews in Plant Sciences. 2003, 22:313-339.

Granéli E, Weberg M, Paulo S. Harmful algal blooms of allelopathic microalgal species: The role of eutrophication[J]. Harmful Algae. 2008, 8:94-102.

Liu J S, Vanrijssel M, Yang W D, et al. Negative effects of Phaeocystis globosa on microalgae[J]. Chinese Journal of Oceanology and Limnology. 2010, 28(4):911-916.

Yamasaki Y, Ohmichi Y, Shikata T, et al. Species-specific allelopathic effects of the diatom Skeletonema costatum[J]. Thalassas, 2010, 27:21-32.

王江涛,李慧,曹婧,等. 化感作用对中肋骨条藻与东海原甲藻竞争演替的影响[J]. 海洋学报, 2012, 34(1): 1-9.

张议文, 王江涛. 旋链角毛藻和中肋骨条藻间的他感作用[J]. 海洋环境科学, 2012, 31(4): 474-478.

祝陈坚, 韩秀荣, 谭丽菊,等. 海水分析化学实验[M]. 青岛: 中国海洋大学出版社, 2006,40-63.

Lu H M, Liao X J, Yang Y F, et al. Effects of Extracts from Gracilaria lemaneiform on Microalgae[J]. Ecological Science, 2008, 27(5): 424-426.

梅长林, 范金城. 数据分析方法[M]. 北京: 高等教育出版社, 2006(2):83-85.

Wang Y, Yu Z M, Song X X,et al. Interactions between the bloom-forming dinoflagellates Prorocentrum donghaiense and Alexandrium tamarense in laboratory cultures[J]. J S Res, 2006, 56:17-26.

Mykestad S. Release of extracellular products by phytoplankton with special emphasis on polysaccharides[J]. The Science of the Total Environment, 1995, 165: 155-164.

Moran M, Sheldon W. Carbon loss and optical property changes during long-term photochemical andbiological degradation of estuarine dissolved organic matter[J]. Limnol Oceanogr, 2000, 45(6):1254-1264.

Uronen P, Kuuppo P, Legrand C, et al. Allelopathic effects of toxic haptophyte Prymnesium parvum lead to release of dissolved organic carbon and increase in bacterial biomass[J]. Microbial Ecology,2007, 54:183-193.

Tillmann U, Alpermann T, John U, et al. Allelochemical interactions and short-term effects of the dinoflagellate Alexandrium on selected photoautotrophic and heterotrophic protists[J]. Harmful Algae, 2008, 7:52-64.

Weissbach A, Rudstrom M, Olofsson M, et al. Phytoplankton allelochemical interactions change microbial food web dynamics[J]. Limnol Oceanogr, 2011,56(3):899-909.

王江涛, 尹晓楠, 宋茜. 几种抗生素的抑菌效果及其对中肋骨条藻生长的影响[J].中国海洋大学学报, 2008, 38(3):468-472.

Obernosterer I, Benner R. Competition between biological and photochemical processes in the mineralization of dissolved organic carbon[J]. Limnol Oceanogr, 2004, 49(1):117-124.

丁慧, 蔺存国, 郑纪勇,等. 孔石莼提取物对海洋硅藻生长的影响[J]. 海洋环境科学, 2009, 28(6): 620-622.

冀晓青.几株典型赤潮藻化感作用及化感物质研究 . 青岛:中国海洋大学,2010.

别聪聪, 李锋民, 李媛媛. 六种大型藻浸提液对中肋骨条藻的抑制及活性成分分离[J]. 中国海洋大学学报, 2011, 41(7): 107-112.

邢其毅,裴伟伟,徐瑞秋,等. 基础有机化学[M]. 北京: 高等教育出版社,2005:156-168.

孙颖颖, 王长海, 陈静. 球等鞭金藻细胞生长抑制物的分离纯化[J]. 光谱学与光谱分析, 2008, 28(2): 430-435.

鲜啟鸣,陈海东,邹惠仙,等. 沉水植物中挥发性物质对铜绿微囊藻的化感作用[J]. 生态学报, 2006, 26(11): 3549-3554.

Zhang T, Zheng C, Hu W, et al. The allelopathy and allelopathic mechanism of phenolic acids on toxic Microcystis aeruginosa[J]. J Appl Phycol, 2010, 22: 71-77.

郑春艳, 张哲, 胡威, 等. 三种化感物质对水华混合藻类以及多刺裸腹蚤的毒性作用[J].中国环境科, 2010, 30(5): 710-715.

施引文献

资源附件(0)

访问统计