旋链角毛藻对中肋骨条藻化感作用的影响因素

张议文; 王江涛; 谭丽菊

旋链角毛藻对中肋骨条藻化感作用的影响因素

中国海洋大学海洋化学理论与工程技术教育部重点实验室,山东青岛,266100

基金项目: 国家自然科学基金(41076065)；"973"项目(2010CB428701)。

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出版历程
- 收稿日期: 2012-09-06

The study on the influencing factors of Chaetoceros curvisetus allelopathic effect on Skeletonema costatum and the primary research on the properties of Chaetoceros curvisetus allelochemical

Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China

摘要

摘要: 在已知旋链角毛藻对中肋骨条藻具有化感作用的前提下，进行了温度、生长时期、光照、细菌等因素对旋链角毛藻化感作用的影响、化感物质的萃取及粗提物对中肋骨条藻的化感效应检验等实验，研究了旋链角毛藻化感物质的性质、降解特点，并初步测定了化感物质的结构。结果表明：旋链角毛藻化感作用与生长时期有关，指数期滤液化感作用强于衰亡期;旋链角毛藻的化感物质在50℃以下保持稳定，光和细菌均引起旋链角毛藻化感物质的降解，且细菌降解强于光降解;旋链角毛藻的乙酸乙酯萃取物具有明显的化感活性，该萃取物在255～260 nm处有特征吸收峰。综合以上结果，推测旋链角毛藻化感物质可能为分解温度在50℃以上的易被光和细菌降解的苯衍生物，从极性上看较易溶于乙酸乙酯。
- 化感物质 /
- 旋链角毛藻Chaetoceroscurvisetus /
- 中肋骨条藻Skeletonemacostatum
Abstract: On the basis of the fact that Chaetoceros curvisetus showed allelopathic effect on Skeletonema costatum, a series of experiments, including algal cultures and compounds extraction, were carried out to research the properties, influence factors, degradation characteristics and structure of C. curvisetus allelochemical. The results indicated that allelopathic effect of C. curvisetus was related to the growth phase, and the C. curvisetus filtrates in exponential phase caused the inhibition on the growth of S. costatum than that of declining phase filtrates. Chaetoceros curvisetus allelochemical remain stable under 50℃ temperature and degraded as time went on attributed on light and bacteria, and bacteria contributed more. Furthermore, the ethyl acetate extraction of C. curvisetus filtrate showed significant allelopathic effect and the wavelength of characteristic absorption for the growth inhibitor was 255-260 nm. Consequently, the effect ingredient was dissolved by ethyl acetate. In addition, the allelochemical of C. curvisetus was supported to be benzene derivatives whose decomposition temperature was more than 50℃ and could be degraded by light and bacteria.
- allelochemical /
- Chaetoceros curvisetus /
- Skeletonema costatum

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