我国近海中度嗜盐菌的分离筛选及其产酶多样性分析

产竹华; 刘洋; 王昭凯; 钟添华; 曾润颖

我国近海中度嗜盐菌的分离筛选及其产酶多样性分析

国家海洋局第三海洋研究所海洋生物遗传资源重点实验室,福建厦门 361005

基金项目: 国家海洋局第三海洋研究所基本科研业务费专项资金资助项目(海三科2009043)；国家"863"计划(2007AA091407)；福建省自然科学基金资助项目(2012J01174)。

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- 收稿日期: 2013-01-27

Isolation, screening and enzyme diversity of moderately halophilic bacteria in Chinese coastal waters

Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China

摘要

摘要: 为了解我国近海中度嗜盐菌系统发育多样性及产酶特性，从天津、山东、江苏、福建、海南等地近海采集样品为研究对象，分离筛选得到108株中度嗜盐菌，其中有26株至少产一种酶。通过对其形态特征、生理生化、16S rRNA基因序列及系统进化树进行分析，将26株菌鉴定为细菌域的Halomonas、Idiomarina、Virgibacillus、Pontibacillus、Oceanobacillus、Halobacillus和Marinilactibacillus属的菌株。它们与相应属的模式菌株16S rRNA基因序列相似性在97%～100%不等，其中有些菌株可能代表不同的分类单元。底物特异性试验表明，分离的26株中度嗜盐菌13株产蛋白酶，19株产淀粉酶，13株产酯酶，4株产纤维素酶。其中6株产3种酶，11株产2种酶。研究结果表明中度嗜盐菌具有系统发育和产酶多样性，同时蕴藏着较多新的微生物类群，为嗜盐微生物资源应用开发提供了参考。
- 中度嗜盐菌 /
- 系统进化分析 /
- 16SrRNA基因序列 /
- 产酶多样性
Abstract: To investigate phylogenetic and enzyme production diversity of moderately halophilic bacteria, we got the samples from China's offshore of Tianjing, Shandong, Jiangsu, Fujian and Hainan. 108 moderately halophilic strains were isolated, 26 of which could produce at least one type of enzymes. According to physiological and biochemical characteristics, 16S rRNA sequences and phylogenetic analysis, the 26 moderately halophilic strains belonged to genera Halomonas, Idiomarina, Virgibacillus, Pontibacillus, Oceanobacillus, Halobacillus or Marinilactibacillus separately. The sequences had the similarity(97%-100%) to the type strains of each genera. Some moderately halophilic bacteria might be a novel species. The substrates specific experiment indicated that there were 13 moderately halophilic strains producing protease, 19 strains producing amylase, 13 strains producing esterase, 4 strains producing cellulose. And there were 6 strains producing three enzymes and 11 strains producing two enzymes simultaneously. The research indicated that there was not only abundant phylogenetic and enzyme diversity of moderately halophilic bacteria, but also some unknown bacteria groups existed in China's offshore, which provided references for reasonable development and utilization of halophilic microbial resources.
- moderately halophilic bacteria /
- phylogenetic analysis /
- 16S rRNA sequences /
- enzyme production diversity

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