全国海水浴场及经济贝类中腺病毒的分布

明红霞; 樊景凤; 梁玉波; 关道明

全国海水浴场及经济贝类中腺病毒的分布

国家海洋局国家海洋环境监测中心,辽宁大连 116023

基金项目: 海洋公益性行业科研专项(201105007)；国家海洋局近岸海域生态环境重点实验室开放研究基金(201201)。

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出版历程
- 收稿日期: 2013-01-11

The distribution of adenovirus in bathing beach and commercial shellfish of China

National Marine Environmental Monitoring Center,State Oceanic Administration, Dalian 116023,China

摘要

摘要: 为分析全国海水浴场、贝类增养殖区人类肠道腺病毒的污染状况，采用超滤浓缩方法，TaqMan探针实时定量PCR技术对其进行了定量。结果表明，全国 10个海水浴场表层海水中腺病毒的阳性检出率为35%，含量为1.1×10⁷～2.8×10⁷ copies/L。在离岸100 m和1 000 m处腺病毒的分布无显著差别(p>0.05);在调查的162份贝类样品中，腺病毒的阳性检出率为11.7%，含量为2.4×10⁴～3.1×10⁵ copies/g。6类经济贝类中牡蛎带病毒量最高。由此可见，全国范围内海水浴场表层海水和主要增养殖区经济贝类中腺病毒污染比较普遍，尤其以山东、辽宁和浙江三省最为严重，给洗浴者和消费者健康造成潜在威胁。人类肠道腺病毒可以很好地指示粪便污染，调查其在海水浴场和经济贝类中的分布，可以帮助公众预警胃肠道病毒的暴发流行，本研究为开展全国腺病毒的环境风险评估和分子流行病学调查奠定了良好的基础。
- 人类肠道腺病毒 /
- 表层海水 /
- 经济贝类 /
- TaqMan探针实时定量PCR技术
Abstract: In order to analyze the contamination of human enteric viruses in bathing beach and economic shellfish along the primarily commercial shellfish-breeding areas in coastal cities, we chose adenovirus as the object of this study. Virus was concentrated by efficient ultra-centrifuge method, then TaqMan real-time PCR was employed to quantify its concentration. The percentage of positive samples for adenovirus was 35%in ten bathing beaches of China. The concentration was 1.1×10⁷-2.8×10⁷ copies/L. The viral incidence in the water samples collected from 100 meters off the seashore was found not to be correlated with those from 1 000 meters out (p>0.05). The percentage of positive samples for adenovirus was 11.7% in 162 samples, the concentration ranged from 2.4×10⁴ to 3.1×10⁵ copies/g. During six chosen commercial shellfish, Oyster apparently had the strongest ability to carry adenovirus. Hence, the contamination of seawater and shellfish by human adenovirus was relatively common. The fecal contamination in Shandong Province, Liaoning Province and Zhejiang Province were more severe, indicating a potential public health threat for bathers and consumers. Human enteric adenovirus was a good indicator to present the fecal pollution. Therefore, investigating its distribution from bathing beach and commercial shellfish may help to alert community health to enteric viruses that are in circulation in the population. This study laid a good foundation for carrying out the risk assessment and molecular epidemiological investigation of adenovirus in the whole China.
- human enteric adenovirus /
- surface seawater /
- commercial shellfish /
- TaqMan real-time PCR

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参考文献(31)

Lopman B A, Reacher M H, Van Duijinhoven Y, et al. Viral gastroenteritis outbreaks in Europe, 1995-2000[J]. Emerg Infect Dis, 2003, 9: 90-96.

Tang Y W, Wang J X, Xu Z Y, et al. A serologically confirmed case-control study of a large outbreak of hepatitis A in China associated with consumption of clams[J]. Epidemiol Infect, 1991, 107: 651-658.

Jurzik L, Hamza I A, Puchert W, et al. Chemical and microbiological parameters as possible indicators for human enteric viruses in surface water[J]. Int J Hyg Environ Heal, 2010, 213: 210-216.

Rigotto C, Hanley K, Rochelle P A, et al. Survival of adenovirus types 2 and 41 in surface and ground waters measured by a plaque assay[J]. Environ Sci Technol, 2011, 45: 4145-4150.

Hundesa A, Maluquer D E M, Albinana G C, et al. Development of a qPCR assay for the quantification of porcine adenoviruses as an MST tool for swine fecal contamination in the environment[J]. J Virol Methods, 2009, 158: 130-135.

Silva H D, García-Zapata M T A, Anunciao C E. Why the Use of Adenoviruses as Water Quality Virologic Marker ？ [J]. Food Environ Virol, 2011, 3:138-140.

Cromeans T L, Kahler A M, Hill V R. Inactivation of adenoviruses, enteroviruses, and murine norovirus in water by free chlorine and monochloramine[J]. Appl Environ Microbiol. 2010, 76 (4): 1028-1033.

U.S. Environmental Protection Agency-USEPA. Drinking Water Contaminant Candidate List 3-Final . Federal Register, 2009, 51856. Washington, DC.

Formiga C M, Allard A K, Conden-Hansson A C, et al. Evaluation of potential indicators of viral contamination in shellfish and their applicability to diverse geographical areas[J]. Appl Environ Microbiol, 2003, 69(3): 1556-1563.

Jiang S C, Noble R, Chu W. Human adenovirus in water: Occurrence and health implication: A critical review[J]. Environ Sci Technol, 2006, 40(23):7132-7140.

He J W, Jiang S. Quantification of enterococci and human adenoviruses in environmental samples by real-time PCR[J]. Appl Environ Microbiol, 2005, 71: 2250-2255.

Haramoto E, Katayama H, Oguma K, et al. Application of cationcoated filter method to detection of noroviruses, enteroviruses, adenovirses, and Torque Teno viruses in the Tamagawa River in Japan[J]. Appl Environ Microbiol, 2005,71(5): 2403-2411.

Costafreda M I, Bosch A, Pintó R M. Development, Evaluation, and Standardization of a real-time TaqMan Reverse Transcription-PCR Assay for Quantification of Hepatitis A Virus in Clinical and Shellfish Samples[J]. Appl Microbiol, 2006, 72(6)3846-3855.

Aslan A, Xagoraraki I, Simmons F J, et al. Occurrence of adenovirus and other enteric viruses in limited-contact fresh water recreational areas and bathing waters[J]. J Appl Microbiol, 2011, 111 (5): 1250-1261.

Haramoto E, Katayama H, Oguma K, et al. Quantitative analysis of human enteric adenoviruses in aquatic environments[J]. J Appl Microbiol, 2007, 103(6): 2153-2159.

Albinana G N, Clemente C P, Bofill M S, et al. Distribution of human polyomaviruses, adenoviruses, and hepatitis E virus in the environment and in a drinking-water treatment plant[J]. Environ Sci Technol, 2006, 40(23): 7416-7422.

明红霞,樊景凤,吴利军,等. 四种人肠道病毒在大连金石滩浴场中的分布[J]. 海洋环境科学,2008,27(6):584-587.

Ming H X, Fan J F, Wu L J, et al. Prevalance of human enteric viruses and a potential indicator of contamination in shellfish in China[J]. J Food Fafety, 2013, 33(2):209-214.

Fan J F, Ming H X, Wu L J, et al. Investigation of fecal coliform and typical enteric virus in representative beaches of China[J]. Marine science bulletin, 2011, 13(2): 72-82.

Jothikumar N, Cromeans T L, Sobsey M D, et al. Development and evaluation of a broadly reactive TaqMan assay for rapid detection of hepatitis A virus[J]. Appl Environ Microbiol, 2005,71: 3359-3363.

Kageyama T, Kojima S, Shinohara M, et al. Broadly reactive and highly sensitive assay for Norwalk-like viruses based on real-time quantitative reverse transcription-PCR[J]. J Clin Microbiol, 2003, 41(4):1548-1557.

Hernroth B. The persistence of infectious adenovirus (type 35) in mussels (Mytilus edulis) and oysters (Ostrea edulis)[J]. Int J Food Microbiol, 2007, 113 (3): 296-302.

Richards G P. Shellfish-Associated Viral Disease Outbreaks[M]. Food Microbiology and Food Safety, 2006: 223-238.

Costantini V, Loisy F, Lynn J, et al. Human and Animal Enteric Caliciviruses in Oysters from Different Coastal Regions of the United States [J]. Appl Environ Microbiol, 2006, 72(3): 1800-1809.

Provost K, Dancho B A, Ozbay G, et al. Hemocytes are sites of enteric virus persistence within oysters[J]. Appl Environ Microbiol, 2011, 77(23):8360-8369.

Burkhardt W, Calci K R I. Selective accumulation may account for shellfish-associated viral illness[J]. Appl Environ Microbiol, 2000, 66: 1375-1378.

Le G F, Neill F H, Estes M K, et al. Detection and analysis of a small roundstructured virus strain in oyster simplicated in an outbreak of acute gastroenteritis[J]. Appl Environ Microbiol, 1996b, 62: 4268-4272.

GB 2744-1996, 海水贝类卫生标准[S].

Logan C, O'leary J J, Sullivan O O. Real-time reverse transcription-PCR for detection of rotavirus and adenovirus as causative agents of acute viral gastroenteritis in children[J]. J Clin Microbiol, 2006, 44: 3189-3195.

Sedmak G, Bina D, Macdonald J. Assessment of an enterovirus sewage surveillance system by comparison of clinical isolates with sewage isolates from Milwaukee, Wisconsin, collected August 1994 to December 2002[J]. Appl Environ Microbiol, 2003,69: 7181-7187.

Albinana G N, Miagostovich M P, Calqua B, et al. Analysis of adenoviruses and polyomaviruses quantified by qPCR as indicators of water quality in source and drinking-water treatment plants[J]. Water Res, 2009, 43: 2011-2019.

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