黄海春季和秋季中华哲水蚤脂类含量及组成分析

王延清; 李超伦; 刘梦坛; 金鑫; 王晓东; 孙永坤

黄海春季和秋季中华哲水蚤脂类含量及组成分析

1.
中国科学院海洋研究所海洋生态与环境重点实验室,山东青岛 266071;中国科学院大学,北京 100049
2.
中国科学院海洋研究所海洋生态与环境重点实验室,山东青岛 266071
3.
中国科学院胶州湾海洋生态系统国家野外科学观测研究站,山东青岛 266071

基金项目: 国家自然科学面上基金(41076099；41121064)；国家重点基础研究发展规划(973)项目(2011CB403604)。

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出版历程
- 收稿日期: 2012-10-27

Lipid content and composition of Calanus sinicus in the Yellow Sea in spring and autumn

1.
Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;University of Chinese Academy of Sciences, Beijing 100049, China
2.
Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
3.
Jiaozhou Bay Ecosystem Research Station,Chinese Academy of Sciences, Qingdao 266071, China

摘要

摘要: 脂类物质在哲水蚤的休眠、繁殖以及个体发育过程中发挥着重要作用，通过分析2010年11月(秋季)和2011年4月(春季)黄海中华哲水蚤Calanus sinicus的脂类组成，对脂类各组分的生理功能进行了初步探讨。结果表明，春季、秋季中华哲水蚤雌体脂类平均含量占干质量的10.6%和12%，C5期个体脂类含量偏高，占到干质量的17%。中华哲水蚤所含脂类主要由蜡脂和磷脂组成，两种脂类约占总脂的90%，三酰甘油含量约占总脂的6%。结合种群结构对脂类的生理作用进行了初步探讨，发现：(1)蜡脂作为主要的能量储存方式，其含量变化反映出不同发育期、不同季节中华哲水蚤的能量需求变化;(2)磷脂作为结构性脂类在中华哲水蚤个体生长与生殖过程中具有重要作用;(3)三脂酰甘油在中华哲水蚤中的含量不高，但是可能在脂类转化利用过程中发挥作用。
- 中华哲水蚤 /
- 黄海 /
- 脂类 /
- 蜡脂 /
- 磷脂 /
- 三脂酰甘油
Abstract: Lipid plays an important role in reproduction, food scarcity and diapause of zooplankton. The present research studied the role of lipids in Calanus sinicus, and samples were collected from the Southern Yellow Sea in autumn (November, 2010) and spring (April, 2011). The results show that the total lipid content of female C. sinicus was about 10.6%-12% of dry body mass, while C5 stage in spring had a higher percentage, about 17% of dry mass. The major components in lipid classes were wax ester (WE) and phospholipid (PL), and they together contributed about 90% to total lipid, while triglyceride (TAG) accounted for only 6%. The role of different lipid classes was discussed as follows: (1) Wax ester served as an important energy deposit. The fluctuated WE content of C. sinicus suggested the different strategies in energy needs and utilization of different development stages and seasons. (2) The coincidence of active reproduction and high content of PL in female indicated that PL plays an important role in the egg production of C. sinicus. (3) The composition of TAG was relatively lower than that of WE and PL in C. sinicus, but it might work in the transformation of different lipid classes.
- Calanus sinicus /
- southern Yellow Sea /
- lipids /
- wax ester /
- phospholipid /
- triglyceride

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参考文献(38)

Hagen W, Auel H. Seasonal adaptations and the role of lipids in oceanic zooplankton[J]. Zoology, 2001, 104(3/4): 313-326.

Michael T A, Michael T B, Martin J K. Lipids in aquatic ecosystem[M]// Kattner G, Hagen W. Lipids in marine copepods: latitudinal characteristics and perspective to global warming. New York: Springer, 2009: 257-280.

Kattner G, Hagen W, Lee R F, et al. Perspectives on marine zooplankton lipids[J]. Canadian Journal of Fisheries and Aquatic Sciences, 2007, 64(11): 1628-1639.

Lee R F, Hagen W, Kattner G. Lipid storage in marine zooplankton[J]. Marine Ecology Progress Series, 2006, 307: 273-306.

Falk-Petersen S, Hagen W, Kattner G, et al. Lipids, trophic relationships, and biodiversity in Arctic and Antarctic krill[J]. Canadian Journal of Fisheries and Aquatic Sciences, 2000, 57(1): 178-191.

Chen M R, Liu H B, Chen B Z. Effects of dietary essential fatty acids on reproduction rates of a subtropical calanoid copepod, Acartia erythraea[J]. Marine Ecology Progress Series, 2012, 455: 95-110.

Koski M, Yebra L, Dutz J, et al. The effect of egg versus seston quality on hatching success, naupliar metabolism and survival of Calanus finmarchicus in mesocosms dominated by Phaeocystis and diatoms[J]. Marine Biology, 2012, 159(3): 1-18.

Pond D, Harris R, Head R, et al. Environmental and nutritional factors determining seasonal variability in the fecundity and egg viability of Calanus helgolandicus in coastal waters off Plymouth, UK[J]. Marine Ecology Progress Series, 1996, 143(1): 45-63.

Campbell R, Dower J. Role of lipids in the maintenance of neutral buoyancy by zooplankton[J]. Marine Ecology Progress Series, 2003, 263: 93-99.

Clark K A J, Brierley A S, Pond D W. Composition of wax esters is linked to diapause behavior of Calanus finmarchicus in a sea loch environment[J]. Limnology and Oceanography, 2012, 57(1): 65-75.

Pond D W. The physical properties of lipids and their role in controlling the distribution of zooplankton in the oceans[J]. Journal of Plankton Research, 2012, 34(6): 443-453.

Pond D W, Tarling G A. Phase transitions of wax esters adjust buoyancy in diapausing Calanoides acutus[J]. Limnology and Oceanography, 2011, 56(4): 1310-1318.

Falk-Petersen S, Mayzaud P, Kattner G, et al. Lipids and life strategy of Arctic Calanus[J]. Marine Biology Research, 2009, 5(1): 18-39.

Lee R F, Hirota J, Barnett A M. Distribution and importance of wax esters in marine copepods and other zooplankton[J]. Deep-Sea Research, 1971, 18(12): 1147-1165.

Miller C B, Crain J A, Morgan C A. Oil storage variability in Calanus finmarchicus[J]. ICES Journal of Marine Science: Journal du Conseil, 2000, 57(6): 1786-1799.

Thorisson K. How are the vertical migrations of copepods controlled？ [J].Journal of Experimental Marine Biology and Ecology, 2006, 329(1): 86-100.

陈清潮. 中华哲水蚤的繁殖、性比率和个体大小的研究[J]. 海洋与湖沼,1964, 6(3): 272-288.

孙松, 王世伟, 李超伦. 黄海中华哲水蚤 C5 发育期油脂积累初步研究[J].海洋与湖沼, 2011, 42(2):165-169.

Liu M T, Li C L, Sun S. Seasonal variation in fatty acid composition of seston and the copepod Calanus sinicus (Brodsky, 1962) in Jiaozhou Bay and its trophic implications[J]. Oceanology and Limnology Sinica, 2011, 29(6): 1164-1173.

刘梦坛, 李超伦, 孙松. 脂肪酸对中华哲水蚤摄食两种海洋微藻的指示作用[J]. 生态学报, 2011, 31(4): 933-942.

Ohman M D. Freezing and storage of copepod samples for the analysis of lipids[J]. Marine Ecology Progress Series, 1996, 130: 295-298.

Folch J, Lees M, Sloane-Stanley G. A simple method for the isolation and purification of total lipids from animal tissues[J]. Journal of Biological Chemistry, 1957, 226(1): 497-509.

Hopkins C, Seiring J, Nyholmen O. Ecological energetics from total lipid and total protein: fact and artifact using a gravimetric method for lipid and a biuret method for protein[J]. Oceanography and Marine Biology, 1984, 22: 211-261.

Ohman M D. On the determination of zooplankton lipid content and the occurrence of gelatinous copepods[J]. Journal of Plankton Research, 1997, 19(9): 1235-1250.

翁学传, 王从敏. 黄海冷水团边界及温盐范围的确定 // 中国湖沼学会水文气象学术会议(1980)论文集. 北京:科学出版社, 1982:61-70.

于非, 张志欣, 刁新源, 等. 黄海冷水团演变过程及其与邻近水团关系的分析[J].海洋学报, 2006, 28(5): 26-34.

Sun S. Over-summering strategy of Calanus sinicus . GLOBEC IntNewsl, 2005, 11:34. http://www.igbp-cnc.org.cn/UserFiles/IGBP-Sun%20Song.pdf

Wang R, Zuo T. The Yellow Sea Warm Current and the Yellow Sea Cold Bottom Water, their impact on the distribution of zooplankton in the southern Yellow Sea[J]. Journal korean society of oceamography, 2004, 39(1): 1-13.

Zhang G T, Sun S, Yang B. Summer reproduction of the planktonic copepod Calanus sinicus in the Yellow Sea: influences of high surface temperature and cold bottom water[J]. Journal of Plankton Research, 2007, 29(2): 179-186.

Pu X M, Sun S, Yang B, et al. Life history strategies of Calanus sinicus in the southern Yellow Sea in summer[J]. Journal of Plankton Research, 2004, 26(9): 1059-1068.

Wang S W, Li C L, Sun S, et al. Spring and autu慭牮挠桲?偰慲灯敤牵獣???の?は?????????????ㄠ???????戼爯?孭?㈠嵩??整敨?删?????慷爠湓敥獡???吮???楲灩楮摥猠?楣湯?瑯桧敹?浐敲獯潧灲敥汳慳朠楓捥?捩潥灳攬瀠漲搰???攠洳??愺甠猱猲椳愭?瀳爳椮渼换敲瀾獛???攠浈??坡慮硳?敮猠瑊攠牌?甠瑔楨汥椠穬慯瑮楧漠湡?摤甠牳楨湯杲?猠瑴慥牲癭愠瑦楥潥湤孩?嵧???潮浤灩慴物慯瑮椠癩敮??楩潥捬桤攭浣楡獵瑧牨祴?愼湥摭￣偃桡祬獡楮潵汳漠杰祡?偩慦物瑣?????潭派瀬愠牡慳琠楤癥整??業潩据桥敤洠楦獲瑯牭礠?????????㈠??????????????扭牮?孬??嵹?卡慮牤朠敏湣瑥?????慡瑰瑨敹測?刱???漬爠渲改爨????攷琹?愭永??伮渼?瑲栾敛″渳畝琠牌楥瑥椠潒渠?愬渠摈?浲敯瑴慡戠潊氮椠獗浡?漠晥?穴潥潲灳氠慩湮欠瑴潲湯??塣????楯灯楰摬獡?楫湴??攠浡??愠汮慥湫畴獯?栠敡汮杤漠汴慨湥搠楧捥畯獧??数浨??条牬愠穤楩湳杴??敢浵?剩楯摮搠畯汦瀠桷楡慸?獥楳湴敥湲獳椠獩??敭浡?孩?嵥???潰略牰湯慤汳?潊晝?琠桌敩??慯牬楯湧敹??楮潤氠潏杣楥捡慮汯??獡獰潨捹椬愠琱椹漷渳?漠昱?琨栲攩?唠渲椲琷攭搲″?椮渼杢摲漾浛???????????㈠???????????erner I. Respiration and lipid content of the Arctic copepod Calanus hyperboreus overwintering 1 m above the seafloor at 2 300 m water depth in the Fram Strait[J]. Marine Biology, 2003, 143(2): 275-282.

Scott C L, Kwasniewski S, Falk-Petersen S, et al. Lipids and life strategies of Calanus finmarchicus, Calanus glacialis and Calanus hyperboreusin late autumn, Kongsfjorden, Svalbard[J]. Polar Biology, 2000, 23(7): 510-516.

Diel S, Tande K. Does the spawning of Calanus finmarchicus in high latitudes follow a reproducible pattern？ [J].Marine Biology, 1992, 113(1): 21-31.

Kattner G, Graeve M, Hagen W. Ontogenetic and seasonal changes in lipid and fatty acid alcohol compositions of the dominant Antarctic copepods Calanus propinquus, Calanoides acutus and Rhincalanus gigas[J]. Marine Biology, 1994, 118(4): 637-644.

Tande K, Henderson R. Lipid composition of copepodite stages and adult females of Calanus glacialis in Arctic waters of the Barents Sea[J]. Polar Biology, 1988, 8(5): 333-339.

王世伟. 黄海中华哲水蚤繁殖、种群补充与生活史研究 . 青岛:中国科学院海洋研究所, 2009.

Lee R F, Nevenzel J C, Lewis A. Lipid changes during life cycle of marine copepod, Euchaeta japonica marukawa[J]. Lipids, 1974, 9(11): 891-898.

Saito H, Tsuda A. Egg production and early development of the subarctic copepods Neocalanus cristatus, N. plumchrus and N. flemingeri[J]. Deep-Sea Research Part I: Oceanographic Rese

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