渤海湾肠道病毒的季节分布及其污染类型分析
Analysis on the seasonal distribution and pollution type of enterovirus in Bohai Bay
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摘要: 肠道病毒是一类水传播胃肠道疾病的重要病原体。为了解感染性肠道病毒在海水环境中的季节分布规律、污染类型以及与流行病学的关系,于2010年12月到2011年10月应用能够提供病毒感染性信息的细胞培养结合实时定量PCR(ICC-qPCR)方法,分4个季节对渤海湾天津近岸海域表层海水中肠道病毒进行了监测。500 mL海水经超滤浓缩,48 h细胞培养,然后用qPCR检测得到四季海水样品中感染性肠道病毒的浓度为0.2~196 PFU/L,平均值为60 PFU/L,其中夏季和秋季肠道病毒浓度较高,其平均值分别为82 PFU/L和110 PFU/L。秋季阳性检出率最高(85.7%),其次是冬季(71.4%)。经测序分析,其主要污染类型是脊髓灰质炎 Ⅰ 型疫苗株,此外,还检测出危及公众健康的一株类口服脊灰 Ⅰ 型疫苗脊灰和一株脊灰疫苗衍生株。由此可见,肠道病毒的季节分布和主要污染类型与临床上的流行趋势基本一致,海洋作为一个天然的受纳水体为病毒提供了良好的栖息场所和传播机会,对公众健康存在着潜在的威胁。因此,为减少相关疾病的暴发,应重点加强肠道病毒流行季节的海洋水质监测。
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关键词:
- 感染性肠道病毒 /
- 细胞培养结合实时定量PCR /
- 渤海湾 /
- 表层海水
Abstract: Enteroviruses are one of the main agents of water-borne gastroenteritis. In order to learn the relationship between seasonal distribution as well as pollution type of infectious enterovirus and epidemiology,the integration of cell culture techniques and quantitative PCR (ICC-qPCR) was employed,surface seawater samples were seasonally collected from November 2010 to October 2011 to identify the contamination by infectious enteroviruses in winter seawater samples of Bohai Bay,Tianjin,China. 500 mL seawater was concentrated,cultivated for 48h,and then quantified by qPCR. The concentration of infectious enteroviruses were estimated at 0.2~196 PFU/L,average value was 60 PFU/L. During the four seasons,the concentration of enteroviruses in Summer and Autumn were 82 PFU/L and 110 PFU/L,respectively,which were much higher than the other two seasons. While the positive rate in Autumn is highest (85.7%),followed by Winter (71.4%). The main contaminated type was characterized as poliovirusⅠvaccine strains by sequencing. Besides,there were two strains of OPV-like Poliovirus TypeⅠand vaccine-derived poliovirus (VDPV). These results presented that the seasonal distribution and the main pollution types of enteroviruses were almost the same as epidemiology. Therefore,the ocean as the natural container of sewage,which provides a good opportunity for virus to survive and spread. There was a potential threat to public health. In order to avoid disease breaking out,the monitoring of enteroviruses in marine during epidemic season must be strengthened. -
Da Silva A K, Le Saux J C, Parnaudeau S, et al. Evaluation of removal of noroviruses during wastewater treatment, using real-time reverse transcription-PCR: different behaviors of genogroups I and Ⅱ[J]. Appl Environ Microbiol, 2007, 73: 7891-7897. Haramoto E, Katayama H, Utagawa E, et al. Development of sample storage methods for detecting enteric viruses in environmental water[J]. J Virol Methods, 2008, 151: 1-6. Lodder W J, De Roda H A M. Presence of noroviruses and other enteric viruses in sewage and surface waters in The Netherlands[J]. Appl Environ Microbiol, 2005, 71: 1453-1461. Mcquaig S M, Scott T M, Harwood V J, et al. Quantification of human polyomaviruses JC virus and BK virus by Taqman Quantitative PCR and comparison to other water quality indicators in water and fecal samples[J]. Appl Environ Microbiol, 2009, 75: 3379-3388. Kocwa-Haluch R. Waterborne enteroviruses as a hazard for human health[J]. Pol J Environ Stud, 2001, 10:485-487. Wuertze S, Wang D, Reischer G H, et al. Library-Independent Bacterial Source Tracking Methods[M]// Microbial Source Tracking: Methods, Applications and Case Studies. New York, USA: Springer Science + Business Media, 2011: 61-112. Mattijnssens J, Ciarlet M, Heiman E, et al. Full genome-based classification of rotaviruses reveals a common origin between human Wa-Like and porcine rotavirus strains and human DS-1-like and bovine rotavirus strains[J]. J Virol, 2008, 82 (7) : 3204-3219. Li D, Gu A Z, He M, et al. UV inactivation and resistance of rotavirus evaluated by integrated cell culture and real-time RT-PCR assay[J]. Water Res, 2009, 43 (13) : 3261-3269. Li D, Gu A Z, Yang W. An integrated cell culture and reverse transcription quantitative PCR assay for detection of infectious rotaviruses in environmental waters[J]. J Microbiol Meth, 2010, 82: 59-63. 明红霞, 董玉波, 任莹利, 等. ICC-qPCR快速定量渤海湾表层海水中感染性轮状病毒[J].海洋通报, 2012, 31 (3) : 53-58. Zhang M L, Zhao H B, Yang J, et al. Detection and quantification of enteroviruses in coastal seawaters from Bohai Bay, Tianjin, China[J]. J Environ Sci China, 2010, 22 (1) :150-155. Ming H X, Zhu L, Zhang Y. Rapid quantification of infectious enterovirus from surface water in Bohai Bay, China using an integrated cell culture-qPCR assay[J]. Mar Pollut Bull, 2011, 62 (10) : 2047-2054. 张崇淼.水环境中肠道病原体的PCR检测方法与健康风险评价研究. 西安:西安建筑科技大学, 2008. Hot D, Legeay O, Jacques J, et al. Detection of somatic phages, infectious enteroviruses and enterovirus genomes as indicators of human enteric viral pollution in surface water[J]. Water Res, 2003, 37: 4703-4710. Loh P C, Fujioka R S, Lau L S. Recovery, survival and dissemination of human enteric viruses in ocean water receiving sewage in Hawail[J]. Water Air Soil Poll, 1979, 12 (2) : 197-217. 郑渡平, 张礼壁, 侯晓辉, 等. 1989-1994年中国脊髓灰质炎病毒的分子流行病学[J]. 病毒学报, 1995, 11 (4) :289-297. 柯昌文, 吴承民, 李 杰, 等. 广东省1985-1993 年脊髓灰质炎流行野毒株的基因分析[J]. 中国计划免疫, 1997, 3 (6) : 268-272. 欧阳雅博.天津市儿童病毒性腹泻病原体与污水中肠道内病毒的关系. 天津:中国人民解放军军事医学科学院卫生学环境医学研究所, 2010. 张岩, 季圣翔, 张晓丽, 等. 2010年山东省环境污水中脊髓灰质炎病毒的监测及其基因特征分析[J]. 病毒学报, 2011, 27 (4) : 327-341. Shen H, Bai B, Hou J, et al. OPV-like poliovirus type 1 detection in patients with severe acute respiratory syndrome[M]. Infection, 2012. WHO. Performance of acute flaccid paralysis (AFP) surveillance and incidence of poliomyelitis, 2006[M]. WER, 2006, 81 (46) : 440-444. CDC. Outbreak of poliovirus in adults-Namibia, 2006[M]. MMWR, 2006, 55 (44) : 1198-1201. Blomqvist S, Savolainen C, Laine P, et al. Characterization of a highly evolved vaccine-derived poliovirus type 3 isolated from sewage in Estonia[J]. J Virol, 2004, 78: 4876-4883. 吴照, 温宁, 梁晓峰. 环境监测在消灭脊髓灰质炎过程的作用[J]. 中国疫苗和免疫, 2011, 17 (2) : 173-176.
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