海洋石油降解菌剂在大连溢油污染岸滩修复中的应用研究

郑立; 崔志松; 高伟; 韩彬; 杨佰娟; 王绍良; 李倩; 周文俊; 张魁英; 曹磊

海洋石油降解菌剂在大连溢油污染岸滩修复中的应用研究

国家海洋局第一海洋研究所海洋生态研究中心, 山东青岛 266061

基金项目: 海洋公益性行业专项项目(200705011;200805039);国家自然科学基金(41076108,40906062);国家海洋局第一海洋研究所基本科研业务费专项资金(2010G23);国家海洋局近岸海域生态环境重点实验室资助项目(201008)。

计量
- 文章访问数: 1714
- HTML全文浏览量: 32
- PDF下载量: 1962
- 被引次数: 0
出版历程
- 收稿日期: 2011-04-19
- 修回日期: 2011-09-28

Field study of the bioremediation of oil-contaminated Dalian beach by marine oil-degrading bacterial agent

First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China

摘要

摘要: 对海洋石油降解菌群DC10的降解特性进行了研究,研制了基于该菌群的降解菌剂,并用于大连实际溢油岸滩生物修复实验,考察了降解菌剂对潮间带和潮上带油污的降解作用,通过分析C17/藿烷、C18/藿烷及总烷烃和总芳烃的降解率来评价其降解效果。降解菌群DC10在实验室条件下对石油的降解率高于各组成菌株,一周时间的石油降解率比对照提高了60%左右,能降解大部分的烷烃和芳烃。以DC10冻干菌粉辅以营养盐溶液研制降解菌剂,该菌剂在大连岸滩油污生物修复试验中显示出显著的降解效果。在为期12 d的潮间带油污生物修复试验中,喷洒菌剂处理的C17/藿烷和C18/藿烷降解率相对于自然风化处理分别提高了40%和30%,而总烷烃和总芳烃降解率分别提高了80%和72%。在为期85 d的潮上带油污生物修复试验中,从C17/藿烷和C18/藿烷的降解率来看,喷洒菌剂处理对油污的降解程度仅略高于自然风化,但总烷烃和总芳烃的降解率分别提高了近30%和20%。
- 生物修复 /
- 海洋溢油 /
- 石油烃降解菌
Abstract: A marine oil-degrading bacterial consortium DC10 was prepared to an oil-degrading agent and further applied to the bioremediation of crude oil-contaminated Dalian beach. Field studies were carried out at the intertidal zone and supratidal zone respectively, and the effects of the oil-degrading agent were investigated by evaluating the decline of C17/hopane, C18/hopane, total petroleum alkanes and total petroleum aromatics.The oil-degrading consortium DC10 exhibited much higher degradation rates than the single degrader comprising consortium DC10 under laboratory conditions. Compared to the sterile control, the degradation rates of consortium DC10 increased by 60% in 7 days, and most alkanes and aromatics in oil were disappeared. The oil-degrading agent supplemented with essential nutrient salts was proved to be effective in the field study on the bioremediation of oil-contaminated Dalian beach. At the intertidal zone plot, the C17/hopane and C18/hopane degradation rates of bioremediation treatment increased by approximately 40% and 30% in 12 d, respectively, compared to the natural weathering. While the total alkanes and total aromatics degradation rates of bioremediation treatment increased by approximately 80% and 72% in 12 d. In the case of supratidal zone plot, the C17/hopane and C18/hopane degradation rates of bioremediation treatment was only slightly higher than natural weathering in 85 d. While the total alkanes and total aromatics degradation rates of bioremediation treatment increased by approximately 30% and 20% in 85 d, respectively.
- bioremediation /
- marine oil spill /
- petroleum-degrading bacteria

HTML全文

参考文献(25)

YEMASHOVA N A, MURYGINA V P, ZHUKOV D V, et al.Biodeterioration of crude oil and oil derived products: a review[J]. Rev Environ Sci Biotechnol, 2007, 6(4): 315-337.

REIA Hosokawa, MOTONORI Nagai, MASAAKI Morikawa, et al. Autochthonous bioaugmentation and its possible application to oil spills[J].WORLD JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 2009, 25(9): 1519-1528.

XIA W X, ZHENG X L, LI J C, et al.Degradation of crude oil by indigenous microorganisms supplemented with nutrients[J]. J Envir Sci, 2005, 17(4), 659-661.

LI H, ZHAO Q, BOUFADEL M C, VENOSA A D, A universal nutrient application strategy for the bioremediation of oil-polluted beaches. Marine Pollut Bull 2007, 54(8):1146-1161.

RON E, ROSENBERG E. Role of Fertilizers: Biostimulation. Handbook of hydrocarbon and lipid microbiology, 2010, Part 24, 2521-2529, DOI: 10.1007/978-3-540-77587-4-184.

BRAGG J R, PRINCE R C, HARNER E J, et al.Effectiveness of bioremediation for the Exxon Valdez oil spill[J]. Nature, 1994, 368: 413-418.

NIKOLOPOULOU M, KALOGERAKIS N.Biostimulation Strategies for Enhanced Bioremediation of Marine Oil Spills Including Chronic Pollution. Handbook of hydrocarbon and lipid microbiology, 2010, Part 24, 2501-2504, DOI: 10.1007/978-3-540-77587-4_187

TSUTSUMI H, KONO M, TAKAI K, et al.Bioremediation on the shore after an oil spill from the Nakhodka in the Sea of Japan: Ⅲ.Field test of a bioremediation agent with microbiological cultures for the treatment of an oil spill[J]. Mar Pollu Bull, 2000, 40(4), 320-324.

XIA W X, LI J C, ZHENG X L, et al.Enhanced biodegradation of diesel oil in seawater supplemented with nutrients[J]. Engin Life Sci, 2006, 6(1): 80-85.

HII Y S, LAW A T, SHAZILL N A M, et al. Biodegradation of Tapis blended crude oil in marine sediment by a consortium of symbiotic bacteria[J]. 2009, 63(2):142-150.

韩平, 郑立, 崔志松, 等. 胜利油田滩涂区石油降解菌的筛选、鉴定及其多样性分析[J]. 应用生态学报, 2009, 20(5): 1202-1208.

崔志松, 郑立, 杨佰娟, 等,. 两种海洋专性解烃菌降解石油的协同效应[J]. 微生物学报, 2010, 50(3): 350-359.

OH Y S, SIM D S, KIM S J. Effects of nutrients on crude oil biodegradation in the upper intertidal zone[J]. Mar Pollut Bull, 2001, 42(12), 1367-1372.

PRINCE R C, ELMENDORF D L, LUTE J R, et al.17α (H),21β (H)-Hopane as a conserved internal marker for estimating the biodegradation of crude oil[J]. Environ Sci Technol, 1994, 28(1): 142-145.

OH Y S, CHOI W Y, LEE Y H, et al.Biological treatment of oil-contaminated sand: comparison of oil degradation based on thin-layer chromatography/flame ionization detector and respirometric analysis[J]. Biotech Let, 2000, 22(7): 595-598.

MARIA N, NICOLAS K. Biostimulation strategies for fresh and chronically pollutedmarine environments with petroleum hydrocarbons[J]. Journal of Chemical Technology and Biotechnology, 2009, 84(6): 802-807.

NIKOLOPOULOUA M, KALOGERAKIS, N. Enhanced bioremediation of crude oil utilizing lipophilic fertilizers combined with biosurfactants and molasses[J]. Mar Pollut Bull, 2008, 56, 1855-1861.

LEE K, TREMBLAY GH, COBANLI SE.Bioremediation of oiled beach sediments: Assessment of inorganic and organic fertilizers //Proceedings of 1995 Oil Spill Conference.Washington, DC: American Petroleum Institute, 1995:107-113.

KNEZEVICH V O, KOREN E, RON Z, et al. Petroleum bioremediation in seawater using guano as the fertilizer[J]. Bioremediation J, 2006, 10:83-91.

DELAUNE R, LAN D, WRIGHT L. Projected Impact of Deepwater HorizonOil Spill on U.S. Gulf Coast Wetlands[J]. Soil Sci Soc Am J, 2011, 75(5):1-11.

SWANNELL R P J, LEE K, MCDONAGH M. Field evaluations of marine oil spill bioremediation[J]. Microbiological Reviews, 1996, 60(2), 342-365.

ZHU X, VENOSA A D, SUIDAN M T. Literature review on the use of commercial bioremediation agents for cleanup of oil-contaminated estuarine environments.EPA.2004, EPA/600/R-04/075.

胥九兵, 迟建国, 邱维忠, 等, 石油降解菌剂的研制及其在石油污染土壤修复中的应用[J]. 生物加工过程, 2009, 7(6): 21-24.

黄廷林, 徐金兰, 唐智新, 等,生物菌剂对石油污染土壤生物修复作用的研究[J]. 环境科学, 2009, 30(6): 838-1843.

YOUNG-SOOK O, DOO-SEUP S, SANG-JIN K. Effects of nutrients on crude oil biodegeadation in the upper intertidal zone[J], Marine Pollution Bulletin, 2001, 42(12), 1367-1372.

施引文献

资源附件(0)

访问统计