东海原甲藻核酸和蛋白对环境中氮磷浓度变化的响应

刘云; 宋秀贤; 曹西华; 袁涌铨; 俞志明

东海原甲藻核酸和蛋白对环境中氮磷浓度变化的响应

1.
中国科学院海洋研究所海洋生态与环境科学重点实验室, 山东青岛 266071;中国科学院研究生院, 北京 100049
2.
中国科学院海洋研究所海洋生态与环境科学重点实验室, 山东青岛 266071

基金项目: 国家自然科学基金(30970522);国家重点基础研究发展计划("九七三"项目,2010CB428706);国家"八六三"计划专题项目(2008AA09Z107)。

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出版历程
- 收稿日期: 2011-06-01
- 修回日期: 2011-09-26

Responses of nucleic acid and protein content in Prorocentrum donghaiense Lu to different nitrogen and phosphorus concentrations

1.
Key Laboratory of Marine Ecology and Environmental Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Graduate School, Chinese Academy of Sciences, Beijing 100049, China
2.
Key Laboratory of Marine Ecology and Environmental Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China

摘要

摘要: 通过设定不同的氮(N)、磷(P)浓度一次性培养东海原甲藻,研究了该藻的生长状况及不同生长阶段单位细胞DNA,RNA及蛋白质含量的变化,结果表明,不同氮磷浓度对东海原甲藻的生长产生显著影响,最终生物量与初始的氮磷浓度呈正相关,不同培养组的最大生长率存在显著差异(P<0.05);DNA含量与培养液中氮浓度相关性极显著(P<0.01),但与磷浓度相关性不显著(P>0.05);RNA含量与氮磷浓度均呈极显著相关(P<0.01),而且在不同的生长阶段其含量也会发生变化,随着培养时间的延长,各培养组的RNA含量显著下降(P<0.05);RNA/DNA比值与氮磷浓度均显著相关(P<0.05),而且与生长率呈线性相关关系。培养液中氮浓度显著影响到东海原甲藻单位细胞总蛋白含量,磷缺乏也会使总蛋白含量显著降低。
- 东海原甲藻 /
- DNA /
- RNA /
- RNA/DNA比值 /
- 蛋白质 /
- 生长率
Abstract: Changes in growth, cellular DNA, RNA and total protein content related to nitrogen(N) and phosphorus(P) concentrations were studied in a marine dinoflagellate Prorocentrum donghaiense Lugrown in batch cultures. The results show that the growth of P. donghaiense is significantly affected by N and P concentrations. The ultimate biomass depended on the initial N and P concentrations. The maximum growth varied distinctly under different N or P treatments(P<0.05). DNA content had significant correlation with N concentrations in the cultures but not with P. RNA content was strongly associated with both N and P concentrations(P<0.01). Different growth stages could also change cellular RNA content. The RNA content decreased along with the extension of experimental time.RNA/DNA ratio had significant correlation with the N and P concentrations. There appeared to be a linear relationship between the growth rate and the ratio of RNA/DNA. Cellular total protein content was significantly affected by N concentrations. P deficiency could also reduce cellular protein content.
- Prorocentrum donghaiense Lu /
- DNA /
- RNA /
- RNA/DNA /
- protein /
- growth rate

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