应用新一代测序技术测定大室别藻苔虫线粒体基因组全序列

申欣; 田美; 朱长保; 刘会莲; 赵方庆

应用新一代测序技术测定大室别藻苔虫线粒体基因组全序列

1.
淮海工学院海洋学院, 江苏连云港 222005;中国科学院北京生命科学研究院, 北京 100101
2.
淮海工学院海洋学院, 江苏连云港 222005
3.
中国科学院海洋研究所, 山东青岛 266071
4.
中国科学院北京生命科学研究院, 北京 100101

基金项目: 国家自然科学基金(40906067);中国科学院基金(Y064021BJ1;0869011BJ5);江苏省"青蓝工程"人才基金(苏教师[2010]27号)。

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出版历程
- 收稿日期: 2011-06-26
- 修回日期: 2011-09-26

Determination of Membranipora grandicella complete mitochondrial genome sequence based on next-generation sequencing technology

1.
College of Marine Science, Huaihai Institute of Technology, Lianyungang 222005, China;Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing 100101, China
2.
College of Marine Science, Huaihai Institute of Technology, Lianyungang 222005, China
3.
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
4.
Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing 100101, China

摘要

摘要: 线粒体基因组的获得传统上通常是采取长PCR结合鸟枪法或步移法测序。近年来新一代测序技术蓬勃发展,以454,Solexa和SOLiD测序平台为代表。应用新一代高通量测序技术(Solexa)并结合巢式PCR扩增,完成了大室别藻苔虫(Membranipora grandicella)的线粒体基因组全序列。大室别藻苔虫线粒体基因组是目前国际上获得的第一条苔藓动物软壁亚目线粒体基因组全序列,基因组全长为15861 bp,比已完成的4种苔藓动物线粒体基因组略大。大室别藻苔虫线粒体基因组包含13个蛋白质编码基因、2个核糖体RNA基因和20个转运RNA基因。通过与已测得的苔藓动物进行比较,发现目前已完成的5个苔藓动物线粒体基因组的基因排列顺序显著不同。苔藓动物线粒体基因组基因排列的比较,今后可能会成为探讨该类群系统演化关系的重要信息来源。
- 大室别藻苔虫 /
- 线粒体基因组 /
- 新一代测序技术 /
- 巢式PCR /
- 基因排列
Abstract: Traditionally, the mitochondrial genome sequences were usually obtained by shotgun or primers-walking combination with long PCR. The next-generation sequencing technologies are developing fastly in recent years, such as 454, Solexa and SOLiD sequencing platform. Membranipora grandicella complete mitochondrial genome sequence was determined based on next-generation high-throughput sequencing technology (Solexa) and nested PCR amplification. The complete mitochondrial genome of M. grandicella is 15,861 bp in length, which is the first representative from the suborder Malacostegina (Bryozoa), and is relatively larger when compared with mtDNAs of other study bryozoans. The mitochondrial genome contains 13 protein-coding genes, two ribosomal RNAs and 20 transfer RNAs. Compared with other study bryozoans, gene arrangements of five bryozoans mitochondrial genomes are significantly different. Comparison of bryozoans mitochondrial gene arrangements can be important information sources for exploring the phylogenetic relationships within the group.
- Membranipora grandicella /
- mitochondrial genome /
- next-generation sequencing /
- nested PCR /
- gene arrangement

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参考文献(12)

王曦,汪小我,王立坤,等.新一代高通量测序数据的处理与分析[J].生物化学与生物物理进展,2010,37(8):834-846.

HELFENBEIN K G,FOURCADE H M,VANJANI R G,et al. The mitochondrial genome of Paraspadella gotoi is highly reduced and reveals that chaetognaths are a sister group to protostomes[J]. Proc Natl Acad Sci USA,2004,101(29):10639-10643.

DELLAPORTA S L,XU A,SAGASSER S,et al. Mitochondrial genome of Trichoplax adhaerens supports placozoa asthe basal lower metazoan phylum[J]. Proc Natl Acad Sci USA,2006,103(23):8751-8756.

SHEN X,MA XY,REN JF,et al. A close phylogeneticrelationship between Sipuncula and Annelida evidenced from the complete mitochondrial genome sequence of Phascolosoma esculenta[J]. BMC Genomics,2009(10):136-146.

申欣,吴志刚.单环刺螠线粒体基因组全序列的获得——长PCR结合鸟枪法测序[J]. 海洋科学,2010,34(12):26-29.

WAESCHENBACH A M,TELFORD J,PORTER J S,et al. The complete mitochondrial genome of Flustrellidra hispida and the phylogenetic position of Bryozoa among the Metazoa[J]. Mol Phylogenet Evol,2006,40(1):195-207.

JANG KH,HWANG U W. Complete mitochondrial genome of Bugula neritina (Bryozoa,Gymnolaemata,Cheilostomata):phylogenetic position of Bryozoa and phylogeny of lophophorates within the Lophotrochozoa[J].BMC Genomics,2009,10:167.

SUN MA,WU ZG,SHEN X,et al. The complete mitochondrial genome of Watersipora subtorquata (Bryozoa,Gymnolaemata,Cheilostomida) with poylogentic consideration of Bryozoa[J]. Gene,2009,439(1-2):17-24.

SUN MA,SHEN X,LIU HL,et al. Complete mitochondrial genome of Tubulipora flabellaris (Bryozoa:Stenolaemata):the first representative from the class Stenolaemata with unique gene order[J]. Marine Genomics,2011,doi:10.1016/j.margen.2011. 03.006.

SIMPSON J T,WONG K,JACKMAN S D,et al. ABySS:a parallel assembler for short read sequence data[J].Genome Res. 2009,19(6):1117-1123.

WYMAN S K,JANSEN R K,BOORE J L.Automatic annotation of organellar genomes with DOGMA[J]. Bioinformatics,2004,20(17):3252-3255.

LOWE T M,EDDY S R. tRNAscan-SE:a program for improved detection of transfer RNA genes in genomic sequence[J]. Nucleic Acids Res,1997,25(5):955-964.

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