石斑鱼肠道原籍嗜冷杆菌(<em> Psychrobacter</em>SP.)SE6作为益生菌的体内外评价

杨红玲; 马如龙; 孙云章

石斑鱼肠道原籍嗜冷杆菌( PsychrobacterSP.)SE6作为益生菌的体内外评价

1.
集美大学水产学院福建省高校水产科学技术与食品安全重点实验室, 福建厦门361021
2.
集美大学水产学院福建省高校水产科学技术与食品安全重点实验室, 福建厦门361021;集美大学水产学院饲料检测与安全评价厦门市重点实验室, 福建厦门361021

基金项目: 国家自然科学基金(30600461);福建省高校杰出青年科研人才计划(JA10183);福建省高校水产科学技术与食品安全重点实验室基金(2009J102)。

计量
- 文章访问数: 1621
- HTML全文浏览量: 14
- PDF下载量: 43
- 被引次数: 0
出版历程
- 收稿日期: 2011-04-09
- 修回日期: 2011-08-09

Assessment of Psychrobacter sp. SE6 from the gut of grouper (Epinephelus coioides) as a novel probiont in vitro and in vivo

1.
Key Laboratory of Science and Technology for Aquaculture and Food Safety of Fujian Province University, Fisheries College, Jimei University, Xiamen 361021, China
2.
Key Laboratory of Science and Technology for Aquaculture and Food Safety of Fujian Province University, Fisheries College, Jimei University, Xiamen 361021, China;Key Laboratory of Xiamen City for Feed Detection and Safety Evaluation, Fisheries College, Jimei University, Xiamen 361021, China

摘要

摘要: 研究了石斑鱼肠道原籍嗜冷杆菌SE6在体外的抑菌特性及对人工胃肠液的耐受特性,在此基础上研究了饲料中添加SE6(10⁸CFU/g)对斜带石斑鱼生长性能和免疫功能的影响。体外试验结果表明:SE6对副溶血弧菌、哈维氏弧菌、梅氏弧菌和金黄色葡萄球菌等常见水产致病菌均有一定的抑制作用。pH为4.0的人工胃液能促进SE6的生长,处理2和4 h存活率分别为110.7%和173.0%;pH为3.0的人工胃液处理2 h后活菌数为1.59×10⁵ CFU/cm³,存活率为1.36%,但SE6不能耐受pH为2.0的人工胃液。经浓度分别为0.1%,0.3%,0.5%和0.7%的胆盐处理3 h后,SE6的存活率分别为89.17%,88.33%,77.17%和12.33%。动物试验结果显示:投喂SE6 60 d后试验组饵料系数(0.95±0.01)显著低于对照组(1.03±0.02)。试验组血清补体C3在试验全期均高于对照组,补体C4含量在投喂SE6 60 d时显著上升。血清IgM含量在投喂30 d时显著上升。总之,嗜冷杆菌SE6能抑制多种常见病原菌的生长,而且对模拟胃肠道环境具有一定的耐受能力,添加到饲料中能显著提高石斑鱼饵料利用率,增强动物的免疫功能。
- 嗜冷杆菌 /
- 斜带石斑鱼 /
- 抑菌活性 /
- 生长性能 /
- 免疫
Abstract: The antibacterial activity and tolerance to mimic gastrointestinal environments of the probiotic Psychrobacter sp. SE6 isolated from the gut of grouper(Epinephelus coioides)were evaluated in vitro. On the base of the in vitro results, a 60 d feeding trial was conducted to assess the effect of dietary administration(10⁸ CFU/g) of SE6 on the growth, feed utilization and immune responses of E.coioides.In vitro results show that SE6 exhibits antagonistic activity against pathogenic Vibrio parahaemolyticus, V.harveyi, V.metschnikovi and Staphyloccocus aureus.After 2 and 4 h of incubation with artificial gastric juice at pH 4.0, the survival ratio of SE6 is 110.7% and 173%, respectively. After treated with artificial gastric juice at pH 3.0 for 2 h, the survival ratio and the survival count are 1.36% and 1.59×10⁵CFU/cm³, respectively. However, SE6 cannot survive in artificial gastric juice at pH 2.0.Psychrobacter sp. SE6 exhibits good tolerance to artificial intestinal juice (different concentrations of bile salt and trypsin), the survival ratios of SE6 are 89.17%, 88.33%, 77.17% and 12.33% respectively after incubated in artificial intestinal juice with 0.1%, 0.3%, 0.5% and 0.7% bile salt for 3 h. In vivo results show that Psychrobacter sp. SE6 significantly decreases the feed conversion ratio (FCR) of E.coioides.Serum lysozyme activity shows no significant differences between the probiotic group and the control group during the whole experiment period, while an increasing trend of total superoxide dismutase (T-SOD) activity in the probiotic group is observed. Serum complement component 3 (C3) level of the probiotic group is higher than that of the control in the whole feeding period, while complement component 4 (C4) level of the probiotic group is significantly higher than that of the control at Day 60. The IgM level of probiotic group is significantly higher than that of control group at Day 30, but no significant difference is observed at Day 60. In conclusion, Psychrobacter sp. SE6 shows good antagonistic activities against potential pathogens and tolerance to mimic gastrointestinal environment, and the dietary administration of Psychrobacter sp. SE6 improves the feed utilization and immune responses of E.coioides.
- Psychrobacter sp. /
- Epinephelus coioides /
- antibacterial activity /
- growth performance /
- immunity

HTML全文

参考文献(26)

覃映雪,池信才,苏永全,等. 网箱养殖青石斑鱼的溃疡病病原[J]. 水产学报,2004,28 (3):297-302.

MERRIFILD D L,DIMITROGLOU A,FOEY A,et al. The current status and future focus of probiotic and prebiotic applications for salmonids[J]. Aquaculture,2010,302:1-18.

樊海平,曾占壮,林煜,等. 养殖鳗鲡肠道益生菌的筛选[J]. 水产学报,2006,30(1):97-102.

NAYAK SK. Probiotics and immunity:a fish perspective[J]. Fish Shellfish Immunol,2010,29:2-14.

NAYAK SK. Role of gastrointestinal microbiota in fish[J]. Aquac Res,2010,41:1553-1573.

SUN Y Z,YANG H L,LING Z C,et al. Gut microbiota of fast and slow growing grouper Epinephelus coioides[J]. Afr J Microbiol Res,2009,3:713-720.

杨红玲,孙云章,马如龙,等. 斜带石斑鱼(Epinephelus coioides)肠道乳酸菌MM1和MM4抑菌特性研究[J]. 海洋与湖沼,2010,41(4):543-548.

孙云章,杨红玲,马如龙,等. 斜带石斑鱼消化道乳酸菌在模拟胃肠道环境中的存活[J]. 中国水产科学,2010,17(1):128-135.

王福强.牙鲆肠道益生菌的分离鉴定及其应用研究 .北京:中国农业大学,2004.

郭国军,覃映雪,陈强,等.大黄鱼病原副溶血弧菌拮抗菌的筛选[J].海洋学报,2008,30(1):127-134.

林浩然.鱼类生理学[M].广州:广东高等教育出版社,1999:35-50.

HYRONIMUS B,LE MARREC C,SASSI A H,et al. Acid and bile tolerance of spore-forming lactic acid bacteria[J]. Int J Food Microbiol,2000,61:193-197.

杨桂梅,张永红,苏娜,等. 2株猪源益生性肠球菌对酸和胆盐及热的耐受性研究[J].北京农学院学报,2008,23(4):29-32.

ERKKIAS S,PETAJA E.Screening of commercial meant starter cultures at low pH and in the presence of bile salts for potential probiotic use[J].Meat Sci,2002,55:297-300.

SON V M,CHANG C C,WU M C,et al. Dietary administration of the probiotic,Lactobacillus plantarum,enhanced the growth,innate immune responses,and disease resistance of the grouper Epinephelus coioides[J]. Fish Shellfish Immunol,2009,26:691-698.

SUN Y Z,YANG H L,MA R L,et al. Probiotic applications of two dominant gut Bacillus strains with antagonistic activity improved the growth performance and immune responses of grouper Epinephelus coioides[J]. Fish Shellfish Immunol,2010,29(5):803-809.

华雪铭,周洪琪,张冬青,等. 多糖和益生菌对暗纹东方鲀免疫功能的调节[J]. 水产学报,2006,30(2):230-235.

CAIPANG C M A,BRINCHMANN M F,KIRON V. Antagonistic activity of bacterial isolates from intestinal microbiota of Atlantic cod,Gadus morhua,and an investigation of their immunomodulatory capabilities[J]. Aquac Res,2010,41:249-256.

LAZADO C C,CAIPANG C M A,GALLAGE S,et al. Expression profiles of genes associated with immune response and oxidative stress in Atlantic cod,Gadus morhua head kidney leukocytes modulated by live and heat-inactivated intestinal bacteria[J]. Comp Biochem Physiol B. Biochem Mol Biol,2010,155:249-255.

BALCáZAR J L,DE BLAS I,RUIZ-ZARZUELA I,et al. Enhancement of the immune response and protection induced by probiotic lactic acid bacteria against furunculosis in rainbow trout (Oncorhynchus mykiss)[J]. FEMS Immunol Med Microbiol,2007,51:185-193.

PANIGRAHI A,KIRON V,PUANGKAEW J,et al. The viability of probiotic bacteria as a factor influencing the immune response in rainbow trout Oncorhynchus mykiss[J]. Aquaculture,2005,243:241-254.

MERRIFIELD D L,DIMITROGLOU A,BRADLEY G,et al. Probiotic applications for rainbow trout(Oncorhynchus mykiss Walbaum):I.Effects on growth performance,feed utilization,intestinal microbiota and related health criteria[J]. Aquac Nutr,2010,16:504-510.

HUANG X X,ZHOU H Q,ZHANG H. The effect of Sargassum fusiforme polysaccharide extracts on vibriosis resistance and immune activity of the shrimp,Fenneropenaeus chinensis[J]. Fish Shellfish Immunol,2006,20:750-757.

SAKAI M. Current research status of fish immunosimulants[J]. Aquaculture,1999,172:63-92.

BRICHNELL I,DALMO R A. The use of immunostimulants in fish larval aquaculture[J]. Fish Shellfish Immunol,2005,19:457-472.

BAGNI M,ROMANO N,FNOIA M G,et al. Short- and long-term effects of a dietary yeast β-glucan (macrogard) and alginic acid (ergosan) preparation on immune response in sea bass(Dicentrarchus labrax)[J].Fish Shellfish Immunol,2005,18:311-325.

施引文献

资源附件(0)

访问统计