海水中一氧化氮的化学发光法测定

丰卫华; 刘春颖; 杨桂朋

海水中一氧化氮的化学发光法测定

中国海洋大学化学化工学院海洋化学理论与工程技术教育部重点实验室,山东青岛266100

基金项目: 国家自然科学基金 (41176062,40706040,40376022);山东省中青年科学家科研奖励基金(2007BS08015);山东省自然科学基金(Y2008E04);教育部"长江学者"奖励计划;山东省"泰山学者"建设工程资助项目。

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出版历程
- 收稿日期: 2010-11-01
- 修回日期: 2011-05-08

Chemiluminescence detection of nitric oxide in seawater

Key Laboratory of Marine Chemistry Theory and Technology,Ministry of Education,College of Chemistry and Chemical Engineering,Ocean University of China,Qingdao 266100,China

摘要

摘要: 基于鲁米诺-H₂O₂混合溶液作为发光液与海水中NO发生反应产生发光信号的原理,提出了一种测定海水中痕量NO的化学发光法。确定了化学发光法测定NO的最佳条件: pH 9.2、浓度1×10^-2 mol/dm³的碳酸盐为缓冲体系,鲁米诺浓度为8.4×10^-5 mol/dm³,H₂O₂ 浓度为1×10^-2 mol/dm³,测定温度为20 ℃。该方法在最佳实验条件下,当NO浓度在1.4×10^-12 ~ 9.8×10^-10 mol/dm³范围内,其浓度值与发光峰面积呈良好的线性关系,相关系数R=0.997,P<0.000 1。该方法的检出限为1.4×10^-12 mol/dm³ (S/N=3),相对标准偏差(RSD)为13.6%(1.2×10^-11 mol/dm³)。采用该方法对胶州湾表层海水中NO的分布进行了初步研究,结果表明,NO浓度范围为未检出~7.1×10^-11 mol/dm³,平均浓度为(1.6±1.8)×10^-11 mol/dm³。
- 一氧化氮(NO) /
- 化学发光法 /
- 海水 /
- 鲁米诺-H₂O₂ /
- 胶州湾
Abstract: A chemiluminescence method measuring the concentration of NO in seawater was established in this paper on the base of a reaction between the mixed solution of luminol-H₂O₂, the chemiluminescence probe, and NO, in which the chemiluminescence signal was emitted. The optimal results in the determination of NO concentration were under the conditions of a pH 9.2 buffer system with 1×10^-2 mol/dm³ carbonate, 8.4×10^-5 mol/dm³ luminol, 1×10^-2 mol/dm³ H₂O₂, and under 20 ℃ and 1×10^-3 mol/dm³ EDTA as a optimal masking agent. Under these optimal conditions, there was a significant linear relationship between the NO concentration and the peak area of chemiluminescence signal in the range of NO concentration from 1.4×10^-12 to 9.8×10^-10 mol/ dm³ (R=0.997, P <0.000 1). The detection limit for NO was 1.4×10^-12 mol/dm³ (S/N). In 7 times of parallel determination to 1.2×10^-11 mol/dm³NO aqueous solution, the relative standard deviation (RSD) was 13.6%. The NO concentration in the surface seawater of Jiaozhou Bay, China was successfully determined using this method and the results indicated that the concentrations of NO ranged from undetected to 7.1×10^-11 mol/ dm³ with an average of (1.6±1.8)×10^-11 mol/dm³.
- nitric oxide (NO) /
- chemiluminescence /
- seawater /
- luminol-H₂O₂ /
- Jiaozhou Bay

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