基于叶绿素荧光技术的紫菜光适应特征研究

张涛; 沈宗根; 姚春燕; 陆勤勤; 姜红霞; 朱建一; 许璞

基于叶绿素荧光技术的紫菜光适应特征研究

1.
常熟理工学院应用技术研究院,江苏常熟 215500
2.
常熟理工学院应用技术研究院,江苏常熟 215500;南京师范大学生命科学学院,江苏南京 210097
3.
江苏省海洋水产研究所,江苏南通 226007

基金项目: 农业部公益性行业专项(200903030-D);"十一五"国家支撑项目(2006BAD09A08-05);江苏省高技术研究项目(BG2006333);国家自然科学基金(31070165)。

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出版历程
- 收稿日期: 2009-09-06

Light adaptation characteristics of porphyra studied by chlorophyll fluorescence technology

1.
Department of Biology, Changshu Institute of Technology, Changshu 215500,China
2.
Department of Biology, Changshu Institute of Technology, Changshu 215500,China;College of Life Science, Nanjing Normal University, Nanjing 210097, China
3.
Marine Fisheries Institute of Jiangsu Province, Nantong 226007, China

摘要

摘要: 通过水样调制式叶绿素荧光仪,对坛紫菜、条斑紫菜叶状体和丝状体在不同光照条件下叶绿素荧光特性进行研究。结果表明:紫菜叶状体和丝状体实际量子效率在光照处理后逐步下降,且随着处理光强的上升,实际量子效率的下降速率更为明显。快速光曲线初始斜率(α)结果与实际量子效率相似,表明光照时间的延长以及光照强度的升高均引起样品实际光能利用效率的下降。快速光曲线结果也显示样品半饱和光强低于150 μmol/(m²·s),说明紫菜这类海藻偏向较低光强的适应性。随着处理光强的上升,样品I_k值逐步升高,说明紫菜具备适应光强变化的光能调节机制。方差分析结果表明,两种紫菜丝状体实际量子效率、快速光曲线初始斜率(α)和最大相对电子传递速率均因光强的上升而显著降低(P＜0.05),叶状体实际量子效率、快速光曲线初始斜率也随光强上升而下降,但最大相对电子传递速率基本维持不变,表明紫菜两个生长世代的藻体对光强具有不同的响应机制。诱导曲线测定结果表明,经强光适应的紫菜叶状体光化学猝灭较高,而非光化学猝灭较低,显示强光处理能够提高叶状体对光强的适应能力。
- 紫菜 /
- 光强 /
- 叶绿素荧光 /
- 光能利用效率
Abstract: The chlorophyll fluorescence characteristics of thallus and conchocelis in both species of Porphyra grown under different light intensities were investigated with Water-PAM chlorophyll fluorometer. Results showed that the effective quantum yield of PSII (Yield) of Porphyra decreased after the treatment, and the effective quantum yield of sample decreased obviously with the increasing of light intensity. Results of the initial slop of rapid light curve (α) are similar with the effective quantum yield of sample, which meant both the time after treatment and the increasing of light intensities Would induce the decreasing of actual light energy use efficiency of Porphyra. The minimum saturating irradiance (I_k) calculated from the rapid light curves (RLC) of thallus and conchocelis in Porphyr were less than 150 μmol·m^-2·s^-1, which indicates Porphyra was one kind of seaweed adapted to low light intensity. High PAR increased the I_k of Porphyra,which supported the idea that Porphyra had light energy regulatory mechanism to adapt to the changes of light intensities. ANOVA results showed that the effective quantum yield of PSII, the initial slope of rapid light curve (α), the maximum relative electron transport rate (r_{ETR_max}) of conchocelis in both species of Porphyra decreased significantly with the increasing of light intensities(P<0.05), the effective quantum yield of PSII and the initial slope of rapid light curve of thallus also decreased with the increasing of light intensity, but the maximum relative electron transport rate had little change, which meant conchocelis and thallus of Porphyra might have different response mechanisms to the changes of light intensities. The results of induce curve indicated that thallus of both species of Porphyra grown under high light intensity had higher photochemical quenching (qP) and lower non-photochemical quenching (NPQ) than those grown under low light intensity, which suggested that the light tolerance of thallus of Porphyra could be improved by high light intensity acclimation.
- Porphyra /
- light intensity /
- chlorophyll fluorescence /
- light use efficiency

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参考文献(35)

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