缘管浒苔和羽藻氮、磷营养生理学研究
Nitrogen and phosphorus nutrition physiology of Enteromorpha linza and Bryopsis plumosa
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摘要: 以无性繁殖系为材料,开展了缘管浒苔和羽藻对NO3-,NH4+和PO43-的吸收动力学、生长动力学研究。吸收动力学研究结果表明缘管浒苔和羽藻对NO3- 和PO43-的吸收方式为主动运输,对NH4+的吸收方式为被动扩散。缘管浒苔对NO3-的最大吸收速率(Vmax)、对NH4+的吸收斜率都大于羽藻,说明缘管浒苔对高浓度的NO3-和NH4+具有更强的吸收能力。缘管浒苔吸收NO3-和NH4+的a值远大于羽藻,说明在低营养盐浓度时,缘管浒苔对NO3-和NH4+的亲和力更强。在PO43-的吸收中,羽藻的最大吸收速率(Vmax)远大于缘管浒苔,说明羽藻对高浓度PO43-的吸收能力更强,但缘管浒苔的a值远大于羽藻,说明前者在低营养盐浓度时PO43-的亲和力更强。生长动力学研究结果表明,硝酸氮是促进两种海藻快速生长的最适宜氮源形式,氨氮更易促进藻体叶绿素的积累。在相同氮营养条件下,羽藻表现出比缘管浒苔更强的生长优势。Abstract: The studies on the uptake and growth kinetics of NO-3,NH+4和PO3-4N in the clone of Enteromorpha linza and Bryopsis plumosa were conducted. The results suggested that NO-3 and PO3-4 were taken up in the form of active uptake by the two alga, with NH+4 taken up by passive diffusion. The parameters of Vmax and straight slope for E linza were higher than that for B. plumose, which suggested that E. linza could be more favor to high N than B. plumosa. Moreover, the value of a (Vmax/Ks) for NO-3 and NH+4 in E. linza was obviously higher than that in B. plumosa, which showed that E. linza could have more approachability to low N than B. plumosa. As for the uptaken of PO3-4, E. linza may have higher P uptake capacities at high P and lower approachability to low P than B. plumosa due to the higher Vmax and lower a than B. plumosa. NO-3 is the most favorite N form to meet the growth for both species, while NH+4 is more object to increase the accumulation of chlorophyll than NO-3. In addition, B. plumosa exhibited higher growth rate than E. linza under same N condition.
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Key words:
- Enteromorpha linza /
- Bryopsis plumose /
- NO3- /
- NH4+ /
- PO43- /
- uptake kinetics /
- growth kinetics
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LAPOINTE B E, BARILE P J. MATZIE W R. Anthropogenic nutrient enrichment of seagrass and coral reef communities in the Lower Florida Keys: discrimination of local versus regional nitrogen sources[J]. J Exp Mar Biol Ecol,2004, 308(1), 23-58. LAPOINTE B E, BARILE P J, LITTLER M M, et al. Macroalgal blooms on southeast Florida coral reefs: Ⅰ. Nutrient stoichiometry of the invasive green alga Codium isthmocladum in the wider Caribbean indicates nutrient enrichment[J]. Harmful Algae, 2005, 4: 1092-1105. LAPOINTE B E, BARILE P J, LITTLER M M, et al. Macroalgal blooms on southeast Florida coral reefs: Ⅱ. Cross-shelf discrimination of nitrogen sources indicates widespread assimilation of sewage nitrogen[J]. Harmful Algae, 2005, 4: 1106-1122. LAPOINTE B E, BEFORD B J. Drift Rhodophyte blooms emerge in Lee County, Florida, USA: Evidence of escalating coastal eutrophication[J]. Harmful Algae, 2007, 6:421-437. 杨宇峰,费修绠. 大型海藻对富营养化海水养殖区生物修复的研究与展望[J]. 青岛海洋大学学报,2003,33(1):53-57. VALIELA I, MCCLELLAND J, HAUXWELL J, et al. Macroalgal blooms in shallow estuaries: controls and ecophysiological and ecosystem consequences[J]. Limnol Oceanogr, 1997, 42:1105-1118. ROSENBURG G, RAMUS J. Uptake of inorganic nitrogen and seaweed surface area: volume ratios[J]. Aquat Bot, 1984, 19: 65-72. FUGITA R M. The role of nitrogen status in regulating transient ammonium uptake and nitrogen storage by macroalgae[J]. J Exp Mar Biol Ecol, 1985, 92:283-301. NEILL G, BARR T, REES A V. Nitrogen status and metabolism in the green seaweed Enteromorpha intestinalis: an examination of three natural populations[J]. Mar Ecol Prog Ser, 2003, 249: 133-144. TAYLOR M W, REES A V. Kinetics of ammonium assimilation in two seaweeds Enteromorpha sp. and Osmundaria colensoi (Rhodophyceae) [J]. J Phycol,1999, 35: 740-746. PEGGY F, FONG J J, FONG C R. Growth, nutrient storage, and release of dissolved organic nitrogen by Enteromorpha intestinalis in response to pulses of nitrogen and phosphorus [J]. Aquat Bot, 2004, 78: 83-95. COHEN R A, FONG P. Nitrogen uptake and assimilation in Enteromorpha intestinalis (L.) Link (Chlorophyta): using 15N to determine preference during simultaneous pulses of nitrate and ammonium[J]. Journal of Experimental Marine Biology and Ecology, 2004, 309: 67- 77. 杨敏文. 快速测定植物叶片叶绿素含量方法的探讨[J].光谱实验室,2002,19(4):478-481. 李合生. 植物生理生化实验原理和技术[M]. 北京:高等教育出版社,2004:199-201. HAINES K C, WHEELER PA. Ammonium and nitrate uptake by the marine macrophytes Hypnea musciformis (Rhodophyta)and Macrocystis pyrifera (Phaeophyta)[J]. J Phycol, 1978, 14:319-24. WALLENTINUS I. Comparisons of nutrient uptake rates for Baltic macroalgae with different thallus morphologies[J]. Mar Biol, 1984, 80:215-25. BRAGA A C, YONESHIGUE V Y. Nitrogen and phosphorus uptake by the Brazilian kelp Laminaria abyssalis (Phaeophyta) in culture[J]. Hydrobiologia, 1996, 326/327:445-50. THOMAS T E, HARRISON P J. Effect of nitrogen supply on nitrogen uptake accumulation and assimilation in Porphyra perforata (Rhodophyta) [J]. Mar Biol, 1985, 85:269-78. TOPINKA J A. Nitrogen uptake by Fucus spiralis (Phaeophyceae) [J]. J Phycol, 1978, 4:241-247. 徐永健,钱鲁闽,韦玮,等. 两种大型海藻自然环境下的营养动力学研究[J].海洋环境科学,2007,26(2):161-165. KAREN J, MCGLATHERY, PEDERSEN M F, et al. Changes in intracellular nitrogen pools and feedback controls on nitrogen uptake in Chaetomorpha linum (Chlorophyta) [J]. J Phycol, 1996, 32:393-401. FUJITA R M. Metabolic regulation of ammonium uptake by Ulva rigida: a compartmental analysis of the fate limiting step for uptake[J]. J Phycol, 1988, 24 :560-566. 邵魁双. 海藻无性系的构建 .青岛:中国科学院海洋研究所.2003,88-90. 张乃星. 过量氮和磷引起的富营养化对海水无机碳源汇强度的影响 .青岛:中国科学院海洋研究所, 2008, 64-80. 刘静雯,董双林. 海藻的营养代谢及其对主要营养盐的吸收动力学[J]. 植物生理学通讯, 2001, 37(4): 325-330. 徐永健, 陆开宏, 管保军. 不同氮磷浓度及氮磷比对龙须菜生长和琼胶含量的影响[J]. 农业工程学报, 2006, 22(8): 209-213. 黄鹤忠,孙菊燕,申华,等. 无机氮浓度及其配比对细基江蓠繁枝变型生长及生化组成的影响[J]. 海洋科学, 2006, 30(9): 23-27. PEDERSEN M F, BORUM J. Nutrient control of estuarine macroalgae: growth strategy and the balance between nitrogen requirements and uptake[J]. Mar Ecol Prog Ser, 1997, 161: 155-163. UTPAL N, BRIAN C, CRAWFORD W, et al. Genetic control of surface curvature[J]. Science, 2003, 299:1404-1407. AQUIRRE-LIPPERHEIDE M, ESTRADA-RODRIGUEZ F J, EVANS L V. Facts, problems and needs in seaweed tissue culture: an appraisal[J]. J Phycol, 1995,31:677-688. JONES A B, DENNISON W C, STEWART G R. Influence of nitrogen source and availability on amino acids pigments and tissue nitrogen of Gracilaria eduils (Rhodophyta) [J]. J Phycol, 1996, 32: 757-766.
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