凡纳滨对虾α-淀粉酶基因PCR-RFLP多态性与生长性状的相关分析

辛静静; 刘小林; 李喜莲; 王宪宗; 黄皓; 相建海

凡纳滨对虾α-淀粉酶基因PCR-RFLP多态性与生长性状的相关分析

1.
西北农林科技大学动物科技学院陕西省农业分子生物学重点实验室,陕西杨凌 712100
2.
海南南疆生物技术有限公司,海南三亚 572000
3.
中国科学院海洋研究所实验海洋生物学开放实验室,山东青岛 266071

基金项目: 国家"十一五""863"重点项目(2006AA10A406);中国科学院实验海洋生物学开放课题。

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出版历程
- 收稿日期: 2010-08-09

PCR-RFLP polymorphism of alpha-amylase gene and its association with growth traits of Litopenaeus vannamei

1.
College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling 712100, China
2.
Nanjiang Marine Biotechnology Company Limited, Sanya 572000, China
3.
Experimental Marine Biology Laboratory, Institute of Oceanology, Chinese Academy of Sciences,Qingdao 266071, China

摘要

摘要: 采用PCR-RFLP法对379尾凡纳滨对虾α-淀粉酶基因(AMY)多态性进行检测,并分析AMY基因多态性与生长性状的相关性。结果表明:引物AMY-5与AMY-6扩增片段均具多态性,引物AMY-5扩增产物有CC, CT和TT 3种基因型,AMY-6扩增产物有AA, AB和BB 3种基因型。AMY基因AMY-5引物位点TT基因型与CC基因型相比在外显子5的123 bp处发生C→T突变,AMY-6引物位点BB基因型与AA基因型相比在外显子6的81 bp处发生C→T突变,2处突变均未引起氨基酸变化,表现为沉默突变。最小二乘法分析结果表明,CC, CT基因型在体重、体长、头胸甲长、头胸甲宽、第一腹节长及尾节长6个生长指标上均显著高于TT基因型(P＜0.05)。AA, BB基因型在体长、第一腹节长及尾节长3个指标上显著高于AB基因型(P＜0.05),在体重、头胸甲长及头胸甲宽指标上AA基因型显著高于AB基因型(P＜0.05)。结论:AMY基因多态性对凡纳滨对虾生长性状有显著影响,可以作为影响凡纳滨对虾生长性状的候选基因,为凡纳滨对虾选育提供分子遗传标记。
- 凡纳滨对虾 /
- α-淀粉酶基因 /
- PCR-RFLP /
- 生长性状
Abstract: The polymorphism of the alpha-amylase (AMY) gene was analyzed by PCR-RFLP method in 379 Litopenaeus vannamei, and association analyses were carried out to evaluate the effects of genotypes of the candidate genes on growth traits in Litopenaeus vannamei. The results showed that both the products amplified by primer AMY-5 and AMY-6 displayed polymorphisms, and there were three genotypes (CC, TT and CT) for AMY-5 and three genotypes (AA, AB and BB) for AMY-6 respectively. The results indicated that there was one single nucleotide mutation (C→T) in the exon5 (123 bp) and one single nucleotide mutation (C→T) in the exon6 (81 bp) of the alpha-amylase gene. The two mutations did not cause any amino acid changes and were silence mutations. Least square was applied to analyze the correlation of different genotypes and the growth traits. The CC and CT genotypes showed significant differences in body mass, body length, carapace length, carapace width, first abdominal segment length and the last abdominal segment length compared with the genotype TT(P<0.05). The AA and BB genotypes showed significant differences in body length, first abdominal segment length and the last abdominal segment length compared with the genotype AB (P<0.05). The AA genotype showed significant differences in body mass, carapace length, carapace width compared with the genotype AB (P<0.05). It implied that the AMY gene could have a major effect on the growth traits of Litopenaeus vannamei, and could be used as the gene that significantly affect the growth traits in Litopenaeus vannamei, and these molecular genetic markers may be applied for Litopenaeus vannamei breeding.
- Litopenaeus vannamei /
- alpha-amylase gene /
- PCR-RFLP /
- growth traits

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参考文献(21)

周鑫.南美白对虾的苗种繁育与淡水养殖技术[J].科学养鱼, 2003: 1-15.

张存善,李色东,王亚平,等. 2个凡纳滨对虾群体仔虾的生长比较[J].水产科学,2010,29(06): 325-328.

吴立峰,张吕平,沈琪,等. 凡纳滨对虾不同家系的形态性状对体重的影响[J].海洋湖沼通报, 2010(02): 37-48.

SCHULER D J, BOARDMAN G D, KUHN D D, et al. Acute toxicity of ammonia and nitrite to Pacific white shrimp, Litopenaeus vannamei, at low salinities[J]. Journal of the World Aquaculture Society, 2010, 41(3): 438-446.

SENAPIN S, THAOWBUT Y, GANGNONNGIW W, et al. Impact of yellow head virus outbreaks in the white leg shrimp, Penaeus vannamei (Boone), in Thailand[J]. Journal of Fish Diseases, 2010, 33(5): 421-430.

LI C C, YEH S T, CHEN J C. Innate immunity of the white shrimp Litopenaeus vannamei weakened by the combination of a Vibrio alginolyticus injection and low-salinity stress[J]. Fish & Shellfish Immunology, 2010, 28(1): 121-127.

陈昌生,邹春平,纪德华,等. 延迟投饵对南美白对虾状幼体存活、变态和生长的影响[J].海洋学报, 2003, 25(Supp2): 208-213.

ZENG D, CHEN X, LI Y, et al. Analysis of Hsp70 in Litopenaeus vannamei and detection of SNPs[J]. Crustacean Biology, 2008, 28(4): 727-730.

VALLES-JIMENEZ R, CRUZ P, PEREZ-ENRIQUEZ R. Population genetic structure of Pacific white shrimp (Litopenaeus vannamei) from Mexico to Panama: microsatellite DNA variation[J]. Mar Biotechnol (NY), 2004, 6(5): 475-484.

MOORE S S, WHAN V, DAVIS G P, et al. The development and application of genetic markers for the Kuruma prawn Penaeus japonicus[J]. Aquaculture, 1999, 173: 19-32.

ZHANG L, YANG C, ZHANG Y, et al. A genetic linkage map of Pacific white shrimp (Litopenaeus vannamei): sex-linked microsatellite markers and high recombination rates[J]. Genetica, 2006, 131(1): 37-49.

DU Z Q, CIOBANU D C, ONTERU S K, et al. A gene-based SNP linkage map for pacific white shrimp, Litopenaeus vannamei[J]. Animal Genetics, 2010, 41(3): 286-294.

钱海丰,赵晓娟,赵心爱.α-淀粉酶基因表达的调控[J].西北农业学报, 2003, 12(4): 87-90.

崔锦,马向东. 淀粉酶基因的多样性[J]. 郑州牧业工程高等专科学校学报, 2009, 29(02): 21-23.

黄健华,贾世海,张勇,等. 家蚕α淀粉酶基因的多态性研究[J].中国农业科学, 2006,39(11): 2390-2394.

GLENN K L, GRAPES L, SUWANASOPEE T, et al. SNP analysis of AMY2 and CTSL genes in Litopenaeus vannamei and Penaeus monodon shrimp[J]. Animal Genetics, 2005, 36(3): 235-236.

ALJANABI S M, MARTINEZ I. Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques[J]. Nucleic Acids Research, 1997, 25(22): 4692-4693.

VAIMA D, MERCIER D, MOAZAMIGOUDARZI K, et al. A set of 99 cattle microsatellites- characterization, synteny mapping, and polymorphism[J]. Mammalian Genome, 1994, 5(5): 288-297.

彭敏,陈晓汉,曾地刚,等. 凡纳滨对虾α-淀粉酶基因的SNPs检测[J].水产科学, 2008, 27(07): 363-365.

HUVET A, JEFFROY F, FABIOUX C, et al. Association among growth, food consumption-related traits and amylase gene polymorphism in the Pacific oyster Crassostrea gigas[J]. Animal Genetics, 2008, 39(6): 662-665.

PRUDENCE M, MOAL J, BOUDRY P, et al. An amylase gene polymorphism is associated with growth differences in the Pacific cupped oyster Crassostrea gigas[J]. Animal Genetics, 2006, 37(4): 348-351.

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