2008年夏季白令海陆架区微微型浮游植物分布及环境相关性分析

张芳; 何剑锋; 林凌; 光应芝; 马玉欣

2008年夏季白令海陆架区微微型浮游植物分布及环境相关性分析

1.
中国极地研究中心国家海洋局极地科学重点实验室,上海 200136
2.
中国极地研究中心国家海洋局极地科学重点实验室,上海 200136;华东师范大学生命科学学院,上海 200241
3.
中国极地研究中心国家海洋局极地科学重点实验室,上海 200136;中国海洋大学化学化工学院,山东青岛 266100
4.
中国极地研究中心国家海洋局极地科学重点实验室,上海 200136;同济大学环境科学与工程学院,上海 200136

基金项目: 国家自然科学基金(41076130);中国极地科学战略研究基金项目(2008209);极地科学青年创新基金项目(JDQ200802);国家海洋局青年基金项目(2010116);国家海洋局海洋生态系统与生物地球化学重点实验室开放研究基金(LMEB200902)。

计量
- 文章访问数: 1671
- HTML全文浏览量: 2
- PDF下载量: 1455
- 被引次数: 0
出版历程
- 收稿日期: 2010-08-06

Distribution of picophytoplankton and environmental correlation analysis in Bering Sea shelf during the summer of 2008

1.
State Oceanic Administration Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai 200136, China
2.
State Oceanic Administration Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai 200136, China;College of Life Science, East China Normal University, Shanghai 200062, China
3.
State Oceanic Administration Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai 200136, China;College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China
4.
State Oceanic Administration Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai 200136, China;School of Environmental Science and Engineering, Tongji University, Shanghai 200092, China

摘要

摘要: 利用2008年夏季我国第3次北极科学考察资料,基于流式细胞技术,对白令海北部陆架区的微微型浮游植物丰度、细胞大小(碳含量)、色素浓度的分布特征进行了分析,并对该类群的环境适应性进行了研究。结果表明,微微型浮游植物中仅含聚球藻和真核藻,其丰度范围分别为0.14×10⁶ 2.69×10⁶和0.23×10⁶-12.49×10⁶个/dm³。聚球藻的叶绿素a和藻红蛋白含量、微微型真核藻的叶绿素a含量与类群丰度以及微微型真核藻的类胡萝卜素含量与细胞大小间均存在同向变化趋势。两类藻偏向于喜温嗜淡型,更适合在寡营养环境中保持较高的丰度,但能在高营养盐浓度下形成相对较高的碳含量。越接近陆地,细胞越小,丰度越大,碳含量及FL2/FL3越低;所处层位越深、纬度越高,则细胞越大,碳含量及FL2/FL3越高。北极气温升高和径流量的增加有利于陆架区微微型浮游植物类群丰度的增加。
- 白令海 /
- 微微型浮游植物 /
- 流式细胞术 /
- 环境相关性
Abstract: The abundance, cell size (cellular carbon content), and cellular pigments of picophytoplankton on the shelf area of the north Bearing Sea(61°29' 64°21'N, 168°00' 174°32'W) were analyzed by flow cytometry(FCM).A statistical analysis was used to study the response of the picophytoplankton to environmental changes. Synechococcus and picoeukaryotes were the only type of the picophytoplankton community; and their respective cell abundance was 0.01×10⁶ 2.69×10⁶ and 0.47×10⁶ 13.20×10⁶ cells/dm³. The chlorophyll a, phycoerythrin, and the cell size (cellular carbon content) had the same changing trend with the change of environmental factors. Comparatively, the chlorophylls a and the cell abundance, and the carotenoids and the cell size (cellular carbon content) of the picoeukaryotes had the same changing trends, respectively. Both salinity and nutrients had significant influence on the cellular carbon content and the ratio of carotinoids to chlorophylls a of picoeukaryotes. High nutrient concentration was prone to induce high ratio of cellular carbon content to chlorophylls a. Both synechococcus and picoeukaryotes preferred relatively high temperature and low salinity; and were easily to form high cell abundance in oligotrophic water, whereas in seawaters with relatively high nutrient concentration were prone to form cells with high cellular carbon content.The cell abundance of picoeukaryotes decreased intensely when the nitrogen-phosphorus ratio greater than 7. The cell abundance was relatively high, whereas the cell size (cellular carbon content) and the phycoerythrin-chlorophyll a ratio were relatively small. Comparatively, the cell size (cellular carbon content) and the phycoerythrin-chlorophyll a ratio were relatively large and the cell abundance was relatively low when the picophytoplankton was at relatively deep water layers and high latitudes. The ascending of the water temperature and the increase of the inflows of freshwater from the continent can increase both the abundance and the variety of picophytoplankton on the continental shelf of the north Bering Sea.
- Bering Sea /
- picophytoplankton /
- flow cytometry /
- environmental relationship

HTML全文

参考文献(26)

CAMPBELL L, NOLLA H A, VAULOT D. The importance of prochlorococcus to community structure in the central North Pacific Ocean [J]. Limnol Oceanogr, 1994, 39(4): 954—961.

HOFFMANN L J, PEEKEN I, LOCOCHE K, et al. Different reactions of Southern Ocean phytoplankton size classes to iron fertilization[J]. Limnol Oceanogr, 2006, 51(3): 1217—1229.

ZHANG Y, JIAO N, HONG N. Comparative study of picoplankton biomass and community structure in different provinces from subarctic to subtropical oceans [J]. Deep-Sea Res II, 2008, 55(14-15): 1605—1614.

WALERON M, WALERON K, VINCENT W F, et al. Allochthonous inputs of riverine picocyanobacteria to coastal waters in the Arctic Ocean [J]. FEMS Microbiol Ecol, 2007, 59(2): 356—365.

HOFSTAAT J W, DE VREEZE M E J, VAN ZEIJL W J M, et al. Flow cytometric discrimination of phytoplankton classes by fluorescence emission and excitation properties [J]. J Fluorescence, 1991, 1: 249—265.

OLSON R J, ZETTLER E R, ANDERSON O K. Discrimination of eukaryotic phytoplankton cell types from light scatter and autofluorescence properties measured by flow cytometry [J]. Cytometry, 1989, 10(5): 636—643.

LIU H, SUZUKI K, MINAMI C, et al. Picoplankton community structure in the subarctic Pacific Ocean and the Bering Sea during summer 1999 [J]. Mar Ecol Prog Ser, 2002, 237(3): 1—14.

GUTIUTIRREZ-RODRIUEZ A, LATASA M, ESTRADA M, et al. Carbon fluxes through major phytoplankton groups during the spring bloom and post-bloom in the northwestern Mediterranean Sea [J]. Deep-Sea Res: I, 2010, 57(4): 486—500.

NETTO A T, CAMPOSTRINI E, OLIVEIRA J, et al. Photosynthetic pigments, nitrogen, chlorophyll a fluorescence and SPAD-502 readings in coffee leaves [J]. Scientia Horticulturae, 2005, 104(2): 199—209.

MENDOZA H, DE LA JARA A, FREIJANES K, et al. Characterization of Dunaliella salina strains by flow cytometry: a new approach to select carotenoid hyperproducing strains [J]. Electronic J Biotech, 2008, 11(4): 1—13.

CELLAMARE M, ROLLAND A, JACQUET S. Flow cytometry sorting of freshwater phytoplankton [J]. J Appl Phycol, 2010, 22(1): 87—100.

ZHANG F, SU R, HE J, et al. Identifying phytoplankton in seawater based on discrete excitation-emission fluorescence spectra [J]. J Phycol, 2010, 46(2): 403—411.

GRASSHOFF K, EHRHARDT M, KREMING K.Methods of Seawater Analysis 3ed[M].Germany Wainheins.1999:600.

BALFOORT H W, SNOEK J, SMITS J R M, et al.Automatic identification of algae: Neural network analysis of flow cytometric data[J].J Plankton Res.1992,14(4): 575—589.

ZAKHIA F, JUNGLBUT A-D, TATON A, et al.Cyanobacteria in cold ecosystems[M]//MARGESIN R, et al.Psychrophiles: from Biodiversity to Biotechnology.Berlin Springer-Verlag.2008: 121—134.

ANTONIADES D, DOUGLAS M S V, SMOL J P. Benthic diatom autecology and inference model development from the Canadian High Arctic Archipelago [J]. J Phycol, 41(1): 30—45.

SAINDON D. Quantifying the magnitude of nutrient limitation on phytoplankton in Kings Bay, Florida, USA .University of Florida, 2005.

BEUTLER M, WILSHIRE K H, MEYER B, et al. A fluorometric method for the differentiation of algal populations in vivo and in situ [J]. Photosyn Res, 2002, 72(1): 39—53.

YENTSCH C S, PHINNEY D A. Spectral fluorescence: an ataxonomic tool for studying the structure of phytoplankton populations [J].J Plankton Res, 1985, 7(5): 617—632.

VINCENT W F, BOWMAN J P, RANKIN L M, et al. Phylogenetic diversity of picocyano-bacteria in arctic and antarctic ecosystems[M]//BELL C, BRYLINSKY M, JOHNSON-GREEN M. 2000: 317—322.

GARNEAU M- , VINCENT W F, ALONSO-S EZ L, et al. Prokaryotic community structure and heterotrophic production in a river-influenced coastal arctic ecosystem [J]. Aquat Microb Ecol, 2006, 42: 27—40.

VELDHUIS M J W, CUCCI T L, SIERACKI M E. Cellular DNA content of marine phytoplankton using two new fluorochromes: taxonomic and ecological implications [J]. J Phycol, 1997, 33(3): 527—541.

VELDHUIS M J W, TIMMERMANS K R, CROOT P W, et al. Picophytoplankton: a comparative study of their biochemical composition and photosynthetic properties [J]. J Sea Res, 2005, 53(1-2): 7—24.

REDFIELD A C. On the proportions of organic derivations in sea water and their relation to the composition of plankton[M]//James Johnstone Memorial Volume.Liverpool: University Press of Liverpool, 1934: 177—192.

HAMILTON A K, LOVEJOY C, GALAND P E, et al. Water masses and biogeography of picoeukaryote assemblages in a cold hydrographically complex system [J]. Limnol Oceanogr, 2008, 53(3): 922—935.

LI W K W, MACLAUGHLIN F A, LOVEJOY C, et al. Smallest algae thrive as the Arctic Ocean freshens[J]. Science, 2009, 326(5952): 539.

施引文献

资源附件(0)

访问统计

点击查看大图

计量

文章访问数: 1671
HTML全文浏览量: 2
PDF下载量: 1455
被引次数: 0

姓名
邮箱
手机号码
标题
留言内容
验证码

留言板