流式细胞术检测脂多糖对罗氏沼虾血细胞活性和免疫功能的影响

冼健安; 王安利; 苗玉涛; 伍娟

流式细胞术检测脂多糖对罗氏沼虾血细胞活性和免疫功能的影响

华南师范大学生命科学学院广东省水产健康安全养殖广东省重点实验室, 广东广州市510631;华南师范大学生命科学院生态与环境科学广东省高等学校重点实验室, 广东广州 510631

基金项目: 国家自然科学基金资助项目(30671628); 广东省教育部产学研结合资助项目(2006D90204005); 国家科技支撑计划项目(2007BAD29B06); 广东省科技计划项目(2007B020708013); 广东省重点科技项目(2009A020101006); 广州市科技支撑计划项目(2009Z1-E681)。

计量
- 文章访问数: 1654
- HTML全文浏览量: 7
- PDF下载量: 1895
- 被引次数: 0
出版历程
- 收稿日期: 2009-04-19

Flow cytometric analysis of viability and immune response of Macrobrachium rosenbergii haemocytes activated by lipopolysaccharide

Key Laboratory of Guangdong Province for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou 510631, China;Key Laboratory of Guorngdong Higher Education for Ecology ornol Environmental Science, College of Life S

摘要

- 脂多糖 /
- 罗氏沼虾 /
- 血细胞 /
- 免疫反应 /
- 流式细胞术
- lipopolysaccharide /
- Macrobrachium rosenbergii /
- haemocyte /
- immune response /
- flow cytometry

HTML全文

参考文献(38)

沈萍. 微生物学[M].北京: 高等教育出版社, 2000.

张顺财. 内毒素基础与临床[M]. 北京:科学出版社, 2003.

BAUCHAU A G. Invertebrate Blood Cells[M]. New York: New York Acad Press, 1981: 386-420.

于建平.日本对虾血细胞分类、密度及组成[J].青岛海洋大学学报, 1993, 23(1): 107-114.

叶燕玲,陈宽智. 中国对虾血细胞超微结构、分类及计数[J].青岛海洋大学学报,1993, 23(2): 35-42.

李光友,王青.中国对虾血细胞及其免疫研究[J].海洋与湖沼, l995, 26(6): 59l-596.

许秀芹,孙虎山,王宜艳.酵母聚糖和甘氨酸锌对栉孔扇贝血细胞的影响[J].上海水产大学学报, 2006, 15(2): 150-155.

SDERHLL K, CERENIUS L.Crustacean immunity[J]. Annual Review of Fish Diseases, 1992, 2: 3-23.

SDERHLL K, CERENIUS L. Role of the prophenoloxidase-activating system in invertebrate immunity[J]. Curr Opin Immunol, 1998, 10: 23-28.

JOHANSSON M W, KEYSER P, SRITUNYALUCKSANA K, et al. Crustacean haemocytes and haemopoiesis[J]. Aquaculture, 2000, 191: 45-52.

SMITH V J, BROWN J H, HAUTON C.Immunostimulation in crustaceans: does it really protect against infection? [J].Fish Shellfish Immunol, 2003, 15: 71-90.

SDERHLL K, HALL L. Lipopolysaccharide-induced activation of prophenoloxidase activating system in crayfish haemocyte lysate[J]. Biochim Biophys Acta, 1984, 797: 99-104.

GOLLAS-GALVN T, HERNNDEZ-LPEZ J, VARGAS-ALBORES F. Effect of calcium on the prophenoloxidase system activation of the brown shrimp (Penaeus californiensis, Holmes) [J]. Comp Biochem Physiol, 1997, 117A: 419-425.

刘恒,李光友.免疫多糖对养殖南美白对虾作用的研究[J].海洋与湖沼, 1998, 29(2): 113-118.

SRITUNYALUCKSANA K, SITHISARN P, WITHAYACHUMNARNKUL B, et al.Activation of prophenoloxidase, agglutinin and antibacterial activity in haemolymph of the black tiger prawn, Penaeus monodon, by immunostimulants[J]. Fish Shellfish Immunol, 1999, 9: 21-30.

汪小锋,樊廷俊,丛日山,等.几种免疫促进剂对中国对虾血细胞数量、形态结构以及酚氧化酶产量和活性的影响[J].水产学报, 2006, 29(1): 66-73.

SMITH V J, SDERHLL K. Induction of degranulation and lysis of haemocytes in the freshwater crayfish, Astacus astacus by components of the prophenoloxidase activating system in vitro[J]. Cell Tissue Res, 1983, 233: 295-303.

JOHANSSON M W, SDERHLL K. Exocytosis of the prophenoloxidase activating system from crayfish haemocytes[J]. J Comp Physiol B, 1985, 156: 175-181.

CRDENAS W, DANKENT J R, JENKINS J A. Flow cytometric analysis of crayfish haemocytes activated by lipopolysaccharides[J]. Fish Shellfish Immunol, 2004, 17: 223-233.

SUNG H H, HWANG S F, TASI F M. Responses of giant freshwater prawn (Macrobrachium rosenbergii) to challenge by two strains of Aeromonas spp.[J]. J Invertebr Pathol, 2000, 76(4): 278-284.

SDERHLL K, SMITH V J, JOHANSSON M W. Exocytosis and uptake of bacteria by isolated haemocyte populations of two crustaceans: evidence for cellular cooperation in the defense reactions of arthropods[J]. Cell Tissue Res, 1986, 245: 43-49.

THRNQVIST P O, JOHANSSON M W, SDERHLL K. Opsonic activity of cell adhesion protein and β-1,3-glucan binding protein from two crustaceans[J]. Dev Comp Immunol, 1994, 18: 3-12.

SOLEM S T, JORGENSEN J B, ROBERTSEN B. Stimulation of respiratory burst and phagocytic activity in Atlantic salmon (Salmo salar L.) macrophages by lipopolysaccharide[J]. Fish Shellfish Immunol, 1995, 5: 475-491.

HERVOUET E, SIMONNET H, GODINOT C. Mitochondria and reactive oxygen species in renal cancer[J]. Biochimie, 2007, 89: 1080-1088.

PIECZENIK S R, NEUSTADT J. Mitochondrial dysfunction and molecular pathways of disease[J]. Exp Mol Pathol, 2007, 83: 84-92.

SULIMAN H B, WELTY-WOLF K E, CARRAWAY M S, et al. Lipopolysaccharide induces oxidative cardiac mitochondrial damage and biogenesis[J]. Cardiovasc Res, 2004, 64: 279-288.

LORENZON S, DE GUARRINI S, SMITH V J, et al. Effects of LPS injection on circulating haemocytes in crustaceans in vivo[J]. Fish Shellfish Immunol, 1999, 9: 31-50.

LORENZON S, PASQUAL P, FERRERO E A. Different bacterial lipopolysaccharides as toxicants and stressors in the shrimp Palaemon elegans[J]. Fish Shellfish Immunol, 2002, 13: 27-45.

SHIAU S Y,JIANG L C. Dietary zinc requirements of grass shrimp, Penaeus monodon, and effects on immune responses[J]. Aquaculture, 2006, 254: 476-482.

HSU S W, CHEN J C. The immune response of white shrimp Penaeus vannamei and its susceptibility to Vibrio alginolyticus under sulfide stress[J]. Aquaculture, 2007, 271: 61-69.

VERGHESE B, RADHAKRISHNAN E V, PADHI A. Effect of environmental parameters on immune response of the Indian spiny lobster, Panulirus homarus (Linnaeus, 1758) [J]. Fish Shellfish Immunol,2007, 23: 928-936.

TAKAHASHL Y, KONDO M, ITAMI T, et al. Enhancement of disease resistance against penaeid acute viraemia and induction of virus-inactivating activity in haemolymph of kuruma shrimp, Penaeus japonicus, by oral administration of Pantoea agglomerans lipopolysaccharide (LPS) [J]. Fish Shellfish Immunol, 2000, 10: 555-558.

CHANG C F, SU M S, CHEN H Y, et al. Dietary β-1,3-glucan effectively improves immunity and survival of Penaeus monodon challenged with white spot syndrome virus[J]. Fish Shellfish Immunol, 2003, 15: 297-310.

刘立鹤,郑石轩,谭斌,等.不同饲料配方下β-葡囊糖对凡纳滨对虾生长性能、非特异性免疫力的影响及成本分析[J].饲料工业, 2005, 26(8): 26-30.

杨福冈,周洪琪,黄旭雄.不同β-葡聚糖对凡纳滨对虾稚虾生长及非特异免疫功能的影响[J].上海水产大学学报, 2005, 14(3): 263-269.

WANG Y C, CHANG P S, CHEN H Y. Differential time-series expression of immune-related genes of Pacific white shrimp Litopenaeus vannamei in response to dietary inclusion of β-1,3-glucan[J]. Fish Shellfish Immunol, 2008, 24: 113-121.

YOSHIDA T, KRUGER R, INGLIS V. Augmentation of non-specific protection in African catfish, Clarias gariepinus (Burchell), by the long-term oral administration of immunostimulants[J]. J Fish Dis, 1995, 18: 195-198.

CHANG C F, CHEN H Y, SU M S. Immunomodulation by dietary β-1,3-glucan in the brooders of black tiger shrimp, Penaeus monodon[J]. Fish Shellfish Immunol, 2000,10: 505-514.

施引文献

资源附件(0)

访问统计