南海赤潮亚历山大藻的分子鉴定及"塔玛复合种"的分子系统发生分析

刘晨临; 陈艳; 刘胜浩; 黄晓航

南海赤潮亚历山大藻的分子鉴定及"塔玛复合种"的分子系统发生分析

1.
国家海洋局第一海洋研究所海洋生态研究中心, 山东青岛 266061
2.
哈尔滨工业大学研究生院, 黑龙江哈尔滨 150001

基金项目: 国家海洋局青年基金项目(2008110)。

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出版历程
- 收稿日期: 2009-11-19

Molecular identification of red tide Alexandrium isolated from the South China Sea and phylogenetic analysis on "tamarensis complex"

1.
Marine Ecology Centre, Firsrt Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China
2.
graduate school, Harbin Institute of Technology, Harerbin 150001, China

摘要

摘要: 利用SSU rDNA和ITS分子指标,结合GenBank和其他文献中的基因序列构建了系统树,对一株分离自我国南海的亚历山大藻"塔玛复合种"NH01进行了鉴定,发现SSU rDNA 和ITS 序列构建的系统树中,不同地理基因型的"塔玛复合种"均构成独立分支。与我国沿海所有的"塔玛复合种"一致,NH01也属于"亚洲温带"型。探讨了SSU rDNA和ITS分子指标用于塔玛和链状亚历山大藻鉴别的可行性,比较了2种不同的基于ITS序列的亚历山大藻的系统分类方法。认为SSU rDNA和ITS序列均可以应用于亚历山大藻种甚至种下水平的检测,可以准确判断其地理基因型。提出利用SSU rDNA和ITS等核酸序列标记的方法作为鉴定、调研我国海域的亚历山大藻"塔玛复合种"的常规手段。通过比对不同地理基因型的ITS序列,确定了一段DNA序列,可以用于鉴别不同地理基因型的亚历山大藻"塔玛复合种"。
- "塔玛复合种" /
- SSUrDNA /
- ITS /
- 条形码
Abstract: to identify an Alexandrium "tamarensis complex" NH01 isolated from the South China Sea, phylogenetic trees were constructed with SSU rDNA and ITS sequences combined with that from GenBank and literatures. In both SSU rDNA and ITS phylogenetic trees, "tamarensis complex" of different geographical genotypes were subdivided into independent clades. Consistent with other "tarmarensis complexes" isolated from China’s coast area, NH01 also are "temperate Asia" type. The feasibility of identification of "tamarensis complex" with SSU rDNA and ITS sequences was conducted, and two kinds of system classification methods based on ITS sequence were discussed. SSU rDNA and ITS sequences can be used as molecular markers to identification Alexandrium between or even within species, can accurately determine their geographic genotype. Suggesting that a SSU rDNA and ITS nucleotide sequence tag approach is used as a routine method to identify, investigate Alexandrium of China’s coast. Meanwhile, a DNA probe based on ITS sequences was identified and could be used to identify different geographical genotypes of "tamarensis complex".
- "Tamarensis complex" /
- SSU rDNA /
- ITS /
- Barcoding

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参考文献(18)

TAYLOR F J R, FUKUYO Y. The neuorotoxigenic dinoflagellate genus Alexandrium Halim: general introduction[M]//ANDERSON D M, CEMBELLA A D, HALLEGRAEFF G M. Physiological Ecology of Harmful Algal Blooms[M]. Berlin: Springer-Verlag, 1998: 3-12.

陈月琴, 屈良鹄, 曾陇酶, 等. 南海赤潮有毒甲藻链状-塔玛亚历山大藻的分子鉴定[J]. 海洋学报, 1999, 21(3): 106-111.

JOHN U, FENSOME R A, MEDLIN L K. The application of a molecular clock based on molecular sequences and the fossil record to explain biogeographic distributions within the Alexandrium tamarense "species complex"(Dinophyceae) [J]. Mol Biol Evol, 2003, 20(7): 1015-1027.

KI J S, HAN M S. Informative characteristics of 12 divergent domains in complete large subunit rDNA sequences from the harmful Dinoflagellate genus, Alexandrium (Dinophyceae) [J]. J Eukaryot Microbiol, 2007, 54(2): 210-219.

KIM C J, UCHIDA A S, KIM C H. Molecular phyloagenetic relationships whin the genus Alexandrium (Dinophyceae) based on the nuclear-encoded SSU rDNA D1-D2 sequences[J]. J Kor Soc Oceanogr,2004, 39: 172-185.

KIM S H, KIM K Y, KIM C H, et al. Phylogenetic analysis of harmful algal blooming (HAB)-causing dinoflagellates along the Korean coasts based on the SSU rRNA gene[J]. J Microbiol Biotechnol, 2004,14: 959-966.

KI J S, HAN M S. Molecular analysis of complete SSU to LSU rDNA sequence in the harmful dinoflagellate Alexandrium tamarense (Korean isolate, HY970328M) [J]. Ocean Sci J, 2005, 40:155-166.

ADACHI M, SAKO Y, ISHIDA Y, et al. Analysis of Alexandrium (Dinophyceae) species using sequences of the 5.8S ribosomal DNA and internal transcribed spacer regions [J]. J Phycol, 1996, 32: 424-432.

SCHOLIN C A, HALLEGRAEFF G M, ADERSON D M, et al. Molecular evolution of the Alexandrium tamarense "species complex" (Dinophyceae): dispersal in the North American and West Pacific regions[J]. Phycologia, 1995, 34(6): 472-485.

RUIZ S R C, ETHERIDGE S M, COOK P A, et al. Phylogenetic analysis of toxic Alexandrium (Dinophyceae) isolates from South Africa: implications for the global phylogeography of the Alexandrium tamarense species complex[J]. Phycologia, 2005, 44: 49-60.

PENNA A, GARC E, VILA M, et al. Alexandrium catenella (Dinophyceae), a toxic ribotype expanding in the NW Mediterranean Sea[J]. Marine Biology, 2005, 148: 13-23.

黄健, 梁姗, 秦洁. 利用ITS25.8S rDNA 序列对2 株海洋亚历山大藻进行的分子鉴定[J]. 中国海洋大学学报,2008, 38(2): 285-290.

唐祥海, 于仁成, 颜天, 等. 中国沿海亚历山大藻(Alexandrium)核糖体rDNA部分序列分析及该藻属分子系统进化研究[J]. 海洋与湖沼,2006, 11(6): 529-535.

LIU C L, HUANG X H, WANG X L, et al. Phylogenetic studies on two strains of antarctic ice algae based on morphological and molecular characteristics [J]. Phycologia. 2006,45(2): 190-198.

KUMAR S, TAMURE K, NEI M. MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment[J]. Briefings in Bioinformatics. 2004, 5:150-163.

MARIN I, AGUILERA A, REGUERA B, et al. Preparation of DNA suitable for PCR amplification from fresh or fixed single dinoflagellate cells. BioTechniques. 2001, 30 (1): 88-90.

PENNA A, MAGNANI M. Identification of Alexandrium (Dinophyceae) species using PCR and rDNA-targeted probes[J]. J Phycol, 1999, 35: 615-621.

GOTTSCHLING M, KEUPP H, PLOTNER J, et al. Phylogeny of calcareous dinoflagellates as inferred from ITS and ribosomal sequence data[J]. Mol Phylogene Evol 2005, 36(3): 444-455.

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