一株分离自胶州湾的裸甲藻形态相似种的分子系统学研究

朱霞; 甄毓; 于志刚

一株分离自胶州湾的裸甲藻形态相似种的分子系统学研究

1.
中国海洋大学海洋生命学院, 山东青岛 266003
2.
中国海洋大学环境科学与工程学院海洋环境与生态教育部重点实验室, 山东青岛 266100
3.
中国海洋大学化学化工学院海洋化学理论与工程技术教育部重点实验室, 山东青岛 266100

基金项目: 国家自然科学基金项目(40706044);青岛市科技计划基础研究项目(09-1-3-14-jch)。

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出版历程
- 收稿日期: 2010-02-05

Molecular phylogenetic analysis of a Gymnodinium-like species isolated from the Jiaozhou Bay of Shandong Province, China

1.
College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
2.
Key Laboratory of Ministry of Education for Marine Environment and Ecology, College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100 China
3.
Key Laboratory of Ministry of Education for Marine Chemistry Theory and Technology, College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China

摘要

摘要: 对一株分离自胶州湾的裸甲藻形态相似种(Gymnodinium sp. ZX)进行了分子水平的分类鉴定。提取基因组DNA后扩增核糖体小亚基和转录间隔区序列,经纯化、克隆并测序。将获得的序列分别进行Blastn同源性分析,并下载相关序列构建系统进化树,结果表明,该藻与共生藻(Symbiodinium)亲缘关系较近,而与裸甲藻(Gymnodinium)等其他甲藻亲缘关系较远,因此初步鉴定该藻为一种共生藻。用核糖体小亚基和转录间隔区序列分别对共生藻属构建系统进化树,分析各类群之间的关系,初步鉴定本藻属于共生藻属E类群。该藻分离自胶州湾,在f/2培养液中生长良好,表明胶州湾可能存在营自由生活的共生藻。
- 核糖体DNA(rDNA) /
- 转录间隔区 /
- 裸甲藻 /
- 共生藻
Abstract: A strain of microalgae that was isolated from the Jiaozhou Bay and primarily identified as Gymnodinium-like based on the morphological features (named as Gymnodinium sp. ZX) was further distinguished with molecular phylogenetic methods. After the processes of DNA extraction, amplification and purification, the nuclear-encoded small subunit ribosomal DNA (SSU rDNA) and internal transcribed spacer (ITS) regions were cloned and sequenced. Sequences homologous to SSU rDNA and ITS regions of Gymnodinium sp. ZX were searched with Blastn from the GenBank. The phylogenetic trees were reconstructed with the neighbor-joining (NJ) method. The results show that Gymnodinium sp. ZX has a close genetic relationship with Symbiodinium rather than Gymnodinium. Therefore, Gymnodinium sp. ZX was judged as a strain of Symbiodinium sp. Then the phylogenetic trees were reconstructed based on the SSU rDNA and ITS sequences of genus Symbiodinium respectively, the relationship among the various clades was analyzed, and Gymnodinium sp. ZX belonged to clade E. The Gymnodinium sp. ZX was isolated from the Jiaozhou Bay, and grew well in the f/2 medium, therefore, it was inferred that the free-living species of genus Symbiodinium probably existed in the Jiaozhou Bay.
- rDNA /
- ITS /
- Gymnodinium /
- Symbiodinium

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参考文献(34)

周名江, 朱明远, 张经. 中国赤潮的发生趋势和研究进展[J]. 生命科学, 2001, 13(2): 53-59.

王朝晖, 陈菊芳, 徐宁,等. 大亚湾裸甲藻种群的季节变化与环境条件的关系[J]. 生态学报, 2001, 21(11): 1826-1832.

陈国福, 王广策, 张春云, 等. 一株裸甲藻类似种的形态和系统进化分析[J]. 科学通报, 2008, 53(3): 299-305.

庄丽, 陈月琴, 李钦亮, 等. 赤潮叉角藻18S rDNA和ITS区序列测定与分析[J]. 海洋与湖沼, 2001, 32(2): 148-154.

TOMAS C R. Identifying Marine Phytoplankton[M]. San Diego: Harcourt Brace&Company, Academic Press, 1997: 446-447.

SCOTT R, SANTO S, DEREK J, et al . Molecular phylogeny of symbiotic dinoflagellates inferred from partial chloroplast large subunit (23S)-rDNA sequences[J]. Mol Phylogen Evol, 2002, 23(2): 97-111.

GUILLARD R R L, RYTHER J H. Studies on marine planktonic diatoms:Ⅰ. Cyclotellanana Hustedt and Detonula confervacea(Cleve)[J]. Gran Can J Microbiol, 1962, 8: 229-239.

苟万里, 孙军, 何闪英, 等. 几种角毛藻亲缘关系的分子生物学分析[J]. 高技术通讯, 2003, 13(增刊): 68-73.

甄毓. 双特异分子探针技术的建立及对十二种常见赤潮藻的检测 . 青岛: 中国海洋大学, 2006.

KIMURA M. A simple method for estimating evolutionary of base substion through comparative studies of nucleotide sequences[J]. Mol Evol, 1980, 16: 111-120.

SAITOU N, NEI M. The neighbor-joining method: a new method for reconstructing phylogenetic trees[J]. Mol Biol Evol, 1987, 4: 406-425.

FELSENSTEIN J. Confidence limits on phylogenies: an approach using the bootstrap[J]. Evolution, 1985, 39: 783-791.

甄毓, 于志刚, 米铁柱. 分子生物学在微藻分类研究中的应用[J]. 中国海洋大学学报, 2006, 36(6): 875-878.

苟万里, 刘东艳, 甄毓, 等. 利用rDNA和ITS序列对1株裸甲藻的初步鉴定[J]. 中国海洋大学学报, 2003, (34): 75-83.

SALDARRIAGA J F, TAYLOR F J, KEELING P J, et al. Dinoflagellate nuclear SSU rRNA phylogeny suggests multiple plastid losses and replacements[J]. J Mol Evol, 2001, 53(3): 204-213.

LAJEUNESSE T C , TRENCH R K. Biogeography of two species of Symbiodinium (Freudenthal) inhabiting the intertidal sea anemone Anthopleura elegantissima (Brandt)[J]. Biol Bull, 2000, 199(2): 126-134.

SADLER L A, MCNALLY K L, GOVIND N S, et al. The nucleotide sequence of the small subunit ribosomal RNA gene from Symbiodinium pilosum, a symbiotic dinoflagellate[J]. Curr Genet, 1992, 21(4-5): 409-416.

ROWAN R, POWERS D A. Ribosomal RNA sequences and the diversity of symbiotic dinoflagellates (zooxanthellae)[J]. Proc Natl Acad Sci USA, 1992, 89(8): 3639-3643.

CARLOS A A, BAILLIE B K, KAWACHI M. Phylogenetic position of Symbiodinium (Dinophyceae) isolates from Tridacnids (Bivalvia), Cardiids (Bivalvia), a sponge (Porifera), a soft coral (Anthozoa), and a free-living strain[J]. J Phycol, 1999, 35: 1054-1062.

ZHANG H, BHATTACHARYA D, LIN S. A three-gene dinoflagellate phylogeny suggests monophyly of prorocentrales and a basal position for amphidinium and heterocapsa[J]. Mol Evol, 2007, 65(4): 463-474.

THORNHILL D J, LAJEUNESSE T C, SANTOS S R. Measuring rDNA diversity in eukaryotic microbial systems: how intragenomic variation, pseudogenes, and PCR artifacts confound biodiversity estimates[J]. Mol Ecol, 2007, 16(24): 5326-5340.

FREUDENTHAL H D. Symbiodinium gen. nov. and Symbiodinium microadriaticum sp. nov., a zooxanthellae: taxonomy, life cycle, and morphology[J]. J protozool, 1962, 9: 45-52.

POCHON X, MONTOYA-BURGOS J I, STADELMANN B, et al. Molecular phylogeny, evolutionary rates, and divergence timing of the symbiotic dinoflagellate genus Symbiodinium[J]. Mol Phylogen Evol, 2006, 38: 20-30.

朱葆华. 几种腔肠动物共生藻的离体培养及其相关的生理学研究 . 青岛: 中国科学院海洋研究所, 2005.

RAECHEL A L, MADELEINE J H O, WILLIS B L. Methods for sampling free-living Symbiodinium (zooxanthellae) and their distribution and abundance at Lizard Island (Great Barrier Reef)[J]. J Exp Mar Biol Ecol, 2008, 364: 48-53.

MICHAEL S, CARTER D, HOEGH-GULDBERG O. The evolutionary history of Symbiodinium and scleractinian hosts-Symbiosis, diversity, and the effect of climate change[J]. Perspect Plant Ecol Evol Syst, 2006, 8: 23-43.

POCHON X, PAWLOWSKI J, ZANINETTI L, et al. High genetic diversity and relative specificity among Symbiodinium-like endosymbiotic dinoflagellates in soritid foraminiferans[J]. Mar Biol, 2001, 139: 1069-1078.

POCHON X, LAJEUNESSE T C, PAWLOWSKI J. Biogeographic partitioning and host specialization among foraminiferan dinoflagellate symbionts (Symbiodinium, Dinophyta)[J]. Mar Biol, 2004, 146: 17-27.

COFFROTH M A, SANTOS S R. Genetic diversity of symbiotic dinoflagellates in the genus Symbiodinium[J]. Protist, 2005, 156: 19-34.

COFFROTH M A, LEWIS C F, SANTOS S R, et al. Environmental populations of symbiotic dinoflagellates in the genus Symbiodinium can initiate symbioses with reef cnidarians[J]. Curr Biol, 2006, 16: 985-987.

SCOTT R, SANTOS. Comment: phylogenetic analysis of a free-living strain of Symbiodinium isolated from Jiaozhou Bay, P.R. CHINA[J]. J Phycol. 2004, 40: 395-397.

LAJEUNESSE T C. Investigating the biodiversity, ecology, and phylogeny of endosymbiotic dinoflagellates of the genus Symbiodinium using the ITS region: in search of a 'species’ level marker[J]. J Phycol, 2001, 37(5): 866-880.

GRANADOS C, CAMARGO C, ZEA S, et al. Phylogenetic relationships among zooxanthellae (Symbiodinium) associated to excavating sponges (Cliona spp.) reveal an unexpected lineage in the Caribbean[J]. Mol Phylogen Evol, 2008,49: 554-560.

WILCOX T P. Large subunit ribosomal RNA systematics of symbiotic dinoflagellates: morphology does not recapitulate phylogeny[J]. Mol Phylogen Evol, 1998, 10: 436-448.

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