共培养条件下强壮前沟藻与中肋骨条藻的相互作用

冀晓青; 韩笑天; 郑立; 白洁; 俞志明

共培养条件下强壮前沟藻与中肋骨条藻的相互作用

1.
中国科学院海洋研究所海洋生态与环境科学重点实验室, 山东青岛 266071;中国海洋大学环境科学与工程学院, 山东青岛 266100
2.
中国科学院海洋研究所海洋生态与环境科学重点实验室, 山东青岛 266071;国家海洋局第二海洋研究所海洋生态与生物地球化学重点实验室, 浙江杭州310012
3.
国家海洋局第一海洋研究所海洋生态研究中心, 山东青岛 266061
4.
中国海洋大学环境科学与工程学院, 山东青岛 266100
5.
中国科学院海洋研究所海洋生态与环境科学重点实验室, 山东青岛 266071

基金项目: 国家自然科学基金(40806053);国家基础研究规划"九七三"项目(2010CB428700);国家海洋局第二海洋研究所海洋生态系统与生物地球化学重点实验室开放研究基金资助项目(LMEB200701);国家海洋局第一海洋研究所海洋生态环境科学与工程重点实验室开放基金(MESE-2008-02)

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出版历程
- 收稿日期: 2009-11-26

Interactions between Amphidinium carterae Hulburt and Skeletonema costatum(Greville) Cleve with different densities in cultures

1.
Key Laboratory of Marine Ecology and Environmental Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;College of Environment Science and Engineering, Ocean University of China, Qingdao 266100, China
2.
Key Laboratory of Marine Ecology and Environmental Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Key Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration,
3.
Marine Ecology Research Center, First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China
4.
College of Environment Science and Engineering, Ocean University of China, Qingdao 266100, China
5.
Key Laboratory of Marine Ecology and Environmental Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China

摘要

摘要: 半连续条件下研究了强壮前沟藻与中肋骨条藻不同接种密度下种间的相互作用,按照以下条件:(1)1×10⁴ 个/cm³强壮前沟藻与1×10⁴个/cm³中肋骨条藻;(2)1×10⁴个/cm³强壮前沟藻与5×10⁴个/cm³中肋骨条藻;(3)1×10⁴个/cm³强壮前沟藻与10×10⁴个/cm³中肋骨条藻接种进行共培养实验;在五种培养滤液中培养中肋骨条藻,起始密度为1×10⁴个/cm³;利用双藻竞争模型分析两者的竞争抑制参数,结果表明:半连续共培养条件下,强壮前沟藻对三种接种密度的中肋骨条藻都产生强烈的生长抑制作用(P＜0.05),三种接种密度比例下强壮前沟藻对中肋骨条藻的抑制能力分别是中肋骨条藻对强壮前沟藻抑制能力的8.2,3.4和14.2倍;不同接种密度中肋骨条藻对强壮前沟藻的生长抑制作用并没有显著的差异(P＞0.05);中肋骨条藻在五种去藻滤液中生长都受到显著抑制(P＜0.05),强壮前沟藻中肋骨条藻与强壮前沟藻共培养实验结果表明,对中肋骨条藻的生长具有抑制作用,这种抑制作用是可以通过分泌胞外产物来实现,而与细胞的直接接触没有必然联系。
- 强壮前沟藻 /
- 中肋骨条藻 /
- 半连续共培养 /
- 去藻滤液培养 /
- 化感作用
Abstract: In order to investigate the growth interactions between each microalgae, two microalgae Amphidinium carterae Hulburt and Skeletonema costatum(Greville) Cleve were studied with different densities using semi-continuous cultures in controlled laboratory conditions (temperature, light and illuminating periodicity, etc.) and sufficient nutrition supply every day. When 1×10⁴ cell/cm³ A. carterae and 1×10⁴ cells/cm³ S. costatum were inoculated into the same medium, growth of A. carterae was slightly suppressed, while S. costatum was notably suppressed. Other inoculum resulted in reduced S. costatum maximum cell densities. These results were consistent with those of simultaneous assays using cell-free filtrates of mono-algal and semi-continuous cultures. That is, exposure to cell-free filtrates of A. carterae or A. carterae s-S. costatum had a inhibitory effect on S. costatum. A mathematical model was used to simulate growth and interactions of A. carterae and S. costatum in semi-continuous cultures, which indicated that A. carterae outcompeted S. costatum over time. And the inhibition exerted by A. carterae on S. costatum was about 8.2, 3.4 and 14.2 times stronger than the inhibition exerted by S. costatum on A. carterae when they were set at A. carterae and 1×10⁴ cells/cm³ S. costatum, A. carterae and 5×10⁴ cells/cm³ S. costatum and A. carterae and 10×10⁴ cells/cm³ S. costatum, respectively. These results successfully demonstrate that allelopathy is involved in the growth interactions between A. carterae and S. costatum, and extracellular metabolites have been suggested to be one factor contributing to the formation and/or maintenance of dinoflagellate blooms.Cell-free filtrate cultures is one of the basic approaches on allelopathy research which noted that cell-to-cell contact is not necessary for inhibitory effects between algae.
- Amphidinium carterae Hulburt /
- Skeletonema costatum(Greville) Cleve /
- semi-continuous culture /
- cell-free filtrate culture /
- allelopathy

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