海洋硅藻硅质细胞壁结构的形成机理研究概述

梁君荣; 陈丹丹; 高亚辉; 程金凤

海洋硅藻硅质细胞壁结构的形成机理研究概述

1.
厦门大学生命科学学院滨海湿地生态系统教育部重点实验室, 福建厦门 361005
2.
厦门大学生命科学学院滨海湿地生态系统教育部重点实验室, 福建厦门 361005; 西北农林科技大学生命科学学院, 陕西杨凌 712100

基金项目: 国家自然科学基金资助项目(40676082; 40831160519);国家"九七三"项目(2005CB422305)。

计量
- 文章访问数: 2230
- HTML全文浏览量: 20
- PDF下载量: 2338
- 被引次数: 0
出版历程
- 收稿日期: 2009-11-19

A review on the process and mechanism of marine diatom silica structure formation

1.
Key Laboratory of Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361005, China
2.
Key Laboratory of Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361005, China; Northwest Agricultural and Forest University of Science and Technology, Yangling 712100, China

摘要

摘要: 硅藻具有形态各异、结构复杂、精美绝伦的硅质细胞壁,是海洋中进行生物硅化最主要的生物体。硅质细胞壁的形成同样是一个错综复杂的过程,它涉及硅藻细胞将硅酸从胞外转运到胞内;硅酸在细胞内的转移;在硅沉积囊泡(SDV)中的浓缩沉积;最后合成具种类特异性的细胞壁。重点介绍硅酸转运基因(SITs)的分子特征与作用机制;与生物硅化相关的三种蛋白即硅体蛋白(frustulins)、亲硅蛋白(silaffins)和侧壁蛋白(pleuralins)的结构与功能;硅质结构如何在硅沉积囊泡内最终形成的模式。
- 硅藻 /
- 硅质细胞壁 /
- 硅转运 /
- 生物硅化
Abstract: Diatoms have different morphologies, structure complex and beautiful silica cell wall. They are one of the largest groups of silicifying organisms in the ocean. The genetic control and mechanism of this silica structure formation are a very complicated process, including silica acid transportation across biological membranes to cells, silica acid transformation inside cells, silica polymerization within silica deposition vesicle(SDV), and formation of species-specific cell wall and complex silica structure. An overview of current knowledge about the diatom silica structure formation in recent years was provided. It is focused on the detailed structure and function of silica acid transporters(SITs), three proteins associated with biomineralization-frustulins, silaffins and pleuralins, and the mode of the frustule formation inside SDV.
- diatom /
- silica cell wall(frustule) /
- silicon transport /
- biosilicification(biomineralization)

HTML全文

参考文献(56)

NELSON D M, TRGUER P, BREZZINSKI M A, et al. Production and dissolution of biogenic silica in the ocean: revised global estimates, comparison with regional data and relationship to biogenic sedimentation[J]. Global Biogeochem Cycl, 1995, 9: 359—431.

FIELD C B, BEHRENFELD M J, RABERSON J T, et al. Primary production of the biosphere: integrating terrestrial and oceanic components[J]. Science, 1998, 281(5374): 237—240.

KRGER N, SUMPER M. The molecular basis of diatom biosilica formation[M]// BAEUERLEIN E. Biomineralization: Progress in Biology,Molecular Biology and Application. Berlin: Wiley-VCH,2004:137.

HILDEBRAND M, VOLCANI B E, GASSMANN W, et al. A gene family of silicon transporters[J]. Nature, 1997, 385(6618): 688—689.

SUMPER M, BRUNNER E. Silica biomineralization in diatoms: the model organism Thalassiosira pseudonana[J]. Chem Bio Chem, 2008, 9: 1187—1194.

SUMPER M, KRGER N. Silica formation in diatoms: the function of long-chain polyamines and silaffins[J]. Journal of Materials Chemistry, 2004, 14: 2059—2065.

GORDON R, LOSIC D, TIFFANY M A, et al. The glass menagerie: diatoms for novel applications in nanotechnology[J]. Trends in Biotechnology, 2008, 27(2): 116—127.

HAZELAAR S, AVN DER STRATE H J, GIESKES W W C, et al. Monitoring rapid valve formation in the pennate diatom Navicula salinarum(Bacillariophyceae)[J]. Journal of Phycology, 2005, 41(2): 354—358.

GORDON R, DRUM R W. The chemical basis for diatom morphogenesis[J]. Int Rev Cytol 1994, 150: 243—372.

LENOCI L, CAMP P J. Diatom structures templated by phase-separated fluids[J]. Langmuir, 2008, 24: 217—223.

TIFFANY M A. Valve development in Aulacodiscus[J]. Diatom research, 2008, 23: 185-212.

BHATTACHARYYA P, VOLCANI B E. Sodium-dependent silicate transport in the apochlorotic marine diatom Nitzschia alba[J]. Proc Natl Acad Sci U S A, 1980, 77(10): 6386—6390.

HILDEBRAND M. Silicic acid transport and its control during cell wall silicification in diatoms[M]//BAEUERLEIN E. Biomineralization: Progress in Biology, Molecular Biology and Application. Berlin:Wiley-VCH, 2004: 159.

THAMATRAKOLN K, ALVERSON A J, HILDEBRAND M. Comparative sequence analysis of diatom silicon transporters: toward a mechanistic model of silicon transport[J]. Journal of Phycology, 2006, 42(4): 822—834.

GRACHEV MA, DENIKINA N N, BELIKOV S I, et al. Elements of the active center of silicic acid transporters in diatoms[J]. Mol Biol: Mosk, 2002, 36(4): 679—681.

LIKHOSHWAY Y V, MASYUKOVA Y A, SHERBAKOVA T A, et al. Detection of the gene responsible for silicic acid transport in chrysophycean algae[J]. Dokl Biol Sci, 2006, 408: 256—260.

SHERBAKOVA T A, MASYUKOVA Y, SAFONOVA T P, et al. Conserved motif CMLD in silicic acid transport proteins of diatoms[J]. Molecular Biology, 2005, 39: 269—280.

HILDEBRAND M, DAHLIN K, VOLCANI B E. Characterization of a silicon transporter gene family in Cylindrotheca fusiformis: sequences, expression analysis, and identification of homologs in other diatoms[J]. Molecular and General Genetics, 1998, 260(5): 480—486.

SCHRDER H C, PEROVIC-OTTSTADT S, ROTHENBERGER M, et al. Silica transport in the demosponge Suberites domuncula: fluorescence emission analysis using the PDMPO probe and cloning of a potential transporter[J]. Biochemical Journal, 2004, 381: 665—673.

MA J F, TAMAI K, YAMAJI N, et al. A silicon transporter in rice[J]. Nature, 2006, 440(7084): 688—691.

RUETER J G, MOREL F M M. The interaction between zinc deficiency and copper toxicity as it affects the silicic acid uptake mechanisms in Thalassiosira pseudonana[J]. Limnology and Oceanography, 1981, 26(1): 67—73.

GRACHEV M, SHERBAKOVA T, MASYUKOVA Y, et al. A potential zinc-binding motif in silicic acid transport proteins of diatoms[J]. Diatom Research, 2005, 20(2): 409—411.

THAMATRAKOLN K, HILDEBRAND M. Analysis of Thalassiosira pseudonana silicon transporters indicates distinct regulatory levels and transport activity through the cell cycle[J]. Eukaryotic Cell, 2007, 6(2): 271—279.

THAMATRAKOLN K, HILDERBAND M. Silicon uptake in diatoms revisited: a model for saturable and nonsaturable uptake kinetics and the role of silicon transporters[J]. Plant Physiology, 2008, 146(3): 1397—1407.

HILDEBRAND M, DOKTYCZ M J, ALLISON D P. Application of AFM in understanding biomineral formation in diatoms[J]. Pflugers Archiv-European Journal of Physiology, 2008, 456(1): 127—137.

ALVERSON A J. Strong purifying selection in the silicon transporters of marine and freshwater diatoms[J]. Limnology and Oceanography, 2007, 52(4): 1420—1429.

SULLIVAN C W. Diatom mineralization of silicic acid: II. Regulation of silicic acid transport rates during the cell cycle of Navicula pelliculosa[J]. Journal of Phycology 1977, 13(1): 86—91.

HILDEBRAND M, FRIGERI L G, DAVIS A K. Synchronized growth of Thalassiosira pseudonana(Bacillariophyceae) provides novel insights into cell-wall synthesis processes in relation to the cell cycle[J]. Journal of Phycology, 2007, 43(4): 730—740.

POULSEN N, CHESLEY P M, KROGER N. Molecular genetic manipulation of the diatom Thalassiosira pseudonana(Bacillariophyceae)[J]. Journal of Phycology, 2006, 42(5): 1059—1065.

POULSEN N, KRGER N. A new molecular tool for transgenic diatoms: control of mRNA and protein biosynthesis by an inducible promoter-terminator cassette[J]. FEBS J, 2005, 272(13): 3413—3423.

FORSBERG H, LJUNGDAHL P O. Sensors of extracellular nutrients in Saccharomyces cerevisiae[J]. Curr Genet, 2001, 40(2): 91—109.

VAN BELLE D, ANDRE B. A genomic view of yeast membrane transporters[J]. Curr Opin Cell Biol, 2001, 13(4): 389—398.

KRGER N, BERGSDORF C, SUMPER M. A new calcium binding glycoprotein family constitutes a major diatom cell wall component.[J]. EMBO J, 1994, 13(19): 4676—4683.

KRGER N, BERGSDORF C, SUMPER M. Frustulins: domain conservation in a protein family associated with diatom cell walls[J]. Eur J Biochem, 1996, 239(2): 259—264.

KRGER N, DEUTZMANN R, BERGSDORF C, et al. Species-specific polyamines from diatoms control silica morphology[J]. Proc Natl Acad Sci U S A, 2000, 97(26): 14133—14138.

KRGER N, DEUTZMANN R, SUMPER M. Polycationic peptides from diatom biosilica that direct silica nanosphere formation[J]. Science, 1999, 286(5442): 1129—1132.

KRGER N, LEHMANN G, RACHEL R, et al. Characterization of a 200-kDa diatom protein that is specifically associated with a silica-based substructure of the cell wall[J]. Eur J Biochem, 1997, 250(1): 99—105.

VAN DE POLL WH, VRIELING E G, GIESKES W W C. Location and expression of frustulins in the pennate diatoms Cylindrotheca fusiformis, Navicula pelliculosa, and Navicula salinarum(Bacillariophyceae)[J]. Journal of Phycology, 1999, 35: 1044—1053.

KRGER N, WETHERBEE R. Pleuralins are involved in theca differentiation in the diatom Cylindrotheca fusiformis[J]. Protist, 2000, 151(3): 263—273.

KRGER N, LORENZ S, BRUNNER E, et al. Self-assembly of highly phosphorylated silaffins and their function in biosilica morphogenesis[J]. Science, 2002, 298(5593): 584—586.

POULSEN N, SSMPER M, KRGER N. Biosilica formation in diatoms: characterization of native silaffin-2 and its role in silica morphogenesis[J]. Proc Natl Acad Sci USA, 2003, 100(21): 12075—12080.

WONG PO FOO C, HUANG J, KAPLAN D L. Lessons from seashells: silica mineralization via protein templating[J]. Trends Biotechnol, 2004, 22: 577—585.

POULSEN N, KRGER N. Silica morphogenesis by alternative processing of silaffins in the diatom Thalassiosira pseudonana[J]. J Biol Chem, 2004, 279(41): 42993—42999.

ARMBRUST E V, BERGES J A, BOWLER C, et al. The genome of the diatom Thalassiosira pseudonana: ecology, evolution, and metabolism[J]. Science, 2004, 306(5693): 79—86.

PICKETT-HEAPS J D, JEREMY S, ANNA M M, et al. The cell biology of diatom valve formation[J]. Progress in Phycological Research, 1990, 7: 1—167.

VAN DE MEENE A M L, PICKETT-HEAPS J D. Valve morphogenesis in the centric diatom Proboscia alata sundstrom[J]. Journal of Phycology, 2002, 38: 351—363.

VRIELING E G, GIESHES W W C. Silicon deposition diatoms: control by the pH inside the silicon deposition vesicle[J]. Journal of Phycology, 1999, 35: 548—559.

BRZEZINSKI M A, OLSON R J, CHISHOLM S W. Silicon availability and cell-cycle progression in marine diatoms[J]. Mar Ecol Prog Ser, 1990, 67: 83—96.

TAYLOWR N J. Silica incorporation in the diatom Cosinodiscus granii as affected by light intensity[J]. European Journal of Phycology, 1985, 20(4): 365—374.

EDGAR L A, PICKETT-HEAPS J D. Valve morphogenesis in the pennate diatom Navicula cuspidata[J]. Journal of Phycology, 1984, 20(1): 47—61.

PICKETT-HEAPS J D. Cell division and morphogenesis of the centric diatom Chaetoceros decipiens(Bacillariophyceae): I. Living cells[J]. Journal of Phycology, 1998, 34: 989—994.

BLANK G S, SULLIVAN C W. Diatom mineralization of silicic acid: VI. The effects of microtubule inhibitors on silicic acid metabolism in Navicula saprophila[J]. Journal of Phycology, 1983, 19(1): 39—44.

SCHNEPF E, DEICHGRABER G, DREBES G. Morphogenetic processes in Attpheya decora(Bacillariophyceae, Biddulphiineae)[J]. Plant Systematics and Evalution, 1980, 135: 265—277.

HILDEBRAND M. Diatoms, biomineralization processes, and genomics[J]. Chemical Reviews, 2008, 108(11): 4855—4874.

ROGERSON A, DE FREITAS A S W, MCLNNES A G. Growth rates and ultrastucture of siliceous setae of Chaetoeros gracilis(Bacilicariophyceae)[J]. Journal of Phycology, 1986, 22(1): 56—62.

MOCK T, SAMANTA M P, LVERSON V, et al. Whole-genome expression profiling of the marine diatom Thalassiosira pseudonana identifies genes involved in silicon bioprocesses[J]. PNAS, 2008, 105(5): 1579—1584.

施引文献

资源附件(0)

访问统计