中国沿海六个地理群体短蛸的遗传变异研究

吕振明; 李焕; 吴常文; 樊甄姣; 张建设

中国沿海六个地理群体短蛸的遗传变异研究

浙江海洋学院浙江省海洋养殖装备与工程技术重点实验室,浙江舟山 316000

基金项目: 国家科技支撑计划项目(2007BAD43B00);浙江省高等学校优秀青年教师资助计划项目。

计量
- 文章访问数: 1448
- HTML全文浏览量: 37
- PDF下载量: 1022
- 被引次数: 0
出版历程
- 收稿日期: 2009-04-08

Genetic variation of Octopus ocellatus populations in Chinas coastal waters based on the COI gene analysis

Zhejiang Provincial Key Laboratory of Marine Culture Equipement and Technology, Zhejiang Ocean University, Zhoushan 316000,China

摘要

摘要: 采用线粒体COI基因测序技术分析了我国沿海重要经济头足类短蛸(Octopus ocellatus)6个地理群体的遗传结构及其变异,结果表明我国短蛸资源的遗传多样性相对丰富,在654 bp长度的核苷酸片段中共检测到42个多态位点,多态位点比例达6.42 %,检测的60个个体中共获23个单倍型,单倍型多样性指数为0.200~0.867,平均核苷酸多样性指数为0.000 3~0.009 7,平均核苷酸差异数为0.200 ~ 6.311,高于多数海洋头足类的遗传变异。对6个群体的遗传结构进行检测表明,我国的短蛸群体间除部分群体外均存在显著的遗传分化(P＜0.05),遗传结构基本符合脚踏石模型。聚类分析表明6群体已明显分化为两个类群,一个由大连、青岛、连云港群体组成,另一个由舟山、上海和广州群体组成,两类群间存在20个固定位点核苷酸差异,编码的蛋白质存在2个固定氨基酸残基的差异。两类群的分化系数达0.860 2(P＜0.01),基因流大大小于1;AMOVA检测显示仅有12.72%的遗传差异存在于群体内部,而87.28%的遗传差异存在于群体间。短蛸遗传变异的研究成果可为今后我国短蛸资源更好的开发和管理提供理论基础。
- 地理群体 /
- 短蛸 /
- 遗传变异
Abstract: Studies on genetic variation in marine organisms are an important component of successful and sustainable management of marine fishery resources.Genetic variation of six Octopus ocellatus populations in Chinas coastal waters were investigated using the mitochondrial gene(COI) sequencing method. Results show that ,compared with other cephalopod species, O.ocellatus shows higher genetic diversity in populations. 42 polymorphic sites were detected in 654 bp aligned sequence, which reprented for 6.42% of the total length of sequenced COI gene. 23 hapotypes were detected out of 60 individuals from six populations. The hapotype diversity, mean nucleotide diversity and average number of nucleotide differences reached 0.200~0.867, 0.000 3~0.009 7, 0.200~6.311, respectively. Molecular variance analysis revealed strong genetc sturcture in six O.ocellatus populations(P＜0.05). A pattern of genetic-isolation-by-distance, comforming to a stepping stone model, was detected when plotting gene flow against geographic distance.UPGMA tree constructed shows that six O.ocellatus populations studied can be divided into two lineages. One is consisited of Dalian, Qingdao,Lianyungang plulations. The other is consisted of other three populations. Between them, 20 fixed nucleotide sites and two fixed amino acid sites subtitute were detected along the gene sequence and coded protein, respectively. The Fst between them reached 0.8602 (P＜0.01) and the gene flow between them turned out to be much lower than 1. AMOVA test, which showing 12.72% of the genetic variation lies within populations and 87.28% lies between populations, further suggested the genetic differentiation between these two lineages. These findings in the aspect of genetic variation of O.ocellatus will contribute to better exploitment and refined management of this precious cephalopod resources along the coast of China in future.
- geographycal population /
- Octopus ocellatus /
- genetic variation

HTML全文

参考文献(40)

TUDELA S, GARCA-MARN J L, PLA C. Genetic structure of the European anchovy, Engraulis encrasicolus, in the north-west Mediterranean[J]. J Exp Mar Biol Ecol, 1999, 234: 95—109.

AMARAL A, SEQUEIRA M, MARTNEZ-CEDEIRA J, et al.New insights on population genetic structure of Delphinus delphis from the northeast Atlantic and phylogenetic relationships within the genus inferred from two mitochondrial markers[J].Marine Biology, 2007, 151(5):1967—1976.

LINTAS C, HIRANO J,ARCHER S.Genetic variation of the European eel (Anguilla anguilla)[J].Molecular Marine Biology and Biotechnology, 1998, 7(4): 263—269.

FRANKHAM R.Conservation genetics[J]. Annual Review of Genetics, 1995, 29:305—327.

HAN Z Q, GAO T X, TAKASHI Y, et al. Genetic population structure of Nibea albiflora in Yellow Sea and East China Sea[J].Fisheries Science, 2008, 74: 544—552.

权洁霞, 戴继勋,尚迅. 海洋生物遗传多样性研究现状[J].青岛海洋大学学报, 1999, 29(2):283—288.

PAYNE A G,AGNEW D J, PIERCE G J.Trends and assessment of cephalopod fisheries[J]. Fisheries Research, 2006 78:1—3.

SHAW P W. Polymorphic microsatellite DNA markers for the assessment of genetic diversity and paternity testing in the giant cuttlefish, Sepia apama (Cephalopoda) [J]. Conservation Genetics , 2003, 4: 533—535.

PREZ-LOSADA M, GUERRA A, SANJUAN A. Allozyme electrophoretic technique and phylogenetic relationships in three species of Sepia(Cephalopoda: Sepiidae)[J].Comp Bioc Physiol, 1996,114:11—18.

VITTURI R, RASOTTO M B, FARINELLA-FERRUZZA N. The chromosomes of 16 molluscan species[J]. Boll Zool, 1982, 49: 61—71.

TESKE P R, OOSTHUIZEN A, Papadopoulos I, et al. Phylogeographic structure of Octopus vulgaris in South Africa revisited: identification of a second lineage near Durban Harbour[J]. Mar Biol, 2007, 151: 2119—2122.

SANDS C J, JARMAN S N, JACKSON G D.Genetic differentiation in the squid Moroteuthis ingens inferred from RAPD analysis[J]. Polar Biol, 2003, 26: 166—170.

SHAW P W. Polymorphic microsatellite DNA markers in the veined squid Loligo forbesi [J]. Molecular Ecology, 1997, 6: 297—298.

SINCLAIR W, BRISKEY L, ASPDEN W, et al. Genetic diversity of isolated populations of Nautilus pompilius (Mollusca, Cephalopoda) in the Great Barrier Reef and Coral Sea[J]. Reviews in Fish Biology and Fisheries, 2007, 17: 223—235.

郑小东, 王如才, 王昭萍. 头足类遗传变异研究进展[J]. 水产学报, 2001, 25(1): 84—89.

CARVALHO G R,Thompson A, STONER A L. Genetic diversity and population differentiation of the shortfin squid Illex angentinus in the south-west Atlantic [J].Exp.Mar.Biol.Ecol,1992, 158: 105—121.

KASSAHN K S, DONNELLAN S C, FOWLER A J,et al. Molecular and morphological analyses of the cuttlefish Sepia apama indicates a complex population structure [J]. Marine Biology,2003, 143: 947—962.

YEATMAN J, BENZIE J A.Genetic structure and distribution of Photololigo spp. in Australia [J]. Marine Biology, 1994, 118:79—87.

董正之.中国动物志:软体动物门,头足纲 . 北京:科学出版社, 1988.

高强,王昭萍,王如才,等. 五个短蛸群体等位基因酶的遗传变异[J]. 海洋湖沼通报, 2002, 4: 46—51.

SAMBROOK J, FRITSCH E F, MANIATIS T. Molecular cloning //A Laboratory Manual, 2nd edn.Cold Spring Harbor,NY:Cold Spring Harbor Laboratory Press,1989.

LIN X Z, ZHENG X D, XIAO S.Phylogeny of the cuttlefishes (Mollusca: Cephalopoda) based on mitochondrial COI and 16S rRNA gene sequence data[J]. Acta Oceanologica Sinica, 2004, 23(4): 699—70.

杨金权,胡雪莲,唐文乔,等. 长江口邻近水域刀鲚的线粒体控制区序列变异与遗传多样性[J]. 动物学杂志,2008,43(1):8—15.

KATUGIN O N. Genetic variation in the squid Berryteuthis magister(Breey, 1913) (Oegopsida: Gonatidae)[M]//OKUTANI T, O’DOR R K,KUBODERA T.Recent Advances in Fisheries Biology.Tokyo:Tokai University Press, 1993.

ZHENG X D, WANG R C, WANG X F, et al. Genetic variation in population of the common Chinese cuttlefish Sepiella maindroni (Mollusca: Cephalopoda) using allozymes and mitochondrial DNA sequence analysis [J]. J Shellfish Res, 2001, 20: 1159—1165.

KITAURA J, YAMAMOTO G, NISHIDA M. Genetic variation in populations of the diamond-shaped squid Thysanoteuthis rhombus as examined by mitochondrial DNA sequence analysis [J].Fisheries Science, 1998, 64(4): 538—542.

马凌波,张凤英,乔振国,等.中国东南沿海青蟹线粒体COI 基因部分序列分析[J].水产学报, 2006, 30(4) : 463—468.

IGUCHI A, TAKAI S, UENO M,et al.Comparative analysis on the genetic population structures of the deep-sea whelks Buccinum tsubai and Neptunea constricta in the Sea of Japan [J].Mar Biol, 2007, 151: 31—39.

LIU Y, LIU R L, YE L C, et al. Genetic differentiation between populations of swimming crab Portunus trituberculatus along the coastal waters of the East China Sea[J].Hydrobiologia, 2009, 618:125—137.

ARNAUD S, MONTEFORTE M, GALTIER N,et al.Population structure and genetic variability of pearl oyster Pinctada mazatlanica along Pacific coasts from Mexico to Panama [J]. Conservation Genetics, 2000, 1: 299—307.

KHAMNAMTONG B, KLINBUNGA S, MENASVETA P.Genetic diversity and geographic differentiation of the giant tiger shrimp(Penaeus monodon) in Thailand analyzed by mitochondrial COI sequences [J]. Biochem Genet,2009, 47:42—55.

MILLAR C L, LIBBY W J.Strategies for conserving clinal, ccotypic, anadisjunct population diversity in widespread species[M]//FALD D A, HOLSINGER K E. Genetics and Conservation of Rare Plants. New York: Oxford University Press, 1991:149—170.

PALUMBI S R. Population genetics, demographic connectivity, and the design of marine reserves[J]. Ecological Applications:Supplement, 2003, 13(1): 146—158.

ZHENG X D,YANG J M, LIN X Z,et al. Phylogenetic relationships among the Decabrachia cephalopods inferred from mitochondrial DNA sequences[J]. Journal of Shellfish Research,2004, 23(3): 881—886.

郑芳,吕秀玲,孙红英,等.基于线粒体COI 基因序列探讨长江华溪蟹的遗传分化[J]. 南京师范大学学报:自然科学版,2006,29(2):103—105.

孔晓瑜,喻子午,刘亚军,等.中华绒螯蟹与日本绒螯蟹线粒体COI 基因片段的序列比较研究[J]. 青岛海洋大学学报,2001, 31(6): 861—866.

RIGINOS C, VICTOR B C. Larval spatial distributions and other early life history characteristics predict genetic differentiation in eastern Pacific blennioid fishes[J]. Proc R Soc Lond:B, 2001, 268:1931—1936.

MUSS A, ROBERTSON D R, STEPIEN C A, ea al. Phylogeography of Ophioblennius: the role of ocean currents and geography in reef fish evolution[J].Evolution, 2001, 55: 561—572.

RUZZANTE D E, TAGGART C T, Cook D A. Nuclear DNA basis for shelf-and bank-scale population structure in northwest Atlantic cod (Gadus morhua): Labrador to Georges bank[J]. Molecular Ecology, 1998, 7: 1663—1680.

KNOWLTON1 N, WEIGT L A. New dates and new rates for divergence across the Isthmus of Panama[J].Proc R Soc Lond,1998, 265: 2257—2263.

施引文献

资源附件(0)

访问统计

计量

文章访问数: 1448
HTML全文浏览量: 37
PDF下载量: 1022
被引次数: 0

姓名
邮箱
手机号码
标题
留言内容
验证码

留言板