环介导恒温扩增技术快速检测米氏凯伦藻方法的建立

张凤英; 徐兆礼; 马凌波; 郑俊斌; 缪宇平; 陆亚男

环介导恒温扩增技术快速检测米氏凯伦藻方法的建立

中国水产科学研究院, 东海水产研究所, 上海, 200090;农业部海洋与河口渔业资源及生态重点开放实验室, 上海, 200090

基金项目: 上海市科学技术委员会科研计划项目(062358101);中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院东海水产研究所)资助项目(2007M22)

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出版历程
- 收稿日期: 2009-04-09
- 修回日期: 2009-09-02

Loop-mediated isothermal amplification(LAMP) establishment for detection of Karenia mikimotoi(Dinophyceae)

East China Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Shanghai 200090, China;Key and Open Laboratory of Ministry of Agriculture for Marine and Estuarine Fisheries, Shanghai 200090, China

摘要

摘要: 米氏凯伦藻(Karenia mikimotoi,Hansen)属于裸甲藻目(Gymnodiniales),裸甲藻科(Gymnodi-niaceae),凯伦藻属(Karenia)。它能产生溶血性(hemolytic)毒素和鱼毒(ichthyotoxins),溶解鱼类细胞,破坏鱼鳃组织结构,使鱼类无法正常呼吸而窒息死亡^[1]。近年来,米氏凯伦藻引起的赤潮在世界各海域屡次发生,日本海域,墨西哥湾,新西兰、韩国、苏格兰和澳大利亚海域都有米氏凯伦藻引发赤潮的报道,对渔业造成很大损失^[2]。
- 环介导恒温扩增反应 /
- 米氏凯伦藻 /
- 核糖体DNA /
- 分子鉴定
- loop-mediated isothermal amplification (LAMP) /
- Karenia mikimotoi /
- ribosomal DNA /
- molecular idenfication

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参考文献(21)

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