两种造礁石珊瑚共生藻分子系统分类及其对水温升高的响应研究

董志军; 黄晖; 黄良民; 李元超; 时翔

两种造礁石珊瑚共生藻分子系统分类及其对水温升高的响应研究

1.
中国科学院, 南海海洋研究所海洋生物资源可持续利用重点实验室, 广东广州 510301;中国科学院, 烟台海岸带可持续发展研究所, 山东烟台 264003
2.
中国科学院, 南海海洋研究所海洋生物资源可持续利用重点实验室, 广东广州 510301

基金项目: 中国科学院台站基金支持项目(YTZJJ0502);国家海洋局908专项资助项目(908-ST-01-08-珊瑚礁调查);国家自然科学基金项目(40776085;40576052);国家基金委-广东联合基金项目(U0633007)

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出版历程
- 收稿日期: 2008-07-30
- 修回日期: 2009-01-16

Symbiotic algae in two scleractinian corals: molecular phylogeny and their response to elevated temperatures

1.
Key Laboratory of Marine Bioresourses Sustainable Utilization, South China Sea Institute of Oceanlogy, Chinese Academy of Science, Guangzhou 510301, China;Yantai Institute of Coastal Zone Research for Sustainable Development, Chinese Academy of Sciences, Yantai 264003, China
2.
Key Laboratory of Marine Bioresourses Sustainable Utilization, South China Sea Institute of Oceanlogy, Chinese Academy of Science, Guangzhou 510301, China

摘要

摘要: 珊瑚白化是导致全球珊瑚礁生态系统衰退的最重要原因之一,野外观察结果表明不同种属的造礁石珊瑚对于海水温度升高的耐受性有所差异.选取多孔鹿角珊瑚(Acropora millepora)和丛生盔形珊瑚(Galaxea fascicularis)为研究对象,比较其共生藻在温度升高时的光生理差异.对多孔鹿角珊瑚和丛生盔形珊瑚共生藻的分子系统学研究结果表明:多孔鹿角珊瑚和丛生盔形珊瑚的共生藻属于不同系群,丛生盔形珊瑚共生藻属于D系群,而多孔鹿角珊瑚共生藻属于C1亚系群.当温度升高到30℃时并未对两种造礁石珊瑚共生藻光合系统Ⅱ造成损害,而当温度升高到34℃时两种造礁石珊瑚共生藻的F_v/F_m值急剧下降,其光合系统Ⅱ遭受损害.多孔鹿角珊瑚和丛生盔形珊瑚分别与不同系群的共生藻共生可能是导致其对海水温度升高耐受性不同的主要原因,与C1亚系群共生藻共生的多孔鹿角珊瑚对水温升高敏感,容易白化,而与D系群共生藻共生的丛生盔形珊瑚对水温升高的耐受性强,不易白化.
- 珊瑚白化 /
- 造礁石珊瑚 /
- 虫黄藻 /
- 共生藻
Abstract: Coral bleaching was one of the most important causes of decline of coral reef ecosystem globally.Field investigations on mass bleaching events have showed variation in bleaching susceptibility among different coral species.Comparisons were made for thermal stress suscepbility of symbiotic algae in two scleractinian corals Acropora millepora and Galaxea fascicularis.Present study demonstrated that the symbiotic algae in the two scleract inian corals belonged to two clades of the genus Symbiodinium.The scleractinian coral Galaxea fascicularis harbored Symbiodinium clade D,while the scleractinian coral A cropora millepora harbored Symbiodinium subclade C1.No marked reduction in phot ochemical efficiency(F_v/F_m) of photosystem Ⅱ(PSⅡ) was observed in two clades of the genus Symbiodinium while exposed to 30℃ condition However,when it clined to 34℃ significant reduction in photochemical efficiency(F_v/F_m) of PSⅡ occurred in two clades of the genus Symbiodinium.This research indicated that the differences in as sociation with distinct symbiotic algaes were the major causes behind coral bleaching sensit ivities for the two scleractinian coral A cropora millepora and Galaxea fascicularis.The scleractinian coral A cropora millepora associating with Symbiodinium subclade C1 is more sensitive to coral bleaching compared with coral Galaxea fascicularis associating with Sym biodinium Dunder high thermal stress.
- Coral bleaching /
- scleractinian coral /
- zooxanthellae /
- symbiotic algae

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参考文献(30)

Kinzie Ⅲ R A,Takayama M,Santos S R.The adaptive bleaching hypothesis:Experimental tests of critical assumptions[J].Biol Bull,2001,200:51-58.

朱葆华,王广策,黄勃,等.温度、缺氧、氨氮和硝氮对3种珊瑚白化的影响[J].科学通报,2004,49(17):1743-1748.

李秀保,黄晖,符曲,等.鼻形鹿角珊瑚对不同温度的响应及白化研究[J].热带海洋学报,2006,25(6):58-62.

李淑,余克服,施祺,等.南海北部珊瑚共生虫黄藻密度的种间与空间差异及其对珊瑚礁白化的影响[J].科学通报,2007,52(22):2655-2662.

MARSHALl.P A,BAIRD A H.Bleaching of corals on the Great Barrier Reef:differential susceptibilities among taxa[J].Coral Reefs,2000,19:155-163.

FLOROS C D,SAMWAYS M,ARMSTRONG B.Taxonomic patterns of bleaching within a South African coral assemblage[J].Biodiversity and Conservation,2004,13:1175-1194.

WARNER M E,FITT W K,SCHMIDT G W.Damage to photosystem Ⅱ in symbiotic dinoflagellates:A determinant of coral bleaching[J].Proc Natl Acad Sci USA,1999,96,8007-8012.

SAXBY T,DENNISON W C,HOEGH-GULDBERG O.Photosynthetic responses of the coral Mootipora digitata to cold temperature stress[J].Marine Ecology Progress Series,2003,248:85-97.

BHAGOOLI R,HIDAKA M.Comparison of stress susceptibility of in hospite and isolated zooxanthellae among five coral species[J].J Exp Mar Biol Ecol,2003,291:181-197.

ROWAN R.Thermal adaptation in reef coral symbionts[J].Nature,2004,430:742.

HUANG H,DONG Z J,HUANG L M,et al.Restriction fragment length polymorphism analysis of large subunit rDNA of symbiotic dinoflagellates from scleractinian corals in the Zhubi Coral Reef of the Nansha Islands[J].Journal of Integrative Plant Biology,2006,48:148-152.

CHEN C A,YANG Y W,WEI N V,et al.Symbiont diversity in scleractinian corals from tropical reefs and subtropical non-reef communities in Taiwan[J].Coral Reefs,2005,24:11-22.

REIMER J D,TAKISHITA K,MARUYAMA T.Molecular identification of symbiotic dinoflagellates (Symbiodinium app.) from Palythoa spp.(Anthozoa:Hexacorallia) in Japan[J].Coral Reefs,2006,25:521-527.

THOMPSON J D,GIBSON T J,PLEWNIAK F,et al.The ClustalX windows interface:flexible strategies for multiple sequence alignment aided by quality analysis tools[J].Nucleic Acids Res,1997,25:4876-4882.

HALL T A.BioEdit:a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT[J].Nucl Acid Syrup Ser,1998,41:95-98.

KUMAR S,TAMURA K,NEI M.MEGA:molecular evolutionary genetics analysis[CP/DK] [D].University Park,the Pennsylvania State University,1994.

董志军,黄晖,黄良民,等.福建东山附近海域造礁石珊瑚共生藻的分子系统分类和遗传多样性研究[J].台湾海峡,2008,27(2):135-140.

LaJEUNESSE T C.Investigating the biodiversity,ecology,and phylogeny of endosymbiotic dinoflagellates in the genus Symbiodinium using the ITS region:in search of a 'species' level marker[J].J Phycol,2001,37:866-880.

van OPPEN M J H,PALSTRA F P,PIQUET A M T,et al.Patterns of coral dinoflagellate associations in Acropora:significance of local availability and physiology of Symbiodinium strains and host-symbiont selectivity[J].Proc R Soc Lond B Biol Sci,2001,268:1759-1767.

ROWAN R.Diversity and ecology of zooxanthellae on coral reefs[J].J Phycol,1998,34:407-417.

BAKER A C.Flexibility and specificity of coral-algal symbiosis:diversity,ecology,and biogeography of Symbiodinium[J].Annu Rev Ecol Evol Syst,2003,34:661-689.

BAKER A C,STARGER C J,McCLANAHAN T R,et al.Corals" adaptive response to climate change[J].Nature,2004,430:741.

Toiler W W,Rowan R,Knowlton N.Repopulation of zooxanthellae in the Caribbean corals Montastraea annularis and M.faveolata following experimental and disease associated bleaching[J].Biol Bull,2001,201:360-373.

李晓,冯伟,曾晓春.叶绿素荧光分析技术及应用进展[J].西北植物学报,2006,26(10):2186-2196.

梁英,冯力霞,尹翠玲,等.高温胁迫对三角褐指藻和纤细角毛藻叶绿素荧光动力学的影响[J].中国海洋大学学报,2006.36:427-433.

OKAMOTO M,NOJINA S,FURUSHIMA Y,et al.Evaluation of coral bleaching condition in situ using an underwater pulse amplitude modulated fluorometer[J].Fish Sci,2005,71:847-854.

LITTLE A F,van OPPEN M J H,WILLIS B L.Flexibility in algal endosymbioses shapes growth in reef corals[J].Science,2004,304:1492-1494.

GOULET T L,COOK C B,GOULET D.Effect of short-term exposure to elevated temperatures and light levels on photosynthesis of different host symbiont combinations in the Aiptasia pallida/Symbiodinium symbiosis[J].Limnol Oceanogr,2005,50:1490-1498.

COOK A,LOGAN J,WARD B,et al.Elevated temperatures and bleaching on a high latitude coral reef:the 1988 Bermuda event[J],Coral Reefs,1990,9:45-49.

ROBISON J D,WARNER M E.Differential impacts of photoacclimation and thermal stress on the photohiology of four different phylotypes of Symbiodinium (Pyrrhophyta)[J].J Phycol,2006,42:568-579.

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