西太平洋“暖池”深海沉积物中异化型亚硫酸盐还原酶基因多样性分析

杨宁; 赵晶; 王玉桥; 曾润颖

西太平洋“暖池”深海沉积物中异化型亚硫酸盐还原酶基因多样性分析

1.
国家海洋局, 第三海洋研究所, 海洋生物遗传资源重点实验室, 福建厦门 361005
2.
厦门大学, 海洋与环境学院, 海洋技术与工程系, 福建厦门 361005

基金项目: 中国大洋协会项目(DYXM-115-02-2-04);国家海洋局海洋生物遗传资源重点实验室基金(HY200502)资助

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出版历程
- 收稿日期: 2008-07-30
- 修回日期: 2009-02-10

Diversity of dissimilatory sulfite reductase gene in deep sea sediment of the West Pacific “warm pool”

1.
Key Lab of Marine Biogenetic Resource, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China
2.
Department of Marine Technology and Engineering, College of Oceanography and Environmental Science of Xiamen University, Xiamen 361005, China

摘要

摘要: 提取西太平洋"暖池"区深海沉积物样品总DNA,利用异化型亚硫酸盐还原酶(DSR)和甲基辅酶M还原酶(MCR)基因的特异引物,采用PCR-RFLP方法对沉积物样品中这两类功能基因多样性进行研究.该沉积物中的dsrAB基因分别来源于δ-变形菌中的6个属,其中最多的是脱硫弧菌属和脱硫杆状菌属;mcrA基因均来源于产甲烷古菌,其中主要是甲烷微菌.这些基因在系统发育树上都处于相对独立的分支.此外,还有较多的dsrAB,mcrA基因来源于未知的新属或新种.这些结果表明该海区沉积物中由微生物参与的硫和甲烷循环比较活跃,而且其中可能存在多种新的代谢途径.
- 西太平洋“暖池” /
- 深海沉积物 /
- 异化型亚硫酸盐还原酶
Abstract: The diversities of genes specific for dissimilatory sulfite reductase(DSR) and methyl coenzyme Mreductase MCR in metagenomic DNA of deep sediment of the West Pacific warm pool were studied by PCR RFLP using primers specific for dsrAB and mcr A,respectively.The results showed that the dsrA B in the sample were come from 6 genus of δ-Proteobacteria,mainly Desulfovibrio and Desulf obacter.All of the mcrA in the sample were come from archaeal methanogens,mainly Met hanomicrobia.These genes were tend to clustered within independent clades on phylogenetic tree respectively.Fur thermore,many dsr AB and mcr A genes showed high similarity to those from unknown or new species.These results indicated that the microbial metabolisms,which may include some new metabolic pathway,were active in the Sand CH₄ cycles in deep sediment of "warm pool".
- the West Pacific “warm pool” /
- deep sea sediment /
- dissimilatory sulfite reductase

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