微卫星标记在大菱鲆(<i>Scophthalmus maximus</i> L.)家系系谱印证中的应用研究

于飞; 王伟继; 孔杰; 阮晓红

微卫星标记在大菱鲆(Scophthalmus maximus L.)家系系谱印证中的应用研究

1.
中国水产科学研究院黄海水产研究所,山东青岛,266071;上海海洋大学水产与生命学院,上海,200090
2.
中国水产科学研究院黄海水产研究所,山东青岛,266071
3.
中国水产科学研究院黄海水产研究所,山东青岛,266071;中国海洋大学医药学院,山东青岛,266003

基金项目: 国家高技术研究发展规划(“863”项目)(2006AA10A404);黄海水产研究所基本科研业务专项重点课题(2007-chb-05)

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出版历程
- 收稿日期: 2008-12-05
- 修回日期: 2009-05-28

The application of microsatellite markers for genealogical identification in selective breeding program of turbot(Scophthalmus maximus L.)

1.
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Qingdao 266071, China;College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 200090, China
2.
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Qingdao 266071, China
3.
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Qingdao 266071, China;School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China

摘要

摘要: 用8个微卫星标记对大菱鲆7个人工选育家系进行了系谱鉴别和遗传多样性研究。8个微卫星位点的平均多态信息含量为0.6721,平均杂合度为0.7206。8个微卫星位点在7个家系分析中,共检测到40个等位基因,每个位点的等位基因数在38个之间,每个位点平均有5个等位基因。根据已知亲本及子代基因型,成功的推断出了7个家系中缺失亲本的基因型。在双亲未知的情况下8个微卫星位点累积排除概率是97.07%,已知单亲时累积排除概率达99.63%。用UPG-MA法对7个家系的137个子代个体进行了聚类分析,90.5%同一家系的子代个体能完全聚类到一起,分类结果与系谱来源基本一致。微卫星标记可以为大菱鲆混养家系进行亲权鉴定和系谱构建提供技术支持。
- 微卫星 /
- 大菱鲆 /
- 系谱识别
Abstract: Eight microsatellite markers were used to estimate the kinship and the genetic diversity of seven turbot(Scophthalmus maximus L.) families. The average polymorphic information content(PIC) and the average heterozy gosity were 0.6721 and 0.7606,respectively. To tally forty alleles were detected at eight microsatellite loci in seven full and hal-fsib families of turbot. The number of the alleles at each locus ranged from three to eight. Based on the genotypes of either known parentor none of them and of fspring, all the absent parental genotypes of the seven families were deduced. According to the uniquealleles among the families,each family could be disting uished from the others. Cervus 3.0 analysis showed that the combined exclusion probability was 97.07% if parents were both unknown and the combined exclusion probability was 99.63% when only one of the parental geno type was known. A dendrogram was constructed using UPGMA based on genetic similarity matrix. The UPGAM dendrogram of the 137 individuals indicated that 90.5% of the individuals from each family could befully clustered and the classification results accorded with the source of genealogy. The results showed that microsatellite marker is useful tool for genealogical identification of turbot.
- microsatellites /
- Scophthalmus maximus L. /
- genealogical identification

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