一株深海中等嗜热嗜酸菌的分离及鉴定

漆辉洲; 陈红; 敖敬群; 周洪波; 陈新华

一株深海中等嗜热嗜酸菌的分离及鉴定

1.
中南大学, 资源加工与生物工程学院, 湖南, 长沙, 410083
2.
国家海洋局, 第三海洋研究所, 海洋生物遗传工程重点实验室, 福建, 厦门, 361005

基金项目: 国家海洋公益性行业科研专项(200805032);国际海底区域研发项目(DYXM-115-02-2-07)

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出版历程
- 收稿日期: 2008-04-11
- 修回日期: 2009-08-08

Isolation and identification of a strain of moderate thermophilic and acidophilic bacterium from deep sea

1.
School of Resources Processing and Bioengineering, Central South University, Changsha 410083, China
2.
Key Laboratory of Genetic Engineering of Marine Biology, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China

摘要

摘要: 从太平洋热液区样品中分离纯化到一株中等嗜热嗜酸菌,命名为TPY。文中对该菌株的形态、生理生化特征1、6S rDNA序列以及亚铁和单质硫氧化活性进行了研究。TPY菌株为短杆状,革兰氏阳性菌,大小为(0.3~0.5)μm×(1~3)μm;最适生长温度为50℃,最适生长pH值为1.8;该菌既能利用亚铁盐、单质硫自养生长,也能利用酵母粉、葡萄糖、蛋白胨和甘油等有机物异养生长;TPY菌与Sulfobacillus acidophilus(AB089842)的16S rDNA序列高度相似,其同源性为99%。这些结果表明,TPY菌是一株来自深海的嗜酸硫化芽孢杆菌(Sulfobacillus acidophilus),该菌的成功分离将有助于对太平洋热液区微生物种群结构的全面了解。同时,TPY菌对亚铁和单质硫的良好氧化能力显示出其在生物浸矿中的应用潜力。
- 太平洋热液区 /
- 嗜酸硫化芽孢杆菌 /
- 16SrDNA /
- 生物浸矿
Abstract: A moderate thermophilic acidophic bacterium TPY was isolated from hydrothermal vent of the Pacific Ocean, and the study of its morphological characteristics, physiological characterization and 16S rDNA sequence analyses had been done.The strain is Gram positive, rod-shaped in (0.3~0.5)μm×(1~3)μm.The optimal growth temperature is about 50℃, the optimal pH is 1.8.The TPY strain not only can utilize iron and element sulfur as electron donor, but also utilizey east extracts, glucose, peptone and glycerol.The 16S rDNA sequences analysis indicated that the strain TPY was very similar to Sul fobacillus acidophilus (AB089842).Thus the strain TPY was identified as the species Sulf obacillus acidophilus from deep sea.The successful isolation of the strain TPY is helpful to underst and the microo rganic community of hydrothermal vent of the Pacific Ocean.The ability of this strain to oxidize iron and element sulfur shows that it has a good prospective in bioleaching.
- hydrothermal vent of the Pacific Ocean /
- sulf obacillus acidophilus /
- 16S rDNA /
- bioleaching

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参考文献(14)

RAWLINGS D,DEW D,PLESSIS C.Biomineralizstion of metabcontaining ores and concentrates[J].Trends in Biotechnology,2003,21(1):38-44.

BRIERlEY L.细菌的氧化作用.湿法冶金,1996,60:44-47.

KELLY D,WOOD A.Reclassification of some species of Tiobacillus to newly designated genera Acidithiobacillus gen.nov.,Halothiobacillus gen,nov.and Thermithiobacillus gen.nov.[J].International Journal of Systematic and Evolutionary Microbiology,2000,50:511-516.

邓敬石,阮仁满,温建康.中等嗜热菌浸出硫化矿的研究现状及展望[J].矿产综合利用,2002(4):33-38.

ROHWERDER T,GEHRKE T,KINZLER K,et al.Bioleaching review part A:Progress in bioleaching:fundamentals and mechanisms of bacterial metal sulfide oxidation[J].Applied Microbiology and Biotechnology,2003,63:239-248.

OLSON G,BRIERLEY J,BRIERLEY C.Biohaching review part B:Progress in bioleaching:applications of microbial processes by the minerals industries[J].Applied Microbiology and Biotechnology,2003,63:249257.

NORRIS P,CLARK D,OWEN J,et al.Characteristics of Sulfobacillus acidophilus sp.nov.and other moderately thermophilic mineralsulphide-oxidizing bacteria[J].Microbiology,1996,142:775-783.

JOE S,SUTO K,INOIE C,et al.Isolation and characterization of acidophilic heterotrophic iron-oxidiziog bacterium from enrichment culture obtain from acid mine drainage treatment plant[J].Journal of Bioacience and Bioengineering,2007,104(2):117-123.

OKIBE N,GERICKE M,HALLBERG K,et al.Enumeration and characterization of acidophilic microorganisms isolated from a pilot plant stirred-tank biohaching operation[J].Applied and Environment Microbiology,2003,69(4):1936-1943.

杨宇,万民熙,彭宏,等.一株黄铜矿专属浸出细菌的分离与鉴定[J].中南大学学报(自然科学版),2007,38(4):639-644.

陈红,陈新华,4株不同来源的嗜酸氧化亚铁硫杆菌某些生理特征的比较研究[J].台湾海峡,2007,26(4):562-568.

程海娜,胡岳华,高健.一株嗜酸硫氧化菌的分离[J].生命科学研究,2007,11(2):105-109.

SAITOU N,NEI M.The neighbor-joining method:a new method for reconstructing pbylogenetic tree[J].Molecular Biology and Evolution,1987,4:406-425.

李雅芹,何正国.中度嗜热嗜酸铁氧化细菌对Fe²⁺的氧化[J].有色金属,2003,55(1):37-38.

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