我国南部海域条纹斑竹鲨线粒体DNA控制区遗传多态性研究

陈骁; 杨圣云; 潘聪

我国南部海域条纹斑竹鲨线粒体DNA控制区遗传多态性研究

厦门大学, 海洋与环境学院, 福建, 厦门, 361005

基金项目: 我国近海海洋调查与评价专项(908-01-ST09)

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出版历程
- 收稿日期: 2008-03-30
- 修回日期: 2008-09-26

Genetic polymorphism of mitochondrial control region in Chiloscyllium plagiosum from coastal waters of southern China

College of Oceanography and Environmental Science, Xiamen University, Xiamen 361005, China

摘要

摘要: 摘要:在对我国南部沿海闽东、闽南、粤西、海南、北部湾5个海域的条纹斑竹鲨群体线粒体控制区多态性研究中,获得长度为1 094~1 096 bp的线粒体控制区完整序列。序列比对发现了6个多态性核苷酸位点,定义了8个单倍型。5个群体的单倍型多样度(h=0.542 5~0.744 8)和核苷酸多样度(π=0.000 571~0.000 980)均处于较低水平,说明条纹斑竹鲨的线粒体DNA进化速率较低。分子方差分析(AMOVA)结果显示群体间的遗传差异较小(Fst=0.216 26,P<0.000 1),变异主要发生在群体内部。10 000步Markov链计算的群体间遗传分化概率及单倍型系统地理学分析结果将5个群体分成两个地理种群(台湾海峡种群、南海北部种群)。综合分析表明条纹斑竹鲨的基因流主要在浅海近岸水域扩散,遗传变异程度受地理结构和距离隔离影响。
- 中国南部沿海 /
- 条纹斑竹鲨 /
- 线粒体控制区 /
- 基因流
Abstract: The entire mitochondrial control region sequences (1 094—1 096 nucleotides) were amplified from 5 Chiloscyllium plagiosum populations(Mindong, Minnan, Yuexi, Hainan, Beibuwan) in the coastal waters of southern China and 8 haplotypes were defined by 6 poly morphic sites after sequence alignment.The low levels of gene diversity(h=0.542 5~0.744 8) and nucleotide diversity (π=0.000 571~0.000 980) indicate slow rates of mitochondrial DNA evolution in C.plagiosum.The results of AMOVA reveal the variation occur most within populations and minor among populations(Fst=0.216 26, P < 0.000 1).The 5 populations were classified into two geographic populations (Taiwan straits population, Northern South China Sea population) by exact test of population differentiation and nested clade analyses.The results indicate that the gene flow of C.plagiosum expands in coastal waters and genetic variations are affected by geographic structure and distance isolation.
- coastal waters of southern China /
- Chiloscyllium plagiosum /
- mitochondrial control region /
- gene flow

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