基于ITS序列分析的赤潮异弯藻(<i>Heterosigma akashiwo</i>)分子鉴别

梁斌; 陈斌; 洛昊; 郭皓

基于ITS序列分析的赤潮异弯藻(Heterosigma akashiwo)分子鉴别

梁斌¹,
陈斌²,
洛昊²,
郭皓¹

1.
国家海洋环境监测中心, 辽宁, 大连, 116023
2.
国家海洋环境监测中心, 辽宁, 大连, 116023;大连水产学院生命科学与技术学院, 辽宁, 大连, 116023

基金项目: 国家海洋局近岸海域生态环境重点实验室基金资助项目(200504)

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出版历程
- 收稿日期: 2007-11-09
- 修回日期: 2008-08-04

Molecular identification of Heterosigma akashiwo using ITS sequence

1.
National Marine Envionmental Monitoring Center, State Oceanic Administration, Dalian 116023, China
2.
National Marine Envionmental Monitoring Center, State Oceanic Administration, Dalian 116023, China;Department of Life Science and Technology, Dalian Fishery University, Dalian 116023, China

摘要

摘要: 对五株赤潮异弯藻核糖体转录单元内间隔区(ITS区)进行了PCR扩增和序列测定,并结合GenBank中收录的所有赤潮异弯藻ITS序列信息,比较了它们的遗传距离和相似系数,同时结合GenBank中发布的针胞藻纲其他三个属的代表种ITS序列信息,采用邻接法(neighbour-joining,NJ)和最小进化法(minimum evolution,ME)构建系统发育树。结果表明,五株赤潮异弯藻ITS全序列总长度及碱基组成完全一致,均为538 bp,与GenBank中收录的四株赤潮异弯藻仅有一个碱基不同,遗传距离和相似系数分别为0.002和0.980,而与其他赤潮异弯藻ITS序列完全相同,遗传距离和相似系数分别为0.000和1.000。用两种方法构建的系统发育树结构完全一致,赤潮异弯藻属与海洋卡盾藻属亲缘关系较近,而与另外两属的亲缘关系较远。
- 赤潮异弯藻 /
- ITS /
- 分子鉴定
Abstract: The internal transcribed spacer(ITS)region offive strains which collected from Norway,English Channel,New Zealand,U.S.and Qingdao(China)was amplified with polymerase chain-reaction (PCR)and sequenced.Pairwise distance and percentidentity between ITS sequence of the 5 strains and others retrieved from GenBank were ccalculated.The phylogenetic trees(NJ and ME)were constructed with the ITS sequences which linked to other Raphidophyceae.Results showed that the sequence of 5 strains was identical,which was 538 bp,only one base pair different fromo ther four strains saved in GenBank.Pairwise distance and percent identity are 0.002 and 0.098,respectively.Otherwise completed identical sequences had been found when compared with other strains.The phylogenetic trees constructed by two methods showed totally same results.The Heterosig ma had a close relationship with Chattonella, while was distant fromother two classes.
- Heterosigma akashiwo /
- ITS /
- molecular identification

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