坛紫菜耐高温品系选育及经济性状的初步研究
Preliminary study on selecting the high temperature resistance strains and economic traits of Porphyra haitanensis
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摘要: 将人工杂交选育的坛紫菜新品系Z-26,Z-61,Z-81,Z-17的子3代(F3)与野生型对照组进行遗传性状的比较,旨在筛选耐高温品系。实验结果表明:(1)在高温29℃下培养40 d,人工选育的4个新品系丝状体均能正常生长发育,其中Z-26丝状体生长较快,Z-61在29℃下较易成熟,95%的营养藻丝发育成孢子囊枝,Z-17只有30%形成孢子囊枝;(2)Z-26和Z-61品系的叶状体在30℃下培养10 d,虽然藻体变白,但没有死亡,此时将其移至21℃下恢复培养3~4 d,藻体逐渐恢复为原有的色泽;(3)Z-81和Z-17的叶状体在29℃下培养7~10 d,虽然藻体发白但尚未死亡,移至21℃下培养基本可恢复正常,但在30℃下培养10 d,藻体腐烂不可恢复;(4)野生型对照组不耐高温,藻体在27℃下培养5 d就腐烂死亡,人工选育的4个品系耐受高温的幅度比野生型对照组高1~3℃;(5)4个品系叶状体在26~28℃温度下培养10 d后,其主要的藻胆蛋白和叶绿素a的含量与21℃培养下的含量差异不显著,说明短时间的高温培养并不影响其品质。Abstract: Owing to selecting the strains of high temperature resistance,F3 generation of new strains coming from hybridizationin Porphyra haitanensis(Z-26,Z-61,Z-81,Z-17)was compared with the wild-type control group by inheritable traits.Results indicated that:(1)The four new strains of selective filament strains could normally growand develop after 40 d cultivated at 29℃ high temperature.Such as Z-26 filament grew faster;Z-61 was easily mature and 95% of the conchocelis filament sprouted into sporangial branch.There was only 30% of filament sprouted into sporangial branch in Z-17.(2)Z-26 and Z-61 were cultivated 10 d under the temperature of 30℃.Then they turned white but were not dead and could return theirintrinsical colour after 3~4 d cultivated at 21℃.(3)Blades of Z-81 and Z-17 which were cultivated 7~10 d at 29℃ turned white but were not dead and could return normal at 21℃.However,when cultivated 10 d under the temperature of 30℃,they were rotten and could not be recovered.(4) Wild-type control group was no high temperature resistance and they were rotten even dead after 5 days cultivated under the temperature of 27℃.The four stains of slection could resistthe high temperature, 1~3℃ higher,comparing with the wild-type control group.(5)The thallus of the four strains were cultivatied 10 d under the temperature of 26~28℃ and their main phycobiliprotein and chlorophyll a were quiet variance comparing with the thallus that were cultivated under the temperature of 21℃.It indicated thattemporary high temperature did not make effect on their quality.
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Key words:
- Porphyra haitanensis /
- strains /
- high temperature /
- growth and development
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