RAPD对红鳍笛鲷(♀)×千年笛鲷(♂)子一代杂种优势预测的分析
Prediction of the heterosis in hybrids of red snapper Lutjanus erythropterus(♀)×L.sebae(♂)using RAPD
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摘要: 应用RAPD技术对红鳍笛鲷(♀)、千年笛鲷(♂)和红鳍笛鲷(♀)×千年笛鲷(♂)子一代3个群体的RAPD扩增带谱进行了分析,并对杂交子代F1杂种优势进行了预测。从100个随机引物中筛选出35个扩增带丰富的引物进行扩增,共得到190条清晰稳定的扩增带,其中有176个扩增位点具有多态性,多态性位点比率为92.6%。红鳍笛鲷、F1、千年笛鲷3个群体的遗传相似性指数分别为0.831 9,0.726 3,0.916 8;Nei多样性指数分别为0.174 5,0.264 0,0.118 4;Shannon信息指数分别为0.256 4,0.384 5,0.171 2。红鳍笛鲷(♀)-F1,F1-千年笛鲷(♂),红鳍笛鲷(♀)-千年笛鲷(♂)的遗传距离分别为0.397 2,0.317 0,0.854 1。从相似性指数、Nei多样性指数和Shannon信息指数均显示F1代有杂种优势,并找到了种间特异性标记。Abstract: The heterosis of F1 hy brids(Lutjanus erythropterus ♀ × L.sebae ♂) was predicted with the RAPD method.35 arbitrary primers were screened to amplify the genomic DNA of these three populations of red snapper.190.distinct and highly reproducible RAPD bands with high genetic variability were detected and the frequency of polymorphism was 92.6%.Among the F1 population,L.erythropterus population and L.sebae population,the values of genetic identitiy were 0.726 3,0.831 9,0.916 8,respectively; the values of Nei's gene diversity were 0.264 0,0.174 5,0.118 4,respectively;the values of Shannons informationindex were 0.384 5,0.256 4,0.171 2,respectively,which all indicated that F1 populations showed greater genetic diversity than parental populations.Furthermore,the genetic distance between hybrid populations and L.erythropterus ♀ was 0.397 2,0.317 2 between hybrid populations and L.sebae ♂ and 0.854 1 between L.erythropterus ♀ and L.sebae ♂.The data of S,H and I all showed that F1 owned the potential heterosis.Finally,some species-specific markers were found.
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Key words:
- Lutjanus erythropterus /
- L.sebae /
- hybrid /
- heterosis /
- RAPD
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陈国华,尹绍武,雷从改,等.杂交笛鲷人工繁育技术的初步研究[J].水产科学,2006,25(1):1-4. 夏德全,曹萤,吴婷婷,等.用RAPD分析对罗非鱼遗传变异的研究及其对杂种优势的应用[J].水产学报,1999,23(1):27-32. 吴敏生,戴景瑞.AFLP标记与玉米杂种产量杂种优势的预测[J].植物学报,2000,42(6):600-604. 谢晓东,倪中福,盂凡荣,等.小麦杂交种与亲本发育早期种子的基因表达差异及其与杂种优势关系的初步研究[J].遗传学报,2003,30(3):260-266. 赵文学,杨星,彭智,等:黄颡鱼属物种的RAPD分子鉴定及杂种遗传分析[J].水生生物学报,2006,30(1):101-106. 夏德全,曹萤,杨弘,等.罗非鱼杂交F1代与亲本的遗传关系及其杂种优势的利用[J].中国水产科学,1999,6(4):29-32. 董在杰,夏德全,吴婷婷,等.RAPD技术在鱼类杂种优势研究中的应用[J].中国水产科学,1999,6(1):37-40. 董在杰,夏德全,吴婷婷,等.兴国红鲤和散鳞镜鲤杂种优势的RAPD分析[J].上海水产大学学报,1999,8(1):31-36. 滕丽莉,杨爱国,赵峰,等.栉孔扇贝X虾夷扇贝子一代杂种优势的RAPD分析[J].高技术通讯,2005,15(6):97-101. 萨姆布鲁克.分子克隆实验指南(第三版,2002年)[M].北京:科学出版社,2005. WILLIAMS J G,KUBELIK A R,LIVAK K J,et al.DNA polymorphisms amplified arbitrary primers are useful as genetic markers[J].Nucleic Acids Res,1990,18(22).-6531-6535. NEI M.Mathematical modeling for studying genetic variation in terms of restriction endonucleases[J].Proceedings of the National Academy of Science,1979,76:5268-5273. YEH FoYANG R C,BOYLE T.[CP/OL] [2001-o9] http://www.ualberta,ca/～fyeh/info,htm. 楼允东.鱼类育种学[M].北京:中国农业出版社,1999,53-62. BOPPENMAIER J,MELCHINGER A E,SEITZ G,et al.Genetic diversity for RFLPs in European maize inbreds.Performance of crosses within versus between heterotic groups for grain traits[J].Plant Breed,1993,111:217-226. 杨弘,夏德全,刘莆.等.奥利哑罗非鱼(♀)、鳜(♂)及其子代问遗传关系的研究[J].水产学报,2004,28(5):594-598. 万俊芬,汪小龙,潘洁,等.日本盘鲍X皱纹盘鲍子代杂种优势的RAPD分析[J].青岛海洋大学学报,2001,31(7):506-512. -
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