缢蛏六群体线粒体DNA-COI基因序列变异及群体遗传结构分析

牛东红; 李家乐; 沈和定; 姜志勇

缢蛏六群体线粒体DNA-COI基因序列变异及群体遗传结构分析

上海水产大学省部共建水产种质资源发掘与利用教育部重点实验室, 上海 200090

基金项目: 国家"863"计划项目(2006AA10A410);上海市重点学科建设项目(Y1101)资助

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出版历程
- 收稿日期: 2007-05-11
- 修回日期: 2008-02-22

Sequence variability of mitochondrial DNA-COI gene fragment and population genetic structure of six Sinonovacula constricta populations

Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of Education, Shanghai Fisheries University, Shanghai 200090, China

摘要

摘要: 利用线粒体DNA细胞色素氧化酶亚基(COI)基因序列片段对江浙闵沿海地区缢蛏三个野生群体(江苏射阳-WS、浙江象山-WX,福建霞浦-WP)和三个养殖群体(江苏射阳-CS、浙江象山-CX、福建焦城-CJ)的遗传结构进行初步分析.以特异引物进行PCR扩增,经纯化、测序、同源序列比对获得长度为556bp的核苷酸序列,其中A+T含量为66.2%,显著高于G+C含量.在缢蛏六群体98个个体中共检测到了56个单倍型和66个核苷酸多态位点,多态位点比例为11.7%.野生群体的平均核苷酸差异数和核苷酸多样性指数略高于养殖群体,但是野生群体和养殖群体的单倍型多态性指数均大于0.9,其单倍型较为丰富.此外,六群体均有各自特有的单倍型,但只有射阳野生群体与其他群体无共享单倍型,具有鉴定该群体的特异碱基序列.遗传距离和聚类结果显示,养殖群体与象山野生群体和霞浦野生群体间亲缘关系较近.
- 缢蛏 /
- 线粒体DNA /
- COI /
- 遗传多样性
Abstract: The Razor Clam,Sinonovacula constricta,is distributed along the coastal waters of West pacific ocean which is one of the four most valuable and popular shellfish resources in China.To examine the genetic diver sity in three wild and three cultured S.constricta populations from Jiangsu,Zhejiang and Fujian Provinces,mitochondrial cytochrome oxidase subunit Ⅰ gene(COI) was deter mined.A 556bp long partial genesegment was acquired and sequences were moderately A+Trich(A+T=66.2%),which was higher than G+C content.The results showed that 56 haploty pes and 66 variation sites were found in the sequences of the 98 samples.The nucleotide diversity and average number of differences of wild populations were a few higher than that of cultured populations,but the values of haplotype diversity of six populations were 0.9.Moreover,there were special haplotypes in wild Jiang supopulation,which were not shared with others'.The genetic distance and cluster analysis suggested that the phylogenetic relationship of three cultured populations was closed to the Zhejiang and Fujian wild populations in Zhejiang and Fujian Provinces.
- Sinonovacula constricta /
- mtDNA /
- COI /
- genetic diversity

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参考文献(34)

BIRKY C W,FUERST P,MARRUYAMA T.Organelle gene diversity under migration,mutation and drift:equilibrium expectations,approach to equilibrium,effects of heteroplasmic cells,and comparison to nuclear genes[J].Genetics,1998,121:613-627.

KAPPNER I,BIELER R.Phylogeny of venus clams (Bivalvia:Venerinae) as inferred from nuclear and mitochondrial gene sequences[J].Molecular Phylogenetics and Evolution,2006,40:317-331.

BARRUCCA M,OLMO E,SCHIAPARELLI S,et al.Molecular phylogeny of the family Pectinidae (Mollusca:Bivalvia) based on mitochondrial 16S and 12S rRNA genes[J].Molecular Phylogenetics and Evolution,2004,31:89-95.

KIRSTEN M,DONALD Q,MARTYN K,et al.The phylogeny and taxonomy of austral monodontine topshells (Mollusea:Gastropoda:Trochidae),inferred from DNA sequences[J].Molecular Phylogenetics and Evolution,2005,37:474-483.

陈丽梅,孔晓瑜,喻子牛,等.3种蛏类线粒体16SrRNA和COI基因片段的序列比较及其系统学初步研究[J].海洋科学,2005,29(8):27-32.

张亚平,施立明.动物线粒体DNA多态性的研究概况[J].动物学研究,1992,13(3):289-298.

SHI L,WANG J.Biochemical and immunological characterization of excretorys ecretory products of Vesicocoelium solenophagum and plasma proteins of its bivalve host,Sinonovacula constricts[J].J Helminthol,2001,75 (3):279-284.

雷晓凌,吴红棉,范秀萍,等.缢蛏糖胺聚糖的提取分离及其体外抗肿瘤活性的初步研究[J].药物生物技术,2004,11(3):146-149.

胡虹,金国虔,吴梧桐,等.连云港沿海5种贝类生物的遗传多样性分析[J].药物生物技术,2006,13(6):418-421.

FOLMER O,BLACK M,HOEH W,et al.DNA premiers for amplification of mitochondrial cytochrome eoxidase subunit I from diverse metazoan invertebrate[J].Mol Mar Biol Biotechnol,1994,3:294-299.

孔晓瑜,相建海.魁蚶线粒体16S rRNA和COI基因片段序列测定及其应用前景[J].海洋科学,2001,25(12):46-48.

曲若竹,侯林,吕红丽,等.群体遗传结构中的基因流[J].遗传,2004,26(3):377-382.

田风贵,马志杰,陈智华.分子生态学与生物多样性研究[J].西南民族大学学报(自然科学版),2005,31(1):115-120.

ZBAWICKA M,WENNE R,SK1BINSKI D O F.Mitochondrial DNA variation in populations of the mussel Mytilus trossulus from the Southern Baltic[J].Hydrobiologia,2003,499(1-3):1-12.

李红蕾,宋林生,刘保忠,等.栉孔扇贝不同种群的遗传结构及其杂种优势[J].海洋与湖沼,2002,33(2):188-195.

罗巍,邹丽珍,郑天凌,等.近江牡蛎核糖体DNA片段基因序列及其分子分类研究[J].台湾海峡,2005,24(3):322-330.

WRIGHT S.Evolution in Mendelian population[J].Genetics,1931,16:91-159.

ALARCON J A,MAGOULAS A,GEORGAKOPOULOS T,et al.Genetic comparison of wild and cultivated European populations of the gilthead sea bream (Sparus aurata)[J].Aquaculture,2004,230(1-4):65-80.

黎中宝,林鹏.我国不同纬度白骨壤种群遗传多样性和遗传分化的研究[J].海洋学报,24(1):142-147.

郑向忠,徐宏发,陆厚基.动物种群遗传异质性研究进展[J].生物多样性,1997,5(3):210-216.

WHITOCK MC,DAVIDEM.Indirect measures of gene flow and migration:FST≠1/(4Nm+1)[J].Heredity,1999,82:117-125.

LENORMAND T,GUILI.EMAUD T,BOURGUET D,et al.Evaluating gene flow using selected markers:a case study[J].Genetics,1998,149:1383-1392.

王冬群,李太武,苏秀榕.缢蛏六个种群的生化遗传标记[J].水产科学,2004,23(4):8-11.

季军,杨四海,田大成.水稻基因组中抗病基因正选择方式及基因转换的研究[J].中国农业科学,2007,40(9):1856-1863.

王霞,潘国庆,庞敏,等.家蚕核型多角体病毒bro基因的单核苷多态性研究[J].蚕业科学,2005,31(3):294-299.

李易.基因进化的同义与非同义替代计算及统计检验的比较分析[J].曲靖师范学院学报,2006,25(6):2-9.

HUANG B X,PEAKALL R,HANNA P J.Analysis of geneticstructure of blacklip abalone (Haliotis rubra) populations using RAPD minisatellite and mierosatellite markers[J].Marine Biology,2000,136:2-7.

POWELL W,MORGANTE M,ANDRE C,et al.The comparison of RFLP,RAPD,AFLP and SSR markers for germplasm analysis[J].Mol Breeding,1996,2:2-5.

李太武,孙修勤,刘艳,等.栉孔扇贝种群的遗传变异分析[J].高技术通讯,2001,11(4):25-27.

宋林生,李俊强,李红雷,等.用RAPD技术对我国栉孔扇贝野生种群与养殖种群的遗传结构及其遗传分化的研究[J].高技术通讯,2002,7:83-86.

陈省平,包振民,潘洁,等.4种养殖扇贝的群体遗传多样性及特异性AFLP标记研究[J].海洋学报,2005,27(2);160-164.

李成华,李太武,宋林生,等.4个缢蛏群体遗传结构的RAPD分析[J].水产科学,2004,23(12):26-28.

牛东红,李家乐,汪桂玲,等.缢蛏六群体16SrRNA基因片段序列的差异分析[J].上海水产大学学报,2007,16(1):1-6.

刘亚军,喻子牛,姜艳艳,等.栉孔扇贝16SrRNA基因片段序列的多态性研究[J].海洋与湖沼,2002,33(5):477-483.

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