缢蛏六群体线粒体DNA-COI基因序列变异及群体遗传结构分析

牛东红; 李家乐; 沈和定; 姜志勇

缢蛏六群体线粒体DNA-COI基因序列变异及群体遗传结构分析

上海水产大学省部共建水产种质资源发掘与利用教育部重点实验室, 上海 200090

基金项目: 国家"863"计划项目(2006AA10A410);上海市重点学科建设项目(Y1101)资助

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出版历程
- 收稿日期: 2007-05-11
- 修回日期: 2008-02-22

Sequence variability of mitochondrial DNA-COI gene fragment and population genetic structure of six Sinonovacula constricta populations

Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of Education, Shanghai Fisheries University, Shanghai 200090, China

摘要

摘要: 利用线粒体DNA细胞色素氧化酶亚基(COI)基因序列片段对江浙闵沿海地区缢蛏三个野生群体(江苏射阳-WS、浙江象山-WX,福建霞浦-WP)和三个养殖群体(江苏射阳-CS、浙江象山-CX、福建焦城-CJ)的遗传结构进行初步分析.以特异引物进行PCR扩增,经纯化、测序、同源序列比对获得长度为556bp的核苷酸序列,其中A+T含量为66.2%,显著高于G+C含量.在缢蛏六群体98个个体中共检测到了56个单倍型和66个核苷酸多态位点,多态位点比例为11.7%.野生群体的平均核苷酸差异数和核苷酸多样性指数略高于养殖群体,但是野生群体和养殖群体的单倍型多态性指数均大于0.9,其单倍型较为丰富.此外,六群体均有各自特有的单倍型,但只有射阳野生群体与其他群体无共享单倍型,具有鉴定该群体的特异碱基序列.遗传距离和聚类结果显示,养殖群体与象山野生群体和霞浦野生群体间亲缘关系较近.
- 缢蛏 /
- 线粒体DNA /
- COI /
- 遗传多样性
Abstract: The Razor Clam,Sinonovacula constricta,is distributed along the coastal waters of West pacific ocean which is one of the four most valuable and popular shellfish resources in China.To examine the genetic diver sity in three wild and three cultured S.constricta populations from Jiangsu,Zhejiang and Fujian Provinces,mitochondrial cytochrome oxidase subunit Ⅰ gene(COI) was deter mined.A 556bp long partial genesegment was acquired and sequences were moderately A+Trich(A+T=66.2%),which was higher than G+C content.The results showed that 56 haploty pes and 66 variation sites were found in the sequences of the 98 samples.The nucleotide diversity and average number of differences of wild populations were a few higher than that of cultured populations,but the values of haplotype diversity of six populations were 0.9.Moreover,there were special haplotypes in wild Jiang supopulation,which were not shared with others'.The genetic distance and cluster analysis suggested that the phylogenetic relationship of three cultured populations was closed to the Zhejiang and Fujian wild populations in Zhejiang and Fujian Provinces.
- Sinonovacula constricta /
- mtDNA /
- COI /
- genetic diversity

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