东海三疣梭子蟹第一次卵巢发育规律的研究

吴旭干; 姚桂桂; 杨筱珍; 成永旭; 王春琳

东海三疣梭子蟹第一次卵巢发育规律的研究

1.
上海水产大学, 农业部水产种质资源与养殖生态重点开放实验室, 上海, 200090
2.
宁波大学, 生命科学与生物工程学院, 宁波, 315211

基金项目: 国家863高技术研究发展计划项目(2006AA10-A406);浙江省重大科技攻关项目(No.2004C12029);农业部水产种质资源和养殖生态重点开放实验室开放课题(No.KFI2006-3);上海水产大学青年基金(科05-152);上海市重点学科建设项目(No.Y1101)

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出版历程
- 收稿日期: 2006-12-08
- 修回日期: 2007-02-16

A study on the ovarian development of Portunus trituberculatus in East China Sea during the first reproductive cycle

1.
Key Laboratory of Aquatic Genetic Resources and Aquaculture Ecosystem Certificated by the Ministry of Agriculture, Shanghai Fisheries University, Shanghai 200090, China
2.
College of Life Science and Biotechnology, Ningbo University, Ningbo 315211, China

摘要

摘要: 2004年7月至2005年4月在舟山海区定点连续采样,系统研究了东海三疣梭子蟹卵巢发育期间的卵巢指数(GSI)、肝胰腺指数(HSI)、卵巢外部特征、卵巢发育分期及其组织学的变化.结果表明:东海三疣梭子蟹生殖蜕壳和交配主要发生在9~10月,产卵主要在3~4月,卵巢发育早期不同个体间的GSI差异较大,后期则趋于同步;三疣梭子蟹卵巢发育期间,GSI显著增加,HSI呈现先上升后下降的趋势,GSI和HSI呈负相关性;根据GSI、卵巢形态特征和组织学变化等,三疣梭子蟹卵巢发育可以分为6期,Ⅰ期卵巢呈透明细带状,雌体尚未生殖蜕壳,卵巢中主要为卵原细胞(OG)和卵黄合成前的卵母细胞(PR);Ⅱ期卵巢呈乳白色,主要为卵黄合成前的卵母细胞(PR)和内源性卵黄合成期的卵母细胞(EN);Ⅲ期卵巢为淡黄色或橘黄色,主要为外源性卵黄合成期的卵母细胞(EX);Ⅳ期卵巢呈橘红色,主要为近成熟的卵母细胞(NO),卵径为200~300μm;Ⅴ期卵巢发育成熟,主要为成熟期的卵母细胞(MO);Ⅵ期卵巢呈淡橘红色,已排卵,卵巢中主要为排卵后剩余的基膜和滤泡核.
- 三疣梭子蟹 /
- 卵巢发育 /
- 卵巢指数 /
- 卵母细胞
Abstract: By the continuous sampling in Zhoushan sea area from July 2004 to April 2005,the study as conducted to invest igate the change of gonadosomatic index(GSI),hepatosomatic index(HSI),external features of ovary(size,morphology,color,etc),ovarian histology and its stages during the first reproductive cycle of Portunus trituberculatus.The results indicated that,the reproductive molt of Portunus trituber culatus occurs from September to October,while it spawns from March to April.During the ovarian development,the GSI increases significantly,while the HSI rises slightly,then declines gradually.The negative correlation could be found between GSI and HSI during the period.Based on GSI,HSI,external features of ovary,and histological configuration,the process of ovarian development could be divided into six stages,Ⅰ the ovary is ribbonlike and transparent,the main cells in the ovary are oo gonia(oo)and previt ellogenic oocyte(PR)before reproductive molt;Ⅱ the ovary is milk white,the main cells in the ovary are endogenous vitellogeic oocyte(EN)and PR;Ⅲ the ovary is buff and orange,the main cell type in the ovary is exogenous vitellogenic oocyte(EX);Ⅳ the ovary is deep orange,the main cell types are EX and nearly-mature oocyte(NO)in the ovary;Ⅴ the ovary is ripe,the main cell type is mature oocy te(MO); Ⅵ the crab has spawned,the ovary is light orange,the basal laminas(BL)and follicular cells(FC)could be found in the ovary.
- Portunus trituberculatus /
- ovarian development /
- gonadosomatic index(GSI) /
- oocyte

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