8种抗生素对塔玛亚历山大藻生长的影响

张冬宝; 隋正红; 茅云翔; 郭浩; 臧晓南; 张学成; 吕英刚

8种抗生素对塔玛亚历山大藻生长的影响

1.
中国海洋大学生命科学与技术学部海洋生物遗传与种质工程实验室, 山东青岛 266003
2.
国家海洋局海洋环境监测中心, 辽宁大连 116023
3.
青岛国风药业股份有限公司, 山东青岛 266510

基金项目: 瑞典国防科学基金课题资助(A/3864-1)

计量
- 文章访问数: 1211
- HTML全文浏览量: 111
- PDF下载量: 1416
- 被引次数: 0
出版历程
- 收稿日期: 2005-08-05
- 修回日期: 2006-03-20

Study on the sensitivities of eight antibiotics on Alexandrium tamarense (Lebour) Balech

1.
Laborotary of Marine Genetics and Breeding, Division of Life Science and Technology, Ocean University of China, Qingdao 266003, China
2.
National Marine Environmental Monitoring Center, State Oceanic Administration, Dalian 116023, China
3.
Qingdao Growful Pharmaceutical Corpouation Lat., Qingdao 266510, China

摘要

摘要: 对一株有毒的塔玛亚历山大藻(Alexandrium tamarense(Lebour) Balech)进行了8种抗生素-氯霉素、红霉素、林可霉素、庆大霉素、金霉素、氨苄青霉素、新霉素和链霉素的敏感性实验.结果表明,该藻对抗生素较为敏感.其中的5种:氯霉素、红霉素、林可霉素、庆大霉素和金霉素,对藻8d比生长速率的EC₅₀分别为2.45μg/cm³,25.2μg/cm³,7.95μg/cm³,285u/cm³,58.7μg/cm³,可用作该藻基因工程的选择压力,其对应的抗性基因可以作为基因工程阳性选择标记基因,应用于塔玛亚历山大藻的遗传操作研究.新霉素和链霉素对A.tamarense的生长无明显抑制作用,可以用于藻株无菌化培养而应用于甲藻产毒机制和赤潮爆发机理的研究,还应用筛选出的抗生素对藻培养物进行了除菌实验.
- 抗生素 /
- 基因工程 /
- 塔玛亚历山大藻 /
- 无菌化 /
- 选择标记
Abstract: The sensitivities of a toxic strain of Alexandrium tamarense(Lebour) Balech to eig ht antibiotics:chloram phenicol(Cm),erythromy cin(Er),lincomy cin(Lm),gentamicin(Gm),auromycin(Aum),ampicillin(Amp),neomycin(Nm) and strepto mycin(Str) were reported in the present studies.The result revealed that this dinoflag ellate was sensitive to five of the eight antibiotics.The EC50(50% effective concent ration) of specific growth rate after 8 days were 2.45 Lg/cm³,25.2 Lg/cm³,7.95 Lg/cm³,285 u/cm³ and 58.7 Lg/cm³ for Cm,Er,L m,Gm and Aum respectively.The five antibiotics were applicable as selective reagents,and the correspondent genes maybe employed as positive selective markersing enetic engineering of Alexandrium tamarense.On the contrary,Nm and Strhad noinhibition effects on the growth of the algae.These two antibiotics could be used in sterile purification of A.tamarense for clarifying the mechanism of the toxigenicity and the out break of the red tide.The inhibition effect of the selected antiobiotics were further tested.
- Alexandrium tamarense /
- antibiotics /
- genetic engineering /
- selective marker /
- strain sterilization

HTML全文

参考文献(23)

齐雨藻,邹景忠,梁松.中国沿海赤潮[M].北京:科学出版社,2003.

周名江,李钧,于仁诚,等.赤潮藻毒素研究进展[J].中国海洋药物,1999,71:48-54.

周名江,颜天,傅萌,等.塔玛亚历山大藻对海产双壳类生命活动的影响[J].海洋学报,2004,26(2):81-86

颜天,谭志军,李钧,等.塔玛亚历山大藻和赤潮异弯藻对黑褐新糠虾和卤虫的急性毒性作用[J].中国海洋大学学报,2004,26(1):76-81.

林元烧.有毒甲藻——塔玛亚历山大藻在厦门地区虾塘引起的赤潮[J].台湾海峡,1996,15(1):16-18.

GU Hai-feng,,LAN Dong-zhao,FANG Qi,et al.Cyst formation,development of Alexandrium tamarense from Yangtse River estuary and its relation to bloom dynamics[J].Acta Botanica Sinica,2004,46(9):1 025-1 031.

KODAMA M,OGATA T,SATO S.Bacterterial production of saxitoxin[J].Agric.Biol Chem,1998,52:1 075-1 077.

YOSHIDA T,KAZUMARU M,TERERUKI C,et al.Purification and characterization of sulfotransferase specific to 0-22 of 11-hydroxy saxitoxin from the toxic dinoflagellate Gymnodinium catenatum (dinophyceae)[J].Fisheries Science,2002,6:634-642.

林伟,周名江.有毒藻产毒过程中海洋细菌的作用[J].海洋科学,2001,25(3):34-38.

ZHANG Z D,GREEN B,CAVALIER-SMITH T.Single gene circles in dinoflagellate chloroplast genomes[J].Nature,1999,400:155-159.

陈其军,肖玉梅,王学臣,等.植物功能基因组研究中的基因敲除技术[J].植物生理学通讯,2004,40(1):121-126.

黄健,唐学玺,宫相忠,等.海洋微藻基因工程的选择标记[J].植物学报,2000,42(8):841-844.

徐轶肖,江天久,吕颂辉.有毒赤潮甲藻塔玛亚历山大藻(香港株Ⅱ)的生长特性研究[J].热带亚热带植物学报,2005,13(1):21-24.

J.萨姆布鲁克,D.W 拉塞尔.分子克隆实验指南(第三版)[M].金冬雁等译.北京:科学出版社,2002.8:1 599.

王镜岩,朱圣庚,徐长法.生物化学[M].北京:高等教育出版社,1991.10.

张亚萍,于文功,戴继勋,等.条斑紫菜叶状体细胞的抗生素敏感性研究[J].青岛海洋大学学报,2002,32(2):245-250.

于道展,杨玲玲,姜鹏,等.裙带菜基因工程选择标记的研究[J].高技术通讯,2003,6:74-77.

耿德贵,王义琴,李文斌,等.杜氏盐藻基因工程选择标记的研究[J].生物技术,2001,11(5):1-3.

臧晓南,张学成,王高歌,等.节旋藻和螺旋藻对7种抗生素敏感性的比较研究[J].海洋学报,2004,26(3):82-92.

PORSCH P.The nonradioactive chloramphenical acetyltransferase enzyme-linked immunosorbent assay test issuited for promoter activity studies in plant protoplasts[J].Anal Biochem,1993,211:113-116.

汪川,樊天,李海辉,等.质粒载体pMG36e在宿主菌中的遗传稳定性研究[J].卫生研究,2005,34(2):214-216.

杨劲松,赵南芸,邹轩,等.肠球菌中抗生素抗性传递的研究[J].海峡预防医学杂志,1998,4(1):12-14.

WANG Cuan-hong,ZHAO Ming-zhen,YU Han-hai,et al.Effect of antibiotic treatment on toxin production by Alexandrium tamarense[J].2003,16(2):340-347.

施引文献

资源附件(0)

访问统计