几株原甲藻核糖体大亚基RNA基因的部分克隆及序列分析

王波; 米铁柱; 吕颂辉; 孙军; 李荣秀; 于志刚

几株原甲藻核糖体大亚基RNA基因的部分克隆及序列分析

1.
中国海洋大学, 山东, 青岛, 266003
2.
暨南大学, 水生生物研究所, 广东, 广州, 510632
3.
中国科学院, 海洋研究所, 山东, 青岛, 266071
4.
上海交通大学, 上海200030

基金项目: 国家高技术研究发展计划(863计划)(2001AA635090,2004AA639780);国家重点基础研究发展规划(973)(2001CB409704);国家自然科学基金(40306025)

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出版历程
- 收稿日期: 2005-03-22
- 修回日期: 2005-11-03

Cloning and analysis of 28S rDNA partial sequences of several strains of Prorocentrum

1.
Ocean University of China, Qingdao 266003, China
2.
Institute of Hydrobiology, Jinan University, Guangzhou 510632, China
3.
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
4.
Shanghai Jiaotong University, Shanghai 200030, China

摘要

摘要: 对7株赤潮原甲藻28S rDNA 5'端部分序列进行扩增、克隆和序列测定,并从GeneBank上获取14个原甲藻28S rDNA序列,用NJ法和ME法构建了原甲藻属的系统树,并对序列进行分析.结果表明,7株原甲藻28S rDNA扩增序列长度为950~958 bp,通过NJ法和ME法构建的系统树完全一致.大部分分离自不同海域的同种原甲藻的序列高度保守,而不同种间在序列高变区却有较大的差异.但来自南海海域的海洋原甲藻(Prorocentrum micans)与分离自其他海域的株系序列差异较大,甚至超出了有些种间的差异.由28S rDNA高变区获得的序列,有望成为浮游植物特异性分子探针设计的良好靶区域.
- 原甲藻属 /
- 28SrDNA /
- 系统分析
Abstract: The 28S rDNA partial sequences of several strains of Prorocentrum were cloned and sequenced, and other 14 corresponding sequences of Prorocentrum were obtained from Genbank.Then the phylogenetic trees of Prorocentrum were constr ucted with the methods of NJ(neighbour-joining) and M E(minimume-volution).The results demonstrated that the sequenced partial 28S rDNA of 7 strains was 950~958 bp.The phylogenetictree based on the NJ method was similar with that based on the ME method.The sequences of the different strains within the same species are highly conservative;other wise, the diversity among the sequences from different species was obvious.P.micans separated from the South China Sea was very different from the P.micans strains separated from other area of the world, and the dist ances between them were even long erthan the distances between two species.The sequences from the 28S rDNA would be helpful to design species-specific probes of the phytoplankton.
- Prorocentrum /
- 28S rDNA /
- phylogenetic analysis

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