溶藻弧菌对大黄鱼表皮黏液的黏附特性研究

鄢庆枇; 陈强; 马甡; 庄峙厦; 王小如

溶藻弧菌对大黄鱼表皮黏液的黏附特性研究

1.
集美大学水产学院, 福建厦门 361021;厦门大学化学化工学院, 福建厦门 361005
2.
集美大学水产学院, 福建厦门 361021;中国海洋大学水产学院, 山东青岛 266003
3.
中国海洋大学水产学院, 山东青岛 266003
4.
厦门大学化学化工学院, 福建厦门 361005

基金项目: 国家863计划项目(2002AA639600);福建省自然科学基金(B0410022)

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出版历程
- 收稿日期: 2005-09-27
- 修回日期: 2006-03-30

Characteristics of adhesion of Vibrio alginolyticus to the skin mucus of Pseudosciaena crocea

1.
Fisheries College of Jimei University, Xiamen 361021, China;College Chemistry and Chemical Engineering, xiamen University Xiamen, 361005, China
2.
Fisheries College of Jimei University, Xiamen 361021, China;Fisheries College, Ocean University of China, Qingdao 266003, China
3.
Fisheries College, Ocean University of China, Qingdao 266003, China
4.
College Chemistry and Chemical Engineering, xiamen University Xiamen, 361005, China

摘要

摘要: 采用3H-TdR同位素示踪方法研究了溶藻弧菌对大黄鱼表皮黏液的黏附机制.试验结果表明溶藻弧菌经营养饥饿、热处理、抗体处理、蛋白酶及高碘酸处理后黏附作用明显下降;在8种碳水化合物的干扰下溶藻弧菌的黏附作用会有一定程度的变化,其中葡萄糖、果糖、甘露糖能明显促进溶藻弧菌的黏附作用;溶藻弧菌对大黄鱼表皮黏液中较大分子量的物质有较强的亲和力.这些结果表明溶藻弧菌对大黄鱼表皮黏液存在特异性黏附作用,这种黏附与细菌活力、其表面的蛋白类、糖类等物质以及黏液的化学组成都有密切关系.
- 溶藻弧菌 /
- 黏附作用 /
- 大黄鱼 /
- 黏液
Abstract: Characteristics of adhesion of Vibrio alginoly ticus to the skin mucus of Pseudosci aena crocea had been investig at ed by isotope tracer.The results showed that:adhesion of V.alginoly ticus was inhibited evidently by starvation,heat treatment,antibody,protease,periodic acid;bacterial adhesion was influenced by 8 carbohy drates,among them,glucose,fructose,mannose could enhance the adhesion of V.alginoly ticus;V.alginoly ticus has a strong affinity to higher molecular mass component of skin mucus.The results indicated that V.alginoly ticus adhered to the skin mucus of P.crocea by specific adhesion,this adhesion had closely relation to superficial protein,saccharide and activity of the bacteria as well as the chemical component of skin mucus.
- Vibrioalginoly ticus /
- adhesion /
- Pseudosciaena crocea /
- mucus

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