赤潮异弯藻抗体的制备及酶联免疫检测方法的建立

亓海刚; 米铁柱; 辛泽毓; 李荣秀; 于志刚

赤潮异弯藻抗体的制备及酶联免疫检测方法的建立

1.
中国海洋大学, 生命科学与技术学部, 山东, 青岛, 266003
2.
中国海洋大学, 环境科学与工程学院, 山东, 青岛, 266003
3.
中国科学院, 上海生命科学研究院, 上海, 200233
4.
上海交通大学, 生命科学技术学院, 上海, 200030
5.
中国海洋大学, 化学化工学院, 山东, 青岛, 266003

基金项目: 国家高技术研究发展计划(863计划)资助(2001AA635090;2004AA639780);山东省优秀中青年科学家科研奖励基金(01BS08)

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出版历程
- 收稿日期: 2005-05-29
- 修回日期: 2006-01-08

The preparation of polyclonal antibodies against Heterosigma akashiwo and development of an enzyme-linked immunosorbent assay for detecting the alga

1.
Division of Life Science and Technology, Ocean University of China, Qingdao 266003, China
2.
Environmental Science and Engineering College, Ocean University of China, Qingdao 266003, China
3.
Shanghai Institute of Biological Sciences, Chinese Academy of Sciences, Shanghai 200233, China
4.
College of Life Science and Technology, Shanghai Jiao Tong University, Shanghai 200030, China
5.
Chemistry and Chemical Engineering College, Ocean University of China, Qingdao 266003, China

摘要

摘要: 赤潮异弯藻(Heterosigma akashiwo)是一种广泛分布于世界近岸海域的有害针胞藻类(Raphidophyte),也是我国近海常见的赤潮生物,曾经有多次引发赤潮的报道^[1,2].在赤潮发生的早期,自然水体中赤潮生物的细胞密度相对较低,且赤潮藻个体微小,传统的鉴定方法,即海水样品采集后利用光学显微镜直接观察,主要用于藻的定性和分离,当样品量较多时,定量计数过程则烦琐、费时、费力,工作量大.
- 赤潮异弯藻 /
- 抗体 /
- 效价 /
- 特异性 /
- 酶联免疫检测
- Heterosigma akashiwo /
- antibody /
- titer /
- specificity /
- ELISA

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参考文献(13)

颜天,周名江,钱培元.赤潮异弯藻Heterosigma akashiwo的生长特性[J].海洋与湖沼,2002,33(2):209-214.

郭玉洁.大连湾赤潮生物——赤潮异弯藻[J].海洋与湖沼,1994,25(2):211-215.

BATES M,LEGER C,KEAFER B A,et al.Discrimination between domoic-acid-producing and non-toxic forms of the diatom Pseudonitzchia pungens using immunofluorescence[J].Marine Ecology Progress Series,1993,100:185-195.

ANDERSON D M,KEAFER B A,KULIS D M,et al.An immunofluorescent survey of the brown tide chrysophyte Aureococcus anophagefferens along the northeast coast of the United States[J].Journal of Plankton Research,1993,15(5):563-580.

MENDOZA H,LOPEZ-RODAS V,GONZTIEZ-Gil S,et al.The use of polyclonal antisera and blocking of antibodies in the identification of marine dinoflagellates:species-specific and clone-specific antisera against Gymnodinium and Alexandrium[J].Journal of Experimental Marine Biology and Ecology,1995,186(1):103-115.

CARON D A,DENNETT M R,MORAN D M,et al.Development and application of a monoclonal-antibody technique for counting Aureococcus anophagefferens,an alga causing recurrent brown tides in the Mid-Atlantic United States[J].Applied and EnvironMental microbiology,2003,69 (9):5 492-5 502.

米铁柱,甄毓,于志刚,等.海洋浮游藻类检测技术发展及其展望[J].高技术通讯,2003,13(3):96-100.

GUILLARD R R L,RYTHER J H.Studies on marine planktonic diatoms:I.Cyclotella nana (Hustedt) and Detonula confervaceae(Cleve)[J].Canadian Journal of Microbiology,1962,8:229-239.

NAKANISHI K,KARUBE I,HIROSHI S,et al.Detection of the red tide-causing plankton Chattonella marina using a piezoelectric immunosensor[J].Analytica Chimica Acta,1996,325(1-2):73-80.

杨利国,胡少昶,魏平华,等.酶免疫测定技术[M].南京:南京大学出版社,1998.441-442.

COSTAS E,LOPEZ-RODAS V.Enumeration and separation of the toxic dinoflagellate Alexandrium minutum from natural samples using immunological procedures with blocking antibodies[J].Journal of Experimental Marine Biology and Ecology,1996,198 (1):81-87.

SCHOLIN C A,MARIN R Ⅲ,MILLER P E,et al.DNA probes and a receptor binding assay for detection of Pseudo-nitzschia(Bacillariophyceae)species and domoic acid activity in cultured and natural samples[J].Journal of Phycology,1999,35:1 356-1 367.

LIN S J,FEINSTEIN T F,ZHANG H,et al.Development of an immunofluorescence technique for detecting Pfiesteria piscicida[J].Harmful Algae,2003,2(3):223-231.

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