热带太平洋深海微生物的若干生理生态特征

骆祝华; 裴耀文; 黄翔玲; 叶德赞

热带太平洋深海微生物的若干生理生态特征

国家海洋局, 第三海洋研究所, 福建, 厦门, 361005;国家海洋局海洋生物遗传资源重点实验室, 福建, 厦门, 361005

基金项目: 中国大洋矿产研究开发协会资助项目(DY105-02-03);国家重点基础研究专项经费资助项目(G2000078500);国家海洋局青年海洋科学基金资助项目(2003111)

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出版历程
- 收稿日期: 2005-05-24
- 修回日期: 2005-12-25

Physiological and ecological characteristics of the deep-sea microorganisms in tropical Pacific Ocean

The Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China;The Key Laboratory of Marine Biogenetic Resource of State Oceanic Administration, Xiamen 361005, China

摘要

摘要: 从2001年春、秋季于热带太平洋区采集到的深海样品中共分离到110株微生物,从中挑取31株进行形态特征和对环境理化因子适应特征的研究,同时利用Biolog微生物自动鉴定系统进行菌种鉴定和碳源代谢分析.结果表明:31株微生物中2株为酵母,21株为革兰氏阳性细菌,8株为革兰氏阴性细菌,来自底质的细菌菌株阳性菌占优势,而海水、蓝藻中阴性菌居多;温度、盐度、pH适应性试验表明,严格意义上的狭适性微生物不到总数的1/3,而具有较宽生态幅的广适性微生物占有相当大的比例.另一方面,耐冷菌、中度嗜盐菌和抗碱菌在这些微生物中的比例很高,同时,来自蓝藻的菌株大多数在4℃下不生长,而来自上覆水和底质的菌株可在4℃生长,这些都体现了微生物对生存环境的响应.31株微生物经Biolog鉴定12株得出鉴定结果.15株革兰氏阳性细菌对95种碳源的利用能力分析表明,不同菌株可利用碳源的数目从3种到46种不等,差异很大,体现了深海微生物营养类型的极大多样性;同时,发现有8种碳源可被一半以上的受试菌株所利用,这些碳源均是微生物各类代谢途径中的重要中间代谢物.
- 热带太平洋 /
- 深海微生物 /
- 生理生态特征 /
- Biolog微生物自动鉴定系统
Abstract: 31 cultures of the 110 microbial strains, which were isolated from deep-sea samples taken in tropical Pacific Ocean in Summer and Autumn, 2001, were selected for this research.The characters of their morphologies and of their adapting to the physical and chemical factors were studied, and the identification of these strains and their carbon-utilizing patterns were performed by Biolog Microstation System.The results demonstrated that the tested strains consisted of 2 yeasts, 21 Gram-positive bacteria and 8 Gram-negative bacteria, of which the Grampositive bacteria were dominant in the sediment samples while the Gram-negative bacteria were in the majority in the samples of sea waters and blue-greenalgaes.The adaptability of these st rains for different environmental factors (temperature, salinity and pH) was studied and it was found that less than 1/3 of the tested strains showed stenotropic adaption to the environmental factors, while a large proportion of the cultures was eurytropic microo rganisms with wideecological amplitude.On the other hand, among these microo rganisms, psycho trophs, moderate halophiles and alkalit olerant bacteria were in very high percentages.Furthermore most of the strains from blue-greenalgae samples could not,but those from over-laid water and sediment samples were able to, grow at 4.All these results incarnated the response of microorg anisms to their living environments.12 strains of the to taltested microo rganisms could be given the taxonomic results by the database of Biolog Microstation System.According to the carbon-utilizing spectra of the 15 strains of Grampositive bacteria vs 95 types of organic carbons in Boilog GP 2 Microplates, the numbers of the utilizable carbon sources showed a greatrange from 3 to 46 single organic carbons for each strain, embodying the great diversity of nutrition types in deep sea microorg anisms.Among the utilizable carbons, 8 types could be used by more than half of the tested Gram-positive bact eria.These organic carbon so urces are the important intermediatemet abolite in all kinds of metabolic pathway for microorg anisms.
- tropical Pacific Ocean /
- deep-sea microorganisms /
- physiological and ecological characteristics /
- Biolog Microstation System

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参考文献(24)

D'HONDT S,RUTHERFORD S,SPIVACK A J.Metabolic activity of subsurface life in deep-sea sediments[J].Science,2002,295:2 067-2 070.

WHITMAN W B,COLEMAN D C,WIEBE W J.Prokaryotes:the unseen majority[J].Proc Natl Acad Sci USA,1998,95:6 578-6 583.

DUCKLOW H W.The bacterial component of the oceanic euphotic zone[J].FEMS Microbiol Ecol,1999,30:1-10.

LI L,KATO C,HORIKOSHI K.Bacterial diversity in deep-sea sediments from different depths[J].Biodivers Conserv,1999,8:659-677.

SASS A M,SASS H,COOLEN M J,et al.Microbial communities in the chemocline of a hypersaline deep-sea basin (Urania basin,Mediterranean Sea)[J].Appl Environ Microbiol,2001,67:5 392-5 402.

INAGAKI F,SAKIHAMA Y,INOUE A,et al.Molecular phylogenetic analyses of reverse-transcribed bacterial rRNA obtained from deep-sea cold seep sediments[J].Environ Microbiol,2002,4:277-286.

SIEVERT S M,BRINKHOFF T,MUYZER G,et al.Spatial heterogeneity of bacterial populations along an environmental gradient at a shallow submarine hydrothermal vent near milos island (Greece)[J].Appl Environ Microbiol,1999,65:3 834-3 842.

李崇银,穆明权.东亚冬季风-暖池状况-ENSO循环的关系[J].科学通报,2000,45(7):678-685.

蔡怡,王彰贵,余宙文,等.赤道太平洋温度、流场距平EOF分析及与厄尔尼诺的关系[J].海洋学报,2003,25(1):12-18.

陈幸荣,王彰贵.西风爆发、次表层暖水东移与厄尔尼诺现象[J].海洋学报,2003,25(1):19-27.

梁建茵,吴尚森.太平洋海温异常对南海西南季风建立早晚的影响——数值模拟研究[J].海洋学报,2003,25(1):28-41.

张启龙,翁学传,侯一筠,等.西太平洋暖池表层暖水的纬向运移[J].海洋学报,2004,26(1):33-39.

周德庆.微生物学实验手册[M].上海:上海科学技术出版社,1986.

HY/T 003.9-91,海洋监测规范:近海污染生态调查和生物监测[S].

GREGERSEN T.Rapid method for distinction of Gram-negative from Gram-positive bacteria[J].Eur J Appl Microbiol Biotechnol,1978,5:123-127.

SEIBURTH J M.Sea microbial seascapes[M].Baltimore:University Park Press,1979.

BOWMAN J P,REA S M,McCAMMON S A.Diversity and community structure within anoxic Hilds,Eastern Antarctic[J].Environ Microbiol,2000,2:227-237.

RAVENSCHLG K,SAHM K,AMANN R.Quantitative molecular analysis of the microbial community in marine Arctic sediments(Svalbard)[J].Appl Environ Microbiol,2001,67:387-395.

WEBSTER N C,WILSON K J,BLACKALL L L,et al.Phylogenetic diversity of bacteria associated with the marine sponge Rhopaloeides odorabile[J].Appl Environ Microbiol,2001,67:434-444.

李越中,陈琦.海洋微生物资源多样性[J].生物工程进展,1998,18(4):34-40.

沈国英,施并章.海洋生态学[M].北京:科学出版社,2002.

SMALLA K,WACHTEDORE U,HEUER H,et al.Analysis of BIOLOG GN substrate utilization patterns by microbial communities[J].Appl Environ Microbiol,1998,64:1 220-1 225.

GAMO M,SHOJI T.A method of profiling microbial communities based on a most-probable-number assay that uses BIOLOG plates and multiple sole carbon sources[J].Appl Environ Microbiol,1999,65:4 419-4 424.

刘志恒.现代微生物学[M].北京:科学出版社,2002.

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