盐度和pH对底栖硅藻胞外多聚物的影响
Production of extracellular polymeric substances(EPS) by benthic diatom: effect of salinity and pH
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摘要: 研究了盐度和pH值对底栖硅藻新月筒柱藻(Cylindrotheca closterium(Ehr.)Reimannet Lewin)增殖、蛋白质含量和胞外多聚物(Extracellular Polymeric Substances,EPS)的影响.结果表明新月筒柱藻最适生长的盐度和pH值分别是15和8,属半咸水性生活.高盐度(>15)和低pH值(<pH8)的胁迫促进了胞外多聚物(EPS)的积累,说明EPS的存在可能有利于缓解外界的不利条件.胶体EPS和附着EPS对盐度和pH值的响应不同,反应了两种EPS功能上的差异.盐度和pH值对新月筒柱藻胞内碳水化合物的影响不显著.Abstract: Influences of salinity and pH value on reproduction, protein content and extracellular polymeric substances (EPS) of benthic diatom Cylindrotheca closterium (Ehr.) Reimannet Lewin were studied.Salinity and pH value fit to C.closterium were 15 and 8, respectively, showing brackish water species.Abundant EPS were secreted by C.closterium characterized by brackish water species under high salinity (> 15) and low pH (< 8) stress, showing functions in abnormal conditions.The fraction of EPS that was closely bound to the cells (at tached EPS) and the soluble fraction (colloidal EPS) were produced in differentam ounts at the different salinity and pH value.This suggested that the production of the 2 operationally defined fractions of EPS might serve different functions.No significant differences were found on intracel lular carbohydrate production of C.closterium under different salinity and pH value.
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Key words:
- benthic diatoms /
- extracellular polymeric substances (EPS) /
- salinity /
- pH
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