外源激素对养殖牙鲆血浆睾酮和雌二醇含量的影响研究

温海深; 宋海霞; 杨立廷; 马学坤; 高玲

外源激素对养殖牙鲆血浆睾酮和雌二醇含量的影响研究

1.
中国海洋大学, 水产学院, 山东, 青岛, 266003
2.
中国海洋大学, 水产学院, 山东, 青岛, 266003;国家海洋局第三海洋研究所, 福建, 厦门, 361005
3.
青岛大学, 医学院附属医院, 山东, 青岛, 266071

基金项目: 中国博士后科学基金资助(2002031167)

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出版历程
- 收稿日期: 2005-10-18
- 修回日期: 2005-12-10

Study on the effects of exogenous hormone on the plasma testosterone and estradiollevels in cultured Japanese flounder

1.
Fisheries College, Ocean University of China, Qingdao 266003, China
2.
Fisheries College, Ocean University of China, Qingdao 266003, China;Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China
3.
Appertain Hospital of Medicine College, Qingdao University, Qingdao 266071, China

摘要

摘要: 雄性牙鲆单独注射促黄体激素释放激素类似物(LHRH-A)(每千克质量的注射量,下同)25μg/kg或人绒毛膜促性腺激素(hCG)1 000 IU/kg,血浆睾酮(T)含量在注射后6 h大幅度降低,12h时达到最低值,以后逐渐回升;LHRH-A与hCG联合注射[LHRH-A(12.5μg/kg)+hCG(500 IU/kg)],注射后6 h血浆T含量剧烈下降,24 h时达到最低点,以后缓慢回升;对照组雄鱼血浆T含量随着采样进行而呈现降低趋势.雌鱼单独LHRH-A后,血浆雌二醇(E₂)水平逐渐下降,24 h时达到最低值,30 h后略有回升;单独注射hCG后,血浆E₂含量缓慢下降,30 h达到最低值;LHRH-A与hCG联合注射,注射后6 h血浆E2含量逐渐下降,24 h时达到最低点,30 h时缓慢回升.对照组雌鱼血浆E₂水平也随着采样的进行而逐渐降低.结果表明:单独或联合注射LHRH-A和hCG降低了雌、雄牙鲆血浆E₂和T含量,与对照组结果相同.
- 牙鲆 /
- 性激素 /
- 生殖
Abstract: When male Japanese flounders were injected with alone luteinizing hormone-releasing hormone analogue(LHRH-A) (25 g/kg) or human chorionic goandotropin(hCG) (1 000 IU/kg), plasma testosterone(T) levels decreased significantly post injection 6 hour, and occurred the lowest levels post injection 12 hour, and somewhat increased postinjection 24 to 30 hour.Plasma T content declined abruptly postinjection 6 hour and appeared the least value of plasma T post injection 24 hour in that male fish injected with LHRH-A plushCG (12.5 g/kg+500IU/kg).At the same time, the plasma T levels of male fish in control group also decreased as these fish treated with LHRH-A or hCG.The plasma estradiol(E₂) levels decreased significantly post injection 6 hour, and occurred the lowest levels post injection 24 hour, and somewhat increased post injection 30 hour, when female fish were injected with alone LHRH-A, whereas, the least plasma E₂ levels occurred post injection 30 hour in that female fish injected with alone hCG.Plasm a E₂ content decreased gradually post injection 6 hour and appeared the least value of plasma E₂ postinjection 24 hour in that female fish injected with LHRH-A plus hCG.On the mean while, the plasma estradiol levels of the fish in control group also declined as these fish treated with LHRH-A or hCG.These results suggestion that the injection with alone LHRH-A or hCG or combined LHRH-A and hCG failed to increase significantly plasma testosteroneand estradiollevels, on the contrary, suppressed testosteroneand estradiolconcent ration in male and female Japanese flounder.
- Japanese flounder /
- Steroid /
- Reproduction

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