谷氨酸铜和表面活性剂协同灭除亚历山大藻的研究
Complex extinguishment of harmful algae by cupric glutamine and surfactant
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摘要: 对一种新的赤潮杀灭剂谷氨酸铜及促进剂阳离子表面活性剂十六烷基三甲基溴化铵(HDT-MAB)进行了初步研究.比较了硫酸铜及谷氨酸铜对亚历山大藻的去除效果;结果表明,谷氨酸铜对亚历山大藻的灭杀效率优于硫酸铜,谷氨酸铜可明显抑制亚历山大藻细胞的生长,但抑制作用并不随谷氨酸铜浓度加大而增强,HDTAMB可明显促进谷氨酸铜对亚历山大藻的去除,且促进作用基本随着HDTMAB浓度的提高而增强.对谷氨酸铜和HDTMAB杀灭赤潮的机制进行了初步探讨;研究发现,谷氨酸铜可促使藻细胞MDA含量升高,并使巯基含量下降,且在谷氨酸铜和HDT-MAB复合作用下,膜脂过氧化水平大大高于单纯谷氨酸铜作用下的水平,细胞中巯基含量显著降低,细胞膜透性增大,这表明谷氨酸铜和HDTMAB杀灭赤潮的机制是使藻细胞膜受到损伤,进而抑制藻细胞生长.Abstract: Cupric glutamate was studied as a novel algicide formarine harmful algae and hexadecy ltrimethyl-eamine bromide(H DT MAB)as an accelerant.The studies on the extinguishment of Alexandrium sp.by cupric sulfate or cupric glutamate showed that cupric glutamate had a higher exting uishing rate than cupric sulfate.Cupric glutamate had an excellent ability to inhibit the growth of Alexandrium sp.,but the inhibition efficiency did not increase with higher cupric glutamate concentration.HDTMAB could stimulate the exting uishments by cupric glutamate obviously.There was a positive correlation between concentration of HDTMAB and increasing effect.Study of the mechanism of the exting uishment of Alexandrium sp. showed that cupric glutamate stimulated the production of MDA and reduced the content of sulf hydryl group.The lipid peroxidation level in cells was higher under cupric glutamate-surfact antjoint operation than cupric glutamate alone.In addition of the content of sulfhydryl group decreased significantly,plasma membrane permeability of leaked cell solution was increased.These indicated the extinguishing mechanism:the cell membrane was damaged followed by the inhibition of the cell growth.
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Key words:
- cupric glutamate /
- surfactant /
- Alexandrium sp. /
- lipid peroxidation
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