斑纹鲟凝集素(CFL)的初步研究 Ⅱ.CFL的分子组成及性质鉴定
The preliminary studies of a lectin(CFL) from Charybdis feriatus Ⅱ.The molecular composition and the characterization of CFL
-
摘要: 采用生化方法对斑纹鲟(Charybdis feriatus)凝集素进行了初步研究.结果表明,斑纹鲟的血清中有动物红细胞和微生物细胞凝集活性的凝集素(Charybdis feriatuslectin,CFL),其活力表现依赖于Ca2+,并为多种糖所抑制;CFL对40℃以上的温度敏感,其活力在pH6.0~8.0内稳定.经巯基乙醇处理和未经巯基乙醇处理的CFL在SDS-PAGE上均为一条蛋白带,说明斑纹鲟凝集素不含二硫键,且只有一种亚基,分子量为40.8 kDa,CFL氨基酸组成分析表明其富含酸性氨基酸.
-
关键词:
- 斑纹鲟凝集素(CFL) /
- 糖抑制 /
- 热稳定性 /
- 分子量 /
- 氨基酸组成
Abstract: Lectin of Charybdis feriatus(shortly call CFL)was studied by biochemistry methods.The results show that the lectin agglutinates every kinds RBC from different animals and microbial cells tested widely in the experiment,as a Ca2+ dependent lect in CFL is inhibited by various sugars.CFL is sensitive to the temperature over 40 e and to the pH over 9.0 or below 5.0.No matter adding or not adding-S-S-reducant to the sample of CFL,it demonstrated one single band on plate SDS-PAGE.It shows that CFL does not contain-S-S-,and its molecular mass is 40.8kDa.The analysis result of aminoacid composition of CFL indicates that acidity amino acids were abundant. -
BARKAI-GOLANT,SHARON N.Lectins as a tool for the study of yeast cell walls[J].Exp Mycol,1978,2:110-113. GOLDSTEIN I J,HAYES C E.The lectins carbohydrate-binding proteins of plants and animals[J].Carbohydr Chem Biochem,1978,35:127-340. KILPATRCK D C,VANDRIESSCHE E,POOG-HANSEN T C.Lectin reviews[M].Louis:Sigma Chemical Co,1991.1:28 LIENER I E,SHARON N,GOLDSTEIN I J.The Lectins:Properties,Functions,and Applications in Biology and Medicine[M].San Diego:Academic Press,1986. PARRINELLO N.Animal lectins and immune recognition:a phylogentic approach:form and function in zoology[J].Mucci Editore,Modena,1991,5:43-68. RENWRANTZ L.Lectins in molluscs and arthropods:their occurrence,origin and roles in immunity[J].Symp Zool Soc London,1986,56:81-93. VASTA G R.Invertebrate lectins:distribution,synthesis,molecular biology,and function[A].Al-len H J,KISALIUS E C.Glycoconjugates,Composition,Structure and Function[M].New York:Marcel Dekker,1992.593-634. OLAFSEN J A.Lectins:models of natural and induced molecules in invertebrates[J].Adv Comp Environ Physiol,1996,24:49-76. JAMES L H,DAVID T,ROWLAND J.Heterogeneity of lobster agglutinins:Ⅱ.Specificity of agglutinin-erythrocytes binding[J].Biochemistry,1974,13 (4):828-832. JAMES L H,DAVID T,ROWLAND J.Heterogeneity of lobster agglutinins:Ⅰ.Purification and physiochemical characterization[J].Biochemistry,1974,13(4):821-827. MURAMOTO K,KADO R,TAKEI Y,et al.easonal changes in the mulitiple lectin compositions of the acorn barnacle megabalanus rosa as related to ovarian development[J].Comp Biochem Phsiol,1991,98B(4):603-607. MURAMOTO K,YAKO H,KAMIYA H.Mulitiple lectins as major proteins in coelomic fluid of the acron barnacle megabalanus rosa[J].Comp Biochem phsiol,1994,107B(3):405-409. MURAMOTO K,YAKO H,MACAKAMI K,et al.Inhibition of the growth of calcium carbonate crystals by multiple lectins in the coelomic fluid of the acron barnacle megabalanus rosa[J].Comp Biochem Phsiod,1994,107B(3):401-409. 罗日祥.中国对虾凝集素活力及弧菌的诱导动力学[J].海洋学报,1997,19(4):117-120. 刘志鸿,张士璀,杨爱国,等.毛蚶血清凝集素的凝集活性[J].海洋学报,2003,25(5):92-96. SHARON N,LISH H.Lectins:cell agglutinating and sugar-specific proteins[J].Science,1964,146(23):548-549. 梁宋平,林莉.虎纹捕鸟蛛凝集素-Ⅰ(SHL-Ⅰ)的细胞凝集活性分析[J].中国生物化学与分子生物学报,2000,16(1):92-95. 胡新平,李智恩,徐祖洪.海藻凝集素研究进展[J].海洋科学,2000,24(8):34-37. TAMPLIN M L,FISHER W S.Occurrence and characteristics of agglutination of Vibrio cbolerae by serum from the eastern oyster[J].Crassostrea Virrginica Appl Eniron,Microbiol,1989,55(11):2 882-2 887. PITOLE T G.Interaction of bacteria and fungi with lectins and lectin-like substance[J].ANN Rev Microbiol,1981,35:85-112.
点击查看大图
计量
- 文章访问数: 739
- HTML全文浏览量: 4
- PDF下载量: 754
- 被引次数: 0