生物胺对锯缘青蟹精巢发育的调控作用
Effects of biogenic amines on the testicular development in male mud crabs, Scylla serrata
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摘要: 采用活体注射和体外培养方法,以精巢指数、精巢生精小管的成熟节段比例和促雄腺B型细胞比例为量化指标,观察3种生物胺对雄性锯缘青蟹生殖神经内分泌的调控作用.活体注射实验表明:5-HT组锯缘青蟹精巢发育和促雄腺分泌活动具有显著的促进作用;DA组精巢发育受到抑制,但促雄腺细胞的分泌活动不受影响;OA组与同步对照组差异不显著.离体实验表明:5-HT刺激了脑和胸神经团的分泌活动,从而促进促雄腺分泌活动;OA和DA对脑和胸神经团分泌活动没有明显影响.视神经节的生殖内分泌活动几乎不受3种生物胺的影响.本实验首次报道了体外条件下生物胺对雄性甲壳动物生殖神经内分泌的调控作用,支持了5-HT首先刺激脑和胸神经团分泌GSH,再通过促雄腺活动从而促进精巢发育的观点.Abstract: The regulation of three biogenic amines over the reproductive neuroendocrine activity of male Scylla serrata was investigated by invivo injection and invitro incubation.The testicular index,the ratio of the mature sections among the seminiferous tubules,and the ratio of Type Bcells among the androgenic glandular cells were taken as the quantitative indexes.In vivo injections indicated that:5-HT can significantly promote the testicular development and the secretion of the androg enic gland in S.serrata;DA can inhibit the testicular development,but no influence on the secretion of the androgenic gland is found;no significant difference was observed between the OA-injected group and the concurrent control.In vitro incubations showed that:5-HT can stimulate the secretion of the brain and the thoracic ganglia,thus accelerate that of the androgenic gland;in the OA and the DA groups,however,no significant influence on the secretion of the brain and the thoracic ganglia was observed.As to the optic ganglia,the three biogenic amines hardly have any effects on the secretion.It is the first time for invitro experiments to report the regulation of biogenic amines over the reproductive neuroendocrine of male crustaceans.Our results corrobo rate that 5-HT first activate the brain and the thoracic ganglia to secret GSH,then promote the testicular development through the androgenic gland.
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Key words:
- biogenic amines /
- Scylla serrata /
- neuroendocrine organs /
- androgenic gland /
- reproduction
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FINGERMAN M,NAGABHUSHANAM R,SAROJINI R,et al.Biogenic amines in crustaceans:identification,localization,and roles[J].J Crust Biol,1994,14(3):413-437. FINGERMAN M.Roles of neurotransmitters in regulating reproductive hormone release and gonadal in decapod crustaceans[J].Invert Reprod Develop,1997,31(1～3):47-54. 叶海辉,李少菁,李祺福,等.生物胺对雌性锯缘青蟹生殖神经内分泌活动的调控作用[J].海洋与湖沼,2003,34(3):329-333. SAROJINI R,NAGABHUSHANAM R,FINGERMAN M.5-Hydroxytryptaminergic control of testes development through the androgenic gland in the red swamp crayfish,Procambarus clarkii[J].Invert Reprod Develop,1994,26(2):127-132. SAROJINI R,NAGABHUSHANAM R,DEVI M,et al.Dopaminergic inhibition of 5-hydroxytryptamine-stimulated testicular maturation in the fiddler crab,Uca pugilator[J].Comp Biochem Physiol,1995,111C:287-292. 叶海辉,李少菁,黄辉洋,等.锯缘青蟹精巢发育的组织学研究[J].动物学研究,2002,23(2):141-144. 叶海辉,李少菁,黄辉洋,等.锯缘青蟹促雄腺发育的组织学研究[J].中国水产科学,2003,10(5):376-381. 吴萍,楼允东,邱高峰.甲壳动物雄性腺研究的进展[J].水产学报,1999,23(1):77-83. 叶海辉,李少菁,黄辉洋,等.锯缘青蟹促雄腺进行全浆分泌的证据[J].中国水产科学,2003,10(5):431-433. 黄辉洋,叶海辉,李少菁,等.锯缘青蟹胸神经团的免疫细胞化学研究[J].海洋科学,2003,27(3):77-80. 黄辉洋,叶海辉,李少菁,等.锯缘青蟹视神经节的免疫细胞化学研究[J].海洋学报,2004,26(11):125-129. KULCARNI G K,NAGABHUSHANAM R,AMAIDOSS G,et al.In vivo stimulation of ovarian development in the red swamp crayfish,Procambarus clarkii (Girard),by 5-Hydroxytryptamine[J].Invert Reprod Develop,1992,21(3):231-240. MEYERS D E,CRAF R A,COOKE I M.Ionic currents of morphologically distinct pepetidergic neurons in defined culture[J].J Neurophysiology,1992,67(4):1 301-1 305. 孙金生,刘安西,陈家童,等.河蟹眼柄神经分泌细胞离子通道的膜片钳研究[J].生物物理学报,2001,17(2):235-240. -
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