栉孔扇贝第1卵裂抑制型雌核发育二倍体的研究

潘英; 李琪; 于瑞海; 王如才

栉孔扇贝第1卵裂抑制型雌核发育二倍体的研究

1.
中国海洋大学水产学院山东青岛 266003;广西大学动物科技学院广西南宁 530005
2.
中国海洋大学水产学院山东青岛 266003

基金项目: 国家自然科学基金资助项目(30170735);教育部重点资助项目(104114)

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出版历程
- 收稿日期: 2004-11-18
- 修回日期: 2005-09-21

Gynogenetic diploid induced by suppression of first cleavage of Chlamys farreri

1.
Fisheries College, Ocean University of China, Qingdao 266003, China;College of Animal Science and Technology, Guangxi University, Nanning 530005, China
2.
Fisheries College, Ocean University of China, Qingdao 266003, China

摘要

摘要: 利用6-二甲基氨基嘌呤(60μg/cm³;6-DMAP)和细胞松弛素B(0.5μg/cm³;CB)抑制受精卵的第1次卵裂,授精后60和65 min分别用CB和6-DMAP持续处理20和15 min,可诱导出12.5%和27.5%的第1卵裂抑制型雌核发育二倍体.细胞学观察显示,6-DMAP抑制第1次卵裂产生的雌核发育二倍体主要在第1次有丝分裂后期的受精卵,由于破坏了纺锤体和阻止了染色体分离,导致一个二倍性雌核的形成,而CB有效地阻止了第1次卵裂的胞质分裂.尽管倍化率和D型幼虫发生率较低,但该研究首次报道了栉孔扇贝第1卵裂抑制型雌核发育二倍体诱导的可行性.
- 栉孔扇贝 /
- 雌核发育二倍体 /
- 6-二甲基氨基嘌呤 /
- 细胞松弛素B /
- 细胞学
Abstract: Conditions required for the induction of gynogenetic diploid by suppression of first cleavage of Chlamys farreri were examined using treatments of cytochalasin B (0.5 g/cm³;CB) and 6-dimethylamin-opurine (60 g/cm³;6-DMAP).Treatments with CB and 6-DMAP were effective in inhibiting not only meiosis but alsomitosis,and the optimum time for the suppression of first cleavage began at 60~65 min with 20 and 15 min duration time after insemination.This resulted in 12.5% and 27.5% mitotic gynogenetic diploids (mitotic-G2N) respectively.Cytological observations indicated that 6-DMAP disrupted the spindle at mitosis and inhibited chromosome movement,resulting in the formation of one diploid female nucleus,while CB inhibited the cytokinesis of first cleavage but not the segregation of chromosomes.Although attempts to produce mitotic-G2N demonstrated that ploidy levels and the developmental rate of D-shaped lar-vaearelow,the results of this study suggest the possibility of producing mitotic-G2N Chalamys farreri.
- Chlamys farreri /
- gynogenetic diploid /
- 6-DMAP /
- CB /
- cytology

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参考文献(26)

STREIESINGER G, WALKER C, DOWER N, et al. Production of clones of homozygous diploid zebra fish (Brachydanio rerio)[J]. Nature, 1981, 291(5813):293-296.

HUSSAIN M G. Suppression of first cleavage in the Nile tilapia, Oreochrois niloticus L[A]. Genetics in AquacultureⅣ [C]. Hardbound:Elsevier, 1993. 262-270.

KOMEN J, BONGERS A B J, RICHTER C J J, et al. Gynogenesis in common carp (Cyprinus carpio L.):Ⅱ. The production of homozygous gynogenetic clones and F1 hybrids[J]. Aquaculture, 1991, 92:127-142.

NARUSE K, IJIRI K, SHIMA A, et al. The production of cloned fish in the medaka (Orgzias latipes)[J]. J Exp Zool, 1985, 236(3):335-341.

TABATA K, GORIE S. Induction of gynogenesis diploids in Paralichthys olivaceus by suppression of the 1st cleavage with special reference to their survival and growth[J]. Nippon Suisan Gakkaishi, 1988, 54(11):1 867-1 872.

MA A P H. Gynogenesis induction in the Pacific oyster (Crassostrea gigas Thunberg) using ultraviolet light and cytochalasin B[D]. Seattle:University of Washington, 1987.

GUO Xi-ming, HERSHBERGER W K, COOPER K, et al. Artificial gynogenesis with ultravoilet light-irradiated sperm in the Pacific oyster, Crassostrea gigas:I. Induction and survival[J]. Aquaculture, 1993, 113(3):201-214.

LI Qi, OSADA M, KASHIHARA M, et al. Induction of gynogenetic diploids and cytological studies in the Pacific oyster, Crassostrea gigas[J]. Suisanzoshoku, 2000, 48(2):185-191.

FUJINO K, ARAI K, IWADARE K, et al. Induction of gynogenetic diploid by inhibiting 2nd meiosis in the Pacific abalone[J]. Bull Jap Soc Sci Fish, 1990, 56(11):1 755-1 763.

LI Qi, OSADA M, KASHIHARA M, et al. Artificially induced gynogenetic diploid in the Pacific abalone, Haliotis discus hannai [J].Fish Genet Breed Sci, 1999, 28(1):85-94.

FAIRBROTHER J E. Viable gynogenetic diploid Mytilus edulis (L.) larvae produced by ultraviolet light irradiation and cytochalasin B shock[J]. Aquaculture, 1994, 126(1-2):25-34.

SCARPA J, KOMARU A, WADA K T. Gynogenetic induction in the mussel, Mytilus galloprovincialis[J]. Bull Natl Res Inst Aquac,1994, 23(1):33-41.

SCARPA J, BOLTON E T. Experimental production of gynogenetic and parthenogenetic Mulinia laterlis(Say)[J]. J Shellfish Res,1988,7(1):132.

LI Qi, OSADA M, KASHIHARA M, et al. Gynogenetic diploids induced by suppression of first cleavage in the Pacific oyster, Crassostrea gigas[J]. Fish Genet Breed Sci, 2000, 29(1):37-48.

王梅林,郑家声,余海.栉孔扇贝染色体核型[J].青岛海洋大学学报,1990,20(1):81-85.

NEANT I, GUERRER P. 6-Dimethylaminopurine blocks starfish oocyte maturation by inhibiting a relevant protein kinase activity[J].Exp Cell Res, 1988,176(1):68-79.

DUBE F, DUFRESNE L, COUTU L, et al. Protein phosphorylation during activation of surf clam oocytes[J]. Dev Biol, 1991, 146(2):473-482.

DUFRESNE L, NEANT I, ST-PIERRE J, et al. Effects of 6-dimethylaminopurine on microtubules and putative intermediate filaments in sea urchin embryos[J]. J cell Sci, 1991, 99(4):721-730.

SIMILI M, PELLERANO P, PIGULLO S, et al. 6-DMAP inhibition of early cell cycle events and induction of mitotic abnormalities[J].Mutagenesis,1997, 12(5):313-319.

DESROSIERS R R, GERARD A, PEIGNON J M, et al. A novel method to produce triploids in bivalve mollusks by the use of 6-dimethylaminopurine[J]. J Exp Mar Biol Ecol, 1993, 170(1):29-43.

RIME H, NEANT I, GUERRIER P, et al. 6-Dimethylaminopurine (6-DMAP), a reversible inhibitor of the transition to metaphase during the first meiotic cell division of the oocyte[J]. Der Biol, 1989,133(2):169-179.

NEANT I, CHARBONNEAU M, GUERRER P. A requirement for protein phosphorylation in regulating the meiotic and mitotic cell cycles in echinoderms[J]. Dev Biol, 1989, 132(3):304-314.

SZOLLOSI M S P, DEBEY D, SZOLLOSI H, et al. Chromatin behaviour under influence of puromycin and 6-DMAP at different stages of mouse oocyte maturation[J]. Chromosoma, 1991, 100(5):339-354.

毛连菊,王子臣,刘相全,等.咖啡因加热休克诱导皱纹盘鲍多倍体的研究[J].遗传学报,2000,27(11):959-965.

PAN Ying, LI Qi, YU Rui-hai, et al. Induction of gynogenetic diploids and cytological studies in the zhikong scallop, Chlamys farreri [J]. Aquat Living Resour, 2004, 17(2):201-206.

李冰霞,罗琛.热休克法抑制第一次卵裂实现草鱼雌核发育的细胞学观察[J].水生生物学报,2003,27(2):155-160.

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