海湾扇贝养殖过程中的流行病学调查研究

张维翥; 吴信忠; 李登峰; 孙敬锋; 张扬; 杨霞

海湾扇贝养殖过程中的流行病学调查研究

1.
中国科学院, 南海海洋研究所, 广东广州 510301;滁州学院, 化学与生命科学系, 安徽滁州 239000
2.
中国科学院, 南海海洋研究所, 广东广州 510301;浙江大学, 动物科学学院, 浙江杭州 310029
3.
中国科学院, 南海海洋研究所, 广东广州 510301

基金项目: 国家"973"重点基础发展规划资助项目(G1999012001);国家自然科学基金农业倾斜资助项目(30170741).

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出版历程
- 收稿日期: 2004-10-23
- 修回日期: 2005-05-13

Epizootiological study on mass mortalities of the cultured scallops Argopecten irradians

1.
South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;Biology Department, Chuzhou Normal College, Chuzhou 239000, China
2.
South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;College of Animal Sciences, Zhejiang University, Hangzhou 310029, China
3.
South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China

摘要

摘要: 2001~2002年,对莱州湾养殖海湾扇贝进行了较系统地流行病学调查研究.以海湾扇贝养殖集中海区莱州市金城镇扇贝养殖场为定点调查和样品采集点.调查内容主要包括海湾扇贝各养殖期的死亡率和可疑病原生物检测,同时对pH,溶解氧,温度,盐度等理化环境因子进行连续监测.调查结果显示:海湾扇贝在0.5~1.3cm的幼贝阶段死亡率较高,达50%;随后死亡率降低,1.3~2.2cm的幼贝阶段死亡率约为20%,以后各阶段死亡率平稳,均在7%以下,累积死亡率约为60%.对环境理化因子的调查显示,pH,溶解氧、盐度的变化幅度不大,与扇贝死亡率无明显相关性;温度变化与死亡率有一定的相关性,即低温(20℃以下)可能是养殖海湾扇贝幼贝重要的环境胁迫因子,而高温(27℃左右)对海湾扇贝成贝生长无不良影响.通过对可疑致病性生物因子的调查研究及病理学观察表明,海湾扇贝在养殖过程中可感染多种微生物,其中原核微生物类立克次体(RLO)在养殖过程各生长阶段均可检出,即0.5,1.5,3.0,3.6和5.0cm的海湾扇贝RLO的感染率分别为15%,35%,80%,50%和20%;RLO的感染强度分别为1.0,1.5,16.0,11.5和5.0.在病原生物检查过程中,除RLO以外还发现有类支原体原核生物(MLO)寄生.结合感染强度和组织病理学的相关性分析,初步认为类立克次体(RLO)可能是海湾扇贝在海上吊养的幼贝阶段发病死亡的致病因子.
- 海湾扇贝 /
- 流行病学调查 /
- 死亡率 /
- 类立克次体 /
- 类支原体
Abstract: A systemic epizo otiological study on mass mortalities of the cultured sea scallop Argopectenirra dians was carried out during the period of 2001~2002.The results show that the mortality rate of the juvenile population of A.irradians is about 60% from June to October,with the death peak occurring in June.The results on investig ation of environm ntal factors revealed that the lower water temperature might be the serious stress factor to the cultured juvenile populations of A.irr adians.Several suspicious pathogens infecting the scallop were found such as rickett sia-like or ganism(RLO),my coplasma-like organism(M L O).The results of histological observation showed that rickettsia-like org anism inclusions were common,histological widespread infective agent.The averaged prevalence was from 15%~80% among cultured juvenile populations with averaged sizes of 0.5~5 cm.The highest infection intensity of RLO was from averaged 11.5 to 16 inclusions persection during the death peak of juvenile populations.An inference is made from the above analysis that RLO infection is positively correlated with mor tality rates of juvenile populations and may be the main pathogen responsible for mass mortalit ies of the cultured juvenile populations of scallop,A.irradians on the basis of the obser vation of intensity of RLO infection and hostpathology.
- Argopecten irradians /
- epizootiology /
- rickettsia-like organism /
- mycoplasma-like organism /

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参考文献(21)

于瑞海,王如才,田传远,等.栉孔扇贝大面积死亡的原因分析及预防探讨[J],海洋湖沼通报,1998,(3):69-72.

张福绥,杨红生.山东沿岸夏季栉孔扇贝大规死亡原因分析[J],海洋科学,1999,(1):44-47.

过峰,孙耀,曲克明,等.日照石臼扇贝养殖水域夏季环境调查分析[J],海洋水产研究,1999,20(1):45-51.

WU,X Z,LIU,Y J,LI,D F,et al.Ultrastructure and histopathology of a prokaryotic organism causing systemic infection in the scallops Chlamys farreri and Argopecten irradians in China[J].Diseases of Aquatic Organisms,in press.2005

李登峰,孙敬锋,吴信忠.栉孔扇贝体内寄生的病毒的分离纯化及其形态学观察[J].2002,海洋学报,24(4):145-148.

张维翥,吴信忠.扇贝大规模死亡病原病因学研究进展及其防治策略[A].中国科学技术协会.病虫害防治绿皮书[M].北京:2003.166-170.

王秀华,王崇明,李筠,等.胶州湾栉孔扇贝大规模死亡的流行病学调查[J].水产学报,2002,26(2):149-155.

吴信忠,潘金培.热带海洋珍珠贝类立克次体病研究:Ⅳ.组织细胞病理学研究[J].海洋学报,1999,21(2):93-98.

吴信忠,潘金培.热带海洋珍珠贝立克次体病研究:Ⅴ.超微病理学及致病机理研究[J].海洋学报,21(3):113-118.

WU Xin-zhong,PAN J.An intracellular prokaryotic microorganism associated with lesions in the oyster Crassostrea ariakensis Gould[J].Journal of Fish Diseases,2000,23(6):409-414.

GULKA G,CHANG hang P W.Prokariotic infection associated with a mass mortality of the sea scallop,Placopectenmagellanicus[J].J Fish Diseases.1983,7(6):355-364.

GULKA G,PEI W C.Pathogenicity and infectivity of a Rickettsia-like organisms in the sea scallop,Placopecten magellanicus [J].J.Fish Diseases.1984,8(6):309-318.

LEIBOVITZ.Chlamidiosis:a newly reported serious disease of larval and postmetamorphic bay scallop Argopecten irridians Lamarck)[J].J Fish Diseases.1989,12(2):126-136.

王运涛,相建海.栉孔扇贝大规模死亡的原因探讨[J].海洋与湖沼,1999,30(6):770-774.

吴信忠,潘金培,江静波.热带海洋珍珠贝类立克次体(RLO)病研究:Ⅶ.大珠母贝育苗期幼虫群体RLO病流行病学研究[J].海洋学报,2002,24(6):67-75.

SUSAN E FORD,FRANCISCO J.Borrero,epizootiology and pathology of juvenile oyster disease in the eastern oyster,Crassostrea virginica[J].Journal of Invertebrate Pathology,2001,78(3):141-154.

AAEVEDO C,VILLALBA A.Extracellular giant rickettsiae associated with bacteria in the gill of Crassostrea gigas (Mollusca,Bivalvia)[J].Journal of Invertebrate Pathology,1991,58(1):75-81.

RENAULT T,COCHENNEC N.Rickettsia-like organisms in the cytoplasm of gill epithelial cells of the Pacific oyster Crassostrea gigas[J].Journal of Invertebrate Pathology,1994,64(2):160-162.

MEYERS T R.Endemic diseases of cultured shellfish of Long Island,New York:adult and juvenile American oysters (Crassostrea virginica) and hard clams (Mercenaria mercenaria) [J].Aquaculture,22 (4):350-330.

WU Xin-zhong,PAN J.Studies on rickettsia-like organism disease of the tropical marine pearl oyster.1.The fine structure and morphogenesis of Pinctada maxima pathogen rickettsia-like organism[J].Journal of Invertebrate Pathology,1999,73(2):162-167.

HINE P M,DIGGLES B K.Prokaryote infections in the New Zealand scallops Pecten novaezelandiae and Chlamys delicatula [J].Dis Aquat Org,2002,50(2):137-144.

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