海洋附着细菌对冠瘤海鞘幼体附着和变态的影响

张朝霞; 柯才焕; 冯丹青; 黄英; 周时强; 吕嘉扬

海洋附着细菌对冠瘤海鞘幼体附着和变态的影响

厦门大学, 海洋学系, 福建厦门 361005;亚热带海洋研究所, 福建厦门 361005;厦门大学, 海洋环境科学教育部重点实验室, 福建厦门 361005

基金项目: 国家自然科学基金资助项目(49976034).

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出版历程
- 收稿日期: 2004-08-20
- 修回日期: 2004-12-01

Influences of marine adhesive bacteria on settlement and metamorphosis of Styela conopus Savigny larvae

Department of Oceanography, Xiamen University, Xiamen 361005, China;Institute of Subtropic Oceanography, Xiamen 361005, China;Key Laboratory of Marine Environmental Science of the Ministry of Education, Xiamen University, Xiamen 361005, China

摘要

摘要: 从冠瘤海鞘、皱瘤海鞘及乳突皮海鞘的被囊表面及其附着基和附近的海水中分离到290株细菌,根据其形态和生理生化特征,将其鉴定至弧菌属、肠杆菌属和无色杆菌属等10个属.分析海鞘被囊表面及其附着基和海水中的细菌组成,并从各属中挑出的29株细菌中分离筛选出附着能力较强的9株.将这9株细菌分别制成不同的单种细菌黏膜及9种细菌混合黏膜,检测细菌黏膜对冠瘤海鞘幼体附着和变态的影响.结果表明不同细菌黏膜对冠瘤海鞘幼体的附着和变态起不同的作用.无色杆菌属H-13显著地促进海鞘的附着和变态;弧菌属的H-4对海鞘的附着和变态均起抑制作用;弧菌属H-9明显地促进附着却强烈抑制海鞘幼体变态;其余细菌仅仅对海鞘幼体附着或变态的一个过程起作用或是对两者都没有明显效果.
- 细菌黏膜 /
- 冠瘤海鞘幼体 /
- 附着 /
- 变态
Abstract: One hundred and eighty bacteria isolated from surface of tunics of Styelaconopus Savigny,Styela plicata Lesueur,Molgula manhattensis Delay and their adhesive substrates,as well as 110 ones from nearby water,were investigated.On the basis of their morphological,biochemical and physiological characteristics,these bacteria belong to 10 genera and over 60% bacteria are Gram-negative.In bacteria from surfaces of sea squirtps tunics and their adhesive substrates,dominant colonies are Pseudomonas,while Aeromonas are primary in bacteria from water.Nine strains were screened out from 180 bacteria for their greatability of attachment.Then these 9 bacteria were cultured to form different bacterial films respectively,to gether with a mixed artificial film made of all 9 strains so that influences of these ar tificial films on set tlement and metamo rphosis of Styel a conopus Savigny larvae were studied.It is revealed that different types of artificial films have dissimilar effects on set tlement and met amorphosis of Styela conopus Savigny larvae.On one hand,5 kinds of bact erial films,H6,H9,H13,H15 and the mixed film remarkably stimulate larva attachment,while H4 strongly inhibited and others are without evident influences.On the other hand,in 10 bact erial films,H4,H7,and H9 obviously restrain metam orphosis of lava of Styela conops Savigny,but more larva are metamor phic when they grew on H13 and the mixed bacterial film.It illustrated that set tlement and met amorphosis of Styel a conop us Savigny larva were two correspondingly independent processes during these two courses,different effects were shown when larva grew on the same bacterial film.
- bacterial film /
- ascidian /
- larvae /
- settlement

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参考文献(33)

SZEWZYK U,HOLMSTROM C,WRANGSTADH M,et al.Relevance of the exopolysaccharide of marine Pseudomonas sp.Strain S9 for the attachment of Ciona intestinalis larvae[J].Mar Ecol Prog Ser,1991,75(2-3):259-265.

WIECZOREK S K,TODD C D.Inhibition and facilitation of bryozoan and ascidian settlement by natural multi-species biofilms:effects of film age and the roles of active and passive larval attachment[J].Mar Biol,1997,128(3):463-473.

HOLMSTROM C,RITTSCHOF D,KJELLEBERG S.Inhibition of settlement by larvae of Balanus amphitrite and Ciona intestinalis by a surface-colonizing marine Bacterium[J].Appl Envirl Microbiol,1992,58(7):2 111-2 115.

KEOUGH M J,RAIMONDI P T.Responses of settling invertebrate larvae to bioorganic films:effects of different types of films[J].J Exp Mar Biol Ecol,1995,185(2):235-253.

中国科学院微生物研究所.一般细菌常用鉴定方法[M].北京:科学出版社,1978.111-126.

霍尔特J G.简明第八版伯杰细菌鉴定手册[M].山东:山东大学出版社,1988.78-224.

纪伟尚,许兵,徐怀恕,等.海洋细菌在生物表面和非生物表面附着的研究[J].青岛海洋大学学报,1991,21(2):60-68.

MAKI J S,RITTSCHOF D,SCHMIDT A R,et al.Factors controlling attachment of bryozoan Larvae:a comparison of bacterial films and unfilmed surfaces[J].Biol Bull,1989,177(2):295-302.

SVANE I,YOUNG C M.The Ecology and behaviour of ascidian larvae[J].Oceanogr.Mar Biol,1989,27(1):45-90.

DEGNAN B M,SOUTER D,DEGNAN S M,et al.Induction of metamorphosis with potassium ions requires development of competence and an anterior signaling centre in the ascidian Herdmania momus[J].Dev Genes Evol,1997,206(6):370-376.

徐怀恕,许兵,纪伟尚.扇贝幼虫附着基的细菌组成及其作用[J].水产学报,1991,15(2):117-123.

林燕顺,叶德赞,姚瑞梅,等.海洋微型附着生物的研究——厦门港微型附着生物群落的形成和演替(冬季)[J].海洋通报,1983,2(3):45-54.

LAU S C K,THIYAGARAJAN V,OIAN P Y.The bioactivity of bacterial isolates in Hong Kong waters for the inhibition of barnacle (Balanusamphitrite Darwin) settlement[J].J Exp Mar Biol Ecol,2003,282(1-2):43-60.

WOOLLACOTT R M,HADFIELD M G.Induction of metamorphosis in larvae of a sponge[J].InvertBiol,1996,115(4):257-262.

MULLER WA,WIECKER F,EIBEN R.Larval adhesion,releasing stimuli and metamorphosis.MACKIE.Coelenterate Ecology and Behavior[M].New York:Plenum Press,1976.339-346.

HOFMANN D K,NEUMANN R,HENNE K.Strobilation,budding and initiation of scyphistoma morphogenesis in the rhizostome Cassiopeaandromeda (Cnidaria:Scyphozoa)[J].Mar Biol,1978,47(2):161-176.

NEUMANN R.Bacterial induction of settlement and metamorphosis in the planula larvae of Cassiopea andromeda (Cnidaria:Scyphozoa,Rhizostomeae)[J].Mar Ecol Prog Ser,1979,(1):21-28.

SCHELTEMA R S.Metamorphosis of the veliger larvae of Nassarius obsoletus (Gastropoda) in response to bottom sediment[J].Biol Bull,1961,120(1):92-109.

CAMERON R A,HINEGARDNER R T.Initiation of metamorphosis in laboratory cultured sea urchins[J].Biol Bull,1974,146(3):335-342.

JOHNSON C R,SUTTON D C,OLSON R R,et al.Settlement of crown-of-thorns starfish:role of bacteria on surfaces of coralline algae and a hypothesis for deepwater recruitment[J].Mar Ecol Prog Ser,1991,71(2):143-162.

HERRMANN K.Influence of bacteria in inducing metamorphosis and the course of metamorphosis in Actinotrocha branchiata [J].Verh Zool Ges Wilhelmshaven,1975,1974:112-115.

MIHM J W,BANTA W C,Loeb G I.Effects of adsorbed organic and primary fouling films on bryozoan settlement[J].J Exp Mar Biol Ecol,1981,54(2):167-179.

BRANCATO M S,WOOLLACOTT R M.Effect of microbial films on settlement of bryozoan larvae(Bugula simplex,B stolonifera and B.turrita)[J].MarBiol,1982,71(1):51-56.

KIRCHMAN D,GRAHAM S,REISH D,et al.Bacteria induce settlement and metamorphosis of Janua (Dexiospira) brasiliensis Grube(Polychaeta:Spirorbidae)[J].J Exp Mar Biol Ecol,1982,56(2):153-163.

HADFIELD M G,UNABIA C C,SMITH C C,et al.Settlement preferences of the ubiquitous fouler Hydroides elegans.THOMPSON M F,NAGABUSHANAM,SAROJINI R,et al.Recent developments in Biofouling Control[M].New Delhi:Oxford and IBH,1994.65-74.

UNABIA C R C,HADFIELD M G.Role of bacteria in larval settlement and metamorphosis of the polychaete Hydroideselegans[J].Mar Biol,1999,133(1):55-64.

O'CONNOR N J,RICHARDSON D L.Attachment of barnacle (Balanusamphitrite Darwin) larvae:responses to bacterial films and extracellular materials[J].J Exp Mar Biol Ecol,1998,226(1):115-129.

MAKI J S,RITTSCHOF D,SAMUELSSON M O,et al.Effect of marine bacteria and their exopolymers on the attachment of barnacle cypris larvae[J].Bull Mar Sci,1990,46(2):499-511.

WEINER R M,SEGALL A M,COLWELL R R.Characterization of a marine bacterium associated with Crassostrea virginica (the Eastern oyster) [J].Appl envirl Microbiol,1985,49(1):83-90.

DOUILLET P,LANGDON C J.Effects of marine bacteria on the culture of axenic oyster Crassostrea gigas (Thunberg) Larvae[J].Biol Bull,1993,184(1):36-51.

生菊,许兵,纪伟尚,等.海洋细菌对海湾扇贝幼虫附着的影响[J].青岛海洋大学学报,1994,24(增刊):149-156.

MAKI J S,RITTSCHOF D,COSTLOW J D,et al.Inhibition of attachment of larval barnacles,Balanus amphitrite,by bacterial surface films[J].Mar Biol,1988,97(2):199-206.

ZHAO Bin,QIAN Pei-yuan.Larval settlement and metamorphosis in the slipper limpet Crepidula onyx (Sowerby) in response to conspecific cues and the cues from biofilm[J].J Exp Mar Biol Ecol,2002,269(1):39-51.

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